放线菌色素辅助纳米粒子合成及其生物活性研究

Naresh Kumar Singh, B. Naik, Vijay Kumar, Vivek Kumar, Sanjay Gupta
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引用次数: 4

摘要

近年来,纳米颗粒的绿色合成因其具有成本效益、简单、生态友好、生物相容性等优点,以及相对于传统的化学和物理技术具有广泛的应用前景,受到了广泛的关注。从野生胡萝卜根际土壤中分离到20株放线菌,并对其产色素能力进行了筛选。这些分离物属于链霉菌属(58%),其次是链孢菌属(19%)、诺卡菌属(11%)、放线菌属(8%)和小单孢菌属(4%)。最有前途的分离物(NS-05)产生的粉红色色素已被用于合成纳米银。根据培养特性和16S rDNA序列分析,鉴定菌株NS-05为Streptomyces sp.。与型菌株富vissimus DSM 40593T、S. microflavus NBRC13062T、S. setonii NRRL ISP-5322T、S. anulatus RRL B-2000T亲缘关系最近,序列相似性为95.6%,提示其可能属于链霉菌新种。利用紫外可见光谱(UV-Vis)、红外光谱(FTIR)和扫描电镜对生物色素辅助合成的纳米颗粒进行了表征。合成的银纳米粒子的平均尺寸为42.5nm, λ max为433 nm。合成的纳米颗粒对金黄色葡萄球菌MTCC 2940、枯草芽孢杆菌MTCC 441、伤寒沙门氏菌、普通变形杆菌MTCC 6380、大肠杆菌MTCC 739等主要病原菌具有良好的抗氧化活性。目前的研究结果是有希望的,这种色素也可以用于其他纳米颗粒的绿色合成。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ACTINOBACTERIAL PIGMENT ASSISTED SYNTHESIS OF NANOPARTICLES AND ITS BIOLOGICAL ACTIVITY
Recently, the green synthesis of nanoparticles has gained considerable attention due to its benefits such as cost efficiency, simplicity, eco-friendly nature, biocompatibility and broad applications over conventional chemical and physical techniques. In this context twenty actinobacteria were isolated from the rhizospheric soil of wild carrot and screened for their pigment producing ability. These isolates belong to the genus Streptomyces (58%), followed by Streptosporangium sp.  (19%), Nocardia sp. (11%), Actinomadura sp. (8%), and Micromonospora sp. (4%). The most promising isolate (NS-05) producing the pink pigment has been taken for the synthesis of silver nanoparticles. The isolate NS-05 was identified as Streptomyces sp. based on cultural characteristics and 16S rDNA sequence analysis. It was most closely related with type strain Streptomyces fulvissimus DSM 40593T, S. microflavus NBRC13062T, S. setonii NRRL ISP-5322T, S. anulatus RRL B-2000T with a sequence similarity of 95.6% which shows that it may belong to novel species of Streptomyces.  The bio-pigment assisted synthesized nanoparticles were characterized using UV-Vis, FTIR and Scanning electron microscopy studies. The average size of synthesized silver nanoparticles was 42.5nm and has λ max at 433 nm.  The synthesized nanoparticles showed promising activity against major pathogens like Staphylococcus aureus MTCC 2940, Bacillus subtilis MTCC 441 Salmonella typhi, Proteus vulgaris MTCC 6380, Escherichia coli MTCC 739.  The findings of present research are promising, and this pigment can also be used for the green synthesis of other nanoparticles.
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