用于双色双光子激光扫描显微镜的新型荧光蛋白对：用于双色双光子激光扫描显微镜的新型荧光蛋白对

Pub Date : 2012-11-23 DOI:10.3724/SP.J.1206.2012.00027

Song Yang, Yan Teng, P. Xu

{"title":"用于双色双光子激光扫描显微镜的新型荧光蛋白对*：用于双色双光子激光扫描显微镜*的新型荧光蛋白对","authors":"Song Yang, Yan Teng, P. Xu","doi":"10.3724/SP.J.1206.2012.00027","DOIUrl":null,"url":null,"abstract":"Dual-color two-photon laser scanning microscopy is a useful method for simultaneously studying the expression, localization and trafficking of two different proteins in tissues. Because most two-photon microscopes only use a single wavelength excitation laser, simultaneously exciting multiple fluorescent proteins remains a challenge. Here, we present mAmetrine and mKate2, which can be used as a novel fluorescent protein pair in dual-color two-photon imaging by taking advantage of the large Stokes shift of mAmetrine and high brightness of mKate2. Both proteins have high two-photon absorption efficiencies and can be simultaneously excited at an optical wavelength of 765 nm. Dual-color two-photon imaging using this protein pair is highly effective in living cells.","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-11-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A Novel Fluorescent Protein Pair for Dual-color Two-photon Laser Scanning Microscopy*: A Novel Fluorescent Protein Pair for Dual-color Two-photon Laser Scanning Microscopy*\",\"authors\":\"Song Yang, Yan Teng, P. Xu\",\"doi\":\"10.3724/SP.J.1206.2012.00027\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Dual-color two-photon laser scanning microscopy is a useful method for simultaneously studying the expression, localization and trafficking of two different proteins in tissues. Because most two-photon microscopes only use a single wavelength excitation laser, simultaneously exciting multiple fluorescent proteins remains a challenge. Here, we present mAmetrine and mKate2, which can be used as a novel fluorescent protein pair in dual-color two-photon imaging by taking advantage of the large Stokes shift of mAmetrine and high brightness of mKate2. Both proteins have high two-photon absorption efficiencies and can be simultaneously excited at an optical wavelength of 765 nm. Dual-color two-photon imaging using this protein pair is highly effective in living cells.\",\"PeriodicalId\":0,\"journal\":{\"name\":\"\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0,\"publicationDate\":\"2012-11-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.3724/SP.J.1206.2012.00027\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.3724/SP.J.1206.2012.00027","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 0

摘要

双色双光子激光扫描显微镜是同时研究两种不同蛋白在组织中的表达、定位和转运的有效方法。由于大多数双光子显微镜只使用单一波长的激发激光，因此同时激发多个荧光蛋白仍然是一个挑战。本文提出了mAmetrine和mKate2，利用mAmetrine的Stokes位移大和mKate2的高亮度，可以作为一种新的荧光蛋白对用于双色双光子成像。这两种蛋白质都具有较高的双光子吸收效率，可以在765 nm的光波长下同时被激发。双色双光子成像使用这种蛋白质对是非常有效的活细胞。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文

A Novel Fluorescent Protein Pair for Dual-color Two-photon Laser Scanning Microscopy*: A Novel Fluorescent Protein Pair for Dual-color Two-photon Laser Scanning Microscopy*

Dual-color two-photon laser scanning microscopy is a useful method for simultaneously studying the expression, localization and trafficking of two different proteins in tissues. Because most two-photon microscopes only use a single wavelength excitation laser, simultaneously exciting multiple fluorescent proteins remains a challenge. Here, we present mAmetrine and mKate2, which can be used as a novel fluorescent protein pair in dual-color two-photon imaging by taking advantage of the large Stokes shift of mAmetrine and high brightness of mKate2. Both proteins have high two-photon absorption efficiencies and can be simultaneously excited at an optical wavelength of 765 nm. Dual-color two-photon imaging using this protein pair is highly effective in living cells.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助