Studies on the Metabonomics of Premenstrual Syndrome Liver-Qi Invasion Patient Interfered by Jingqianping Granules

This work deals with the variation of urinary metabolite of patients suffering from premenstrual syndrome (PMS) and the function of Jingqianping granules (JG) in intervening liver-Qi invasion by metabonomic. The metabonomic characteristics and the micromolecular marking compound, which are closely related to the pathogenesis of PMS liver-Qi invasion cases, are also investigated. PMS liver-Qi invasion cases are calibrated according to the diagnostic criteria, including criteria, and excluding criteria. The urine samples from PMS liver-Qi invasion patients are analyzed at different times using UPLC-Q-TOF in combination with PCA pattern analysis, followed by discrimination of the metabolic outline and searching for the possible biological tags and metabolic pathways. The metabolic outline of premenstrual urine samples has significant difference among the control group, the PMS liver-Qi invasion group, and the PMS liver-Qi invasion treatment group. The metabolic outline of premenstrual urine sample of the PMS liver-Qi invasion group diverges from the premenstrual and postmenstrual urine samples of the control group and the postmenstrual urine sample of the PMS liver-Qi invasion group. Compared with the control group, N-acetyl-2-glutamate-5-semialdehyde has decreased greatly in the N-acetyl-2-glutamate-5-semialdehyde liver-Qi invasion group, and histidine and 3-methoxy-4-hydroxymandelaldehyde have increased significantly. This research, for the first time, deals with the metabolic pattern differences between the control group and the administration group. It shows that JG may repair metabolic disturbance of endogenous micromolecules in PMS liver-Qi invasion cases in terms of metabonomics. It also demonstrates that symptoms present before menstruation in PMS patients and the characteristic of PMS is that the symptoms appear before menstruation and disappear after it among PMS liver-Qi invasion cases.