四种不同炮制法人参皂苷在大鼠体内含量及药动学的相关性研究

IF 0.4 Q4 SPECTROSCOPY
Ji-Hyeon Jeon, Jaehyeok Lee, C. Lee, Min‐Koo Choi, I. Song
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引用次数: 2

摘要

以1 g/kg剂量口服四种人参制品,比较其在大鼠体内人参皂苷含量及药代动力学的变化。四种人参产品分别为新鲜人参提取物、红参提取物、白参提取物和从人参根中提取的富含皂苷的白参提取物。采用液相色谱-串联质谱法(LC-MS/MS)测定人参制品和大鼠血浆样品中人参皂苷的含量。15种人参皂苷中检出8、9种人参皂苷;但人参总皂苷的含量因制备方法的不同而不同。此外,三糖基化人参皂苷的含量高于二糖基化人参皂苷,并且在任何制剂中均不存在去糖基化人参皂苷。大鼠单次口服四种人参产品后,15种人参皂苷中仅检测到20(S)种人参皂苷Rb1 (GRb1)、GRb2、GRc和GRd等4种人参皂苷。GRb1、GRb2、GRc和GRd的血浆浓度因制备方法的不同而不同,但四种人参制品的药动学特征相似。综上所述,人参制品中GRb1、GRb2和GRc的含量与浓度曲线下面积呈良好的相关性,而与GRd无关,这可能与人参制品中GRb1、GRb2和GRc含量较高及肠道生物转化有关。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Correlation between the Content and Pharmacokinetics of Ginsenosides from Four Different Preparation of Panax Ginseng C.A. Meyer in Rats
We aimed to compare the content of ginsenosides and the pharmacokinetics after the oral administration of four different ginseng products at a dose of 1 g/kg in rats. The four different ginseng products were fresh ginseng extract, red ginseng extract, white ginseng extract, and saponin enriched white ginseng extract prepared from the radix of Panax ginseng C.A. Meyer. The ginsenoside concentrations in the ginseng product and the rat plasma samples were determined using a liquid chromatography-tandem mass spectrometry (LC-MS/MS). Eight or nine ginsenosides of the 15 tested ginsenosides were detected; however, the content and total ginsenosides varied depending on the preparation method. Moreover, the content of triglycosylated ginsenosides was higher than that of diglycosylated ginsenosides, and deglycosylated ginsenosides were not present in any preparation. After the single oral administrations of four different ginseng products in rats, only four ginsenosides, such as 20(S)ginsenosides Rb1 (GRb1), GRb2, GRc, and GRd, were detected in the rat plasma samples among the 15 ginsenosides tested. The plasma concentrations of GRb1, GRb2, GRc, and GRd were different depends on the preparation method but pharmacokinetic features of the four ginseng products were similar. In conclusion, a good correlation between the area under the concentration curve and the content of GRb1, GRb2, and GRc, but not GRd, in the ginseng products was identified and it might be the result of their higher content and intestinal biotransformation of the ginseng product.
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来源期刊
CiteScore
0.90
自引率
20.00%
发文量
0
审稿时长
6 weeks
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