葡聚糖-聚丙烯酰胺聚合物对肝脏和脾脏形态的影响及其作为银和金纳米粒子载体的作用

IF 0.2 Q4 MEDICINE, GENERAL & INTERNAL
О.М. Kaleinikova, Valentina Kurovska, І.V. Byelinska, N. Kutsevol, Т.V. Blashkiv
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引用次数: 0

摘要

聚合物纳米复合材料的应用前景正在深入研究,目的是将其应用于医学,特别是肿瘤学领域。在实验阶段,重要的是确定这些化合物对人体的影响机制及其本身可能产生的不良影响。目的研究最大剂量葡聚糖-聚丙烯酰胺聚合物的治疗效果及其作为银和金纳米颗粒载体对脾脏和肝脏的影响。用苏木精-伊红染色标准法对脾脏和肝脏微制剂进行组织学检查。由于用非离子(D-g-PAA)和阴离子(D-g-PAA (PE))聚合物基质处理,脾脏发生的变化表明所有血细胞的产生都增加了。当在基体中加入银和金纳米粒子时,这些现象就不存在了。在肝脏中,用D-g-PAA和D-g-PAA (PE)治疗可引起肝循环紊乱、炎症细胞局灶性浸润和肝细胞坏死死亡。纳米颗粒的加入引发了其他机制的改变,表现为糖原的过度积累,肝细胞的脂肪浸润和细胞死亡,主要是通过细胞凋亡。然而,与此同时,肝脏不完全再生反应的迹象被揭示出来。用最大剂量的试验物质处理所引起的形态学变化表明它们的毒性作用,特别是对肝脏的毒性作用。需要进一步的研究来确定最佳剂量和给药频率,这些剂量和频率可用于治疗目的,包括所研究的聚合物与血细胞的相互作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Morphological changes of liver and spleen under the impact of dextran-polyacrylamide polymers and their effects as carriers of silver and gold nanoparticles
The possibility of usage of polymer nanocomposites is being intensively studied today with a purpose of their application in medicine, espessialy in oncology. At the experimental stage it is important to determine the mechanisms of the influence of such compounds on the body and their own possible undesirable effects. Aim – to study the effect of the treatment with maximal doses of the dextran-polyacrylamide polymers and their effect as carriers of silver and gold nanoparticles on the spleen and liver. Histological examination of micropreparations of the spleen and liver by the standard method with hematoxylin-eosin staining was made. As a result of the treatment with nonionic (D-g-PAA) and anionic (D-g-PAA (PE)) polymer matrices, changes which occurred in the spleen indicate an increase in the production of all blood cells. These phenomena were absent when silver and gold nanoparticles were included in the matrix. In the liver, treatment with D-g-PAA and D-g-PAA (PE) caused a disorder of hepatic circulation, focal infiltration by inflammatory cells and death of hepatocytes by necrosis. The addition of nanoparticles triggered other mechanisms of alteration, which manifested themselves in excessive accumulation of glycogen, fatty infiltration of hepatocytes, and cell death, mainly through apoptosis. However, along with this, signs of an incomplete regenerative response of the liver were revealed. Morphological changes caused by the treatment with maximal doses of the tested substances indicate their toxic effect, especially on the liver. Further researches are needed to establish the optimal doses and the frequency of their administration, which can be used for therapeutic purposes, including the interaction of studied polymers with blood cells.
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来源期刊
Medical Perspectives-Medicni Perspektivi
Medical Perspectives-Medicni Perspektivi MEDICINE, GENERAL & INTERNAL-
CiteScore
0.40
自引率
0.00%
发文量
85
审稿时长
9 weeks
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