Magnetic tweezers manipulate DNA slowdown based on nanopores

As a nano-sensor, solid-state nanopores have demonstrated their high sensitivity in the field of rapid detection of single molecules, but they still face many challenges in distinguishing the internal bases of DNA. Driven by voltage, the speed of DNA passing through the nanopore is very fast. When the voltage is 200mv, the via speed of DNA reaches 30base/us. At such a speed, the current change caused by a single base is difficult to be used by existing instruments. catch. Effectively reducing the speed of DNA through nanopores has become a major challenge. In this paper, the magnetic tweezers system platform was designed and constructed; the biobeads-streptavidin was connected between the magnetic beads and DNA, and the influence of the bias voltage on the structure of the magnetic beads-DNA was studied; the magnetic beads were manipulated using the magnetic tweezers system , Actively control DNA to reverse through nanopores, significantly reducing the speed of DNA passing through solid nanopores, and achieving active control of the speed of DNA passing through solid nanopores; solving key technical problems in detecting single bases in solid nanopores Provides ideas.