一种新型直接饲养肉牛微生物对产气荚膜梭菌具有体外和体内支持作用

Q4 Veterinary
O. Guimaraes, B. Cappellozza, Lena C. Capern, J. Schutz, C. Cull, O. Queiroz, G. Copani
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引用次数: 1

摘要

研究了一种含有动物乳杆菌LA-51、弗氏丙酸杆菌PF-24、地衣芽孢杆菌CH-200和枯草芽孢杆菌King (BOVAMINE DEFEND®Plus)的新型直饲微生物(DFM)对产气荚膜梭菌病原菌的体外和体内作用。在实验1(体外)中,采用琼脂扩散法定性评价了DFM对A型和c型产气荚膜荚膜菌的体外抑制效果。在96孔板中,DFM产生了大于3个环直径的抑制区。试验2(体内)选取20头1日龄肉牛,分为对照组(n = 10)和DFM组(n = 10),为期21 d。每头犊牛口服产气荚膜荚膜球菌A型S-107菌落形成单位1.0 × 108个。在产气荚膜梭菌感染后进行一般健康评分、体重和粪便样本收集等程序。日饲DFM显著降低了腹泻发生率,改善了犊牛的总体印象和外观评分。总之,这些结果突出了在不同的实验环境下,含有L. animal alis LA-51、P. freudenreichii PF-24、B. licheniformis CH-200和B. subtilis (BOVAMINE DEFEND®Plus)的DFM对A型和C型产气荚膜荚膜杆菌的抑制能力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A Novel Direct-Fed Microbial for Beef Cattle Has a Supportive Effect against Clostridium perfringens In Vitro and In Vivo
Two experiments were conducted to evaluate the in vitro and in vivo effects of a novel direct-fed microbial (DFM) containing Lactobacillus animalis LA-51, Propionibacterium freudenreichii PF-24, Bacillus licheniformis CH-200, and Bacillus subtilis King (BOVAMINE DEFEND® Plus) against Clostridium perfringens pathogenic strains. In Experiment 1 (in vitro), an agar diffusion assay was performed to qualitatively evaluate the in vitro inhibitory effects of the DFM against C. perfringens types A and C. Including the DFM in the tested yielded inhibition zones with greater than three ring diameters in a 96-well plate. In Experiment 2 (in vivo), twenty 1-day-old beef calves were allocated to control (n = 10) or DFM (n = 10) for 21 days. All calves were orally challenged with 1.0 × 108 colony forming units of C. perfringens type A strain S-107 per head. The procedures such as general health scores, body weight, and fecal sample collections were performed following the C. perfringens challenge. Daily feeding of DFM significantly reduced the incidence of diarrhea while improving general impression and appearance scores of calves. Overall, these results highlight the ability of the DFM containing L. animalis LA-51, P. freudenreichii PF-24, B. licheniformis CH-200, and B. subtilis (BOVAMINE DEFEND® Plus) to inhibit C. perfringens types A and C under different experimental settings.
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来源期刊
Indian Journal of Small Ruminants
Indian Journal of Small Ruminants Veterinary-Small Animals
CiteScore
0.40
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0.00%
发文量
81
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