壳聚糖在细菌纤维素基支架皮肤组织工程中的抗菌效果

Proceedings of the 1st International Conference on Chemical Science and Technology Innovation Pub Date : 2019-01-01 DOI:10.5220/0008864701780181

Khatarina Meldawati Pasaribu, S. Gea, S. Ilyas, Tamrin

{"title":"壳聚糖在细菌纤维素基支架皮肤组织工程中的抗菌效果","authors":"Khatarina Meldawati Pasaribu, S. Gea, S. Ilyas, Tamrin","doi":"10.5220/0008864701780181","DOIUrl":null,"url":null,"abstract":"It is recognized that bacterial cellulose (BC) is used as a scaffold for tissue engineering. However, pristine BC is not ideal enough to be applied as a scaffold because bacterial cellulose does not have antimicrobial activity. The aim of this study was to evaluate the antmicrobial activity of bacterial cellulose and their composites. BC gel, produced by Acetobacter xylinum with HS medium as a carbohydrate resources, was immersed into chitosan (Ch) and collagen (Co) by ex-situ approach to produce BC/Ch/Col. The same procedures were repeated for BC/Ch, BC/Col, and BC/Col/Ch. The effectiveness of antimicrobial activity was carried out using disk paper to inhibit the growth of pathogen bacteria such as Escherichia coli and Staphylococcus aureus. The results showed that BC/Ch has the highest antimicrobial activity against E. coli and S. aureus with the inhibition zone of 10.15 mm and 7.9 mm, respectively.","PeriodicalId":20533,"journal":{"name":"Proceedings of the 1st International Conference on Chemical Science and Technology Innovation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"The Effectiveness of Chitosan as an Antimicrobial on Bacterial Cellulose-based Scaffold Skin Tissue Engineering\",\"authors\":\"Khatarina Meldawati Pasaribu, S. Gea, S. Ilyas, Tamrin\",\"doi\":\"10.5220/0008864701780181\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"It is recognized that bacterial cellulose (BC) is used as a scaffold for tissue engineering. However, pristine BC is not ideal enough to be applied as a scaffold because bacterial cellulose does not have antimicrobial activity. The aim of this study was to evaluate the antmicrobial activity of bacterial cellulose and their composites. BC gel, produced by Acetobacter xylinum with HS medium as a carbohydrate resources, was immersed into chitosan (Ch) and collagen (Co) by ex-situ approach to produce BC/Ch/Col. The same procedures were repeated for BC/Ch, BC/Col, and BC/Col/Ch. The effectiveness of antimicrobial activity was carried out using disk paper to inhibit the growth of pathogen bacteria such as Escherichia coli and Staphylococcus aureus. The results showed that BC/Ch has the highest antimicrobial activity against E. coli and S. aureus with the inhibition zone of 10.15 mm and 7.9 mm, respectively.\",\"PeriodicalId\":20533,\"journal\":{\"name\":\"Proceedings of the 1st International Conference on Chemical Science and Technology Innovation\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2019-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Proceedings of the 1st International Conference on Chemical Science and Technology Innovation\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.5220/0008864701780181\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Proceedings of the 1st International Conference on Chemical Science and Technology Innovation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.5220/0008864701780181","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

摘要

细菌纤维素(BC)是组织工程中常用的支架材料。然而，由于细菌纤维素不具有抗菌活性，原始BC不足以作为支架应用。研究了细菌纤维素及其复合材料的抑菌活性。以HS培养基为碳水化合物资源，以木醋杆菌为原料制备BC凝胶，采用离地法制备壳聚糖(Ch)和胶原蛋白(Co)，得到BC/Ch/Col。对BC/Ch、BC/Col和BC/Col/Ch重复相同的程序。考察了纸对大肠杆菌、金黄色葡萄球菌等病原菌的抑菌效果。结果表明，BC/Ch对大肠杆菌和金黄色葡萄球菌的抑菌活性最高，抑菌带分别为10.15 mm和7.9 mm。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

The Effectiveness of Chitosan as an Antimicrobial on Bacterial Cellulose-based Scaffold Skin Tissue Engineering

It is recognized that bacterial cellulose (BC) is used as a scaffold for tissue engineering. However, pristine BC is not ideal enough to be applied as a scaffold because bacterial cellulose does not have antimicrobial activity. The aim of this study was to evaluate the antmicrobial activity of bacterial cellulose and their composites. BC gel, produced by Acetobacter xylinum with HS medium as a carbohydrate resources, was immersed into chitosan (Ch) and collagen (Co) by ex-situ approach to produce BC/Ch/Col. The same procedures were repeated for BC/Ch, BC/Col, and BC/Col/Ch. The effectiveness of antimicrobial activity was carried out using disk paper to inhibit the growth of pathogen bacteria such as Escherichia coli and Staphylococcus aureus. The results showed that BC/Ch has the highest antimicrobial activity against E. coli and S. aureus with the inhibition zone of 10.15 mm and 7.9 mm, respectively.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Proceedings of the 1st International Conference on Chemical Science and Technology Innovation

自引率

0.00%

发文量