天然转基因生物中章鱼碱/鱼碱合成酶基因的同源物

Q3 Agricultural and Biological Sciences
Anton D. Shaposhnikov, T. Matveeva
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引用次数: 0

摘要

水平基因转移的过程导致了自然转基因生物的出现。目前,已知超过7%的双子叶植物物种是天然转基因的,即nGMO bb0。这些植物含有农杆菌的基因,这些基因在感染过程中被整合到核基因组中。在一些自然转基因植物物种中,农杆菌基因已经进化了数百万年。在这些基因中,编码章鱼碱/鱼碱合成酶(ocs/vis)的基因可以被区分出来。研究天然转基因植物中章鱼素/vitopin合成酶基因的同源物及其结构和多样性,以及编码酶的产物,将有助于我们确定同源物在nGMO中的功能和进化作用。目前,生物信息学和基因工程方法被用来解决这些问题。在7种中发现了ocs\vis-like:合欢和Durazz;,锥体柱头(图1)加格农·g·p·刘易斯《泡桐》Hemsl。;窄翅翼龙;、地黄。在17个nGMO物种中,共发现了21个ocs/vis序列。20个序列是完整的。这可能表明这些基因在nGMO中的功能意义。对目前已知的农杆菌、根瘤菌和天然转基因生物的ocs/vis样基因的系统发育分析表明,所研究基因的多样性比基于农杆菌序列估计的要广泛。在邻域连接法构建的系统发育树上,可以区分出6个ocs/vis类。其中3个簇含有nGMOs和农杆菌,表明nGMOs的T-DNA序列与目前已知的农杆菌/根瘤菌菌株的T-DNA序列存在相关性。三个集群只包含ngmo。其中一个由属于大麻科的物种组成。其他集群是异构的。所研究的物种之间没有明显的生态相似性。所得结果可用于研究古代和现代农杆菌菌株的多样性、寄主特异性及其基因在植物进化中的可能作用。这项工作得到了RSF,拨款21-14-00050和圣彼得堡国立大学分子和细胞技术研究资源中心的支持。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Homologues of octopine/vitopine synthase genes in natural GMOs
The process of horizontal gene transfer causes the appearance of natural genetically modified organisms. At the moment, it is known that over 7% of dicotyledonous plant species are naturally transgenic, i.e. nGMO [1]. These plants contain the genes of agrobacteria, which are integrated in the nuclear genome during infection. In some species of naturally transgenic plants, agrobacterial genes have been preserved for millions of years of evolution. Among these genes, genes encoding octopine/vitopine synthase (ocs/vis) can be distinguished [2]. The study of homologues of octopin/vitopin synthase genes in naturally transgenic plants: their structures and diversity, products of encoded enzymes will allow us to establish the functions and evolutionary role of homologues in nGMO. Currently, bioinformatic and genetic engineering methods are used to solve these problems. ocs\vis-like were found in 7 species: Albizia julibrissin Durazz., Cenostigma pyramidale (Tul.) Gagnon G.P.Lewis, Paulownia fortunei (Seem.) Hemsl., Pterocarya stenoptera C.DC., Rehmannia glutinosa Steud., Santalum album L., Viscum album L. In total twenty one ocs/vis sequences are known in 17 nGMO species. Twenty sequences are intact. This may indicate the functional significance of these genes for nGMO. Phylogenetic analysis of currently known ocs/vis-like genes of Agrobacterium, Rhizobium and natural GMOs suggests that diversity of studied genes is wider, than it was estimated based on agrobacterial sequences. On the phylogenetic tree constructed by the neighbor-joining method, 6 clusters for ocs/vis can be distinguished. Three clusters contain nGMOs and agrobacteria, showing the relationship of the T-DNA sequences of nGMO with those of currently known strains of of Agrobacterium/Rhizobium. Three clusters contain only nGMOs. One of them consists of species that belong to the Cannabaceae family. Other clusters are heterogeneous. No significant ecological similarities were found among the studied species. The obtained results can be used to study the diversity of ancient and modern strains of agrobacteria, their host specificity and the possible role of their genes in plant evolution. The work was supported by the RSF, grant 21-14-00050 and Research Resource Center for molecular and cellular technologies of Saint Petersburg State University.
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来源期刊
Ecological genetics
Ecological genetics Environmental Science-Ecology
CiteScore
0.90
自引率
0.00%
发文量
22
期刊介绍: The journal Ecological genetics is an international journal which accepts for consideration original manuscripts that reflect the results of field and experimental studies, and fundamental research of broad conceptual and/or comparative context corresponding to the profile of the Journal. Once a year, the editorial Board reviews and, if necessary, corrects the rules for authors and the journal rubrics.
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