芽孢杆菌属内生细菌的特性及其对玉米(zea mays)生长的影响

Nikola Lipková, R. Cinkocki, J. Maková, Juraj Medo, S. Javoreková
{"title":"芽孢杆菌属内生细菌的特性及其对玉米(zea mays)生长的影响","authors":"Nikola Lipková, R. Cinkocki, J. Maková, Juraj Medo, S. Javoreková","doi":"10.15414/JMBFS.3602","DOIUrl":null,"url":null,"abstract":"Bacteria with positive properties on plant vitality are also called PGPB (Plant Growth Promoting Bacteria). Their presence can be observed not only in the root area but also in the above-ground parts of plants like endophytic bacteria. The aim of our study was to characterize promoting features of bacteria from Bacillus genus and compare them with Pseudomonas simiae WCS417 (plant growth promoting strain). The work was carried out in locality Koliňany near Nitra (40°26´46´´N, 79°58´56´´W) and root samples were taken from 6 randomly selected plants of maize (Zea mays L.) in vegetative plant growth stage BBCH 14-15. Bacteria isolated from plant roots were identified and tested to biochemical parameters. From the biochemical features, we observed the detection of siderophores, determination of indole-3-acetic acid (IAA), monitoring the ability to dissolve phosphates and antifungal activity. Bacterial suspensions were applied to maize seeds and tested in vivo controlled conditions. Tested isolates were identified as Bacillus flexus, Bacillus megaterium and Bacillus subtilis. All 3 strains achieved the middle – class of phosphate solubilization index (2.00 ≤ SI ˂ 4.00), produced phytohormone IAA and showed positive production of siderophores and inhibited growth of Fusarium culmorum to more than 50%. All differences between tested strains and control strain P. simiae WCS417 were also statistically confirmed. All strains showed positive results in monitoring plant growth promoting properties. The effect of three Bacillus strains on maize seeds in vivo conditions showed significant differences in root length (P<0.0001) and weight of the young plant (P<0.001) compared to control.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":"24 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2021-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"5","resultStr":"{\"title\":\"CHARACTERIZATION OF ENDOPHYTIC BACTERIA OF THE GENUS BACILLUS AND THEIR INFLUENCE ON THE GROWTH OF MAIZE (ZEA MAYS) IN VIVO\",\"authors\":\"Nikola Lipková, R. Cinkocki, J. Maková, Juraj Medo, S. Javoreková\",\"doi\":\"10.15414/JMBFS.3602\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Bacteria with positive properties on plant vitality are also called PGPB (Plant Growth Promoting Bacteria). Their presence can be observed not only in the root area but also in the above-ground parts of plants like endophytic bacteria. The aim of our study was to characterize promoting features of bacteria from Bacillus genus and compare them with Pseudomonas simiae WCS417 (plant growth promoting strain). The work was carried out in locality Koliňany near Nitra (40°26´46´´N, 79°58´56´´W) and root samples were taken from 6 randomly selected plants of maize (Zea mays L.) in vegetative plant growth stage BBCH 14-15. Bacteria isolated from plant roots were identified and tested to biochemical parameters. From the biochemical features, we observed the detection of siderophores, determination of indole-3-acetic acid (IAA), monitoring the ability to dissolve phosphates and antifungal activity. Bacterial suspensions were applied to maize seeds and tested in vivo controlled conditions. Tested isolates were identified as Bacillus flexus, Bacillus megaterium and Bacillus subtilis. All 3 strains achieved the middle – class of phosphate solubilization index (2.00 ≤ SI ˂ 4.00), produced phytohormone IAA and showed positive production of siderophores and inhibited growth of Fusarium culmorum to more than 50%. All differences between tested strains and control strain P. simiae WCS417 were also statistically confirmed. All strains showed positive results in monitoring plant growth promoting properties. The effect of three Bacillus strains on maize seeds in vivo conditions showed significant differences in root length (P<0.0001) and weight of the young plant (P<0.001) compared to control.\",\"PeriodicalId\":22746,\"journal\":{\"name\":\"The Journal of Microbiology, Biotechnology and Food Sciences\",\"volume\":\"24 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-03-23\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"5\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"The Journal of Microbiology, Biotechnology and Food Sciences\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.15414/JMBFS.3602\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"The Journal of Microbiology, Biotechnology and Food Sciences","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.15414/JMBFS.3602","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5

摘要

对植物活力有积极作用的细菌也被称为PGPB(植物生长促进细菌)。它们不仅存在于根区,而且存在于植物的地上部分,如内生细菌。本研究的目的是鉴定芽孢杆菌属细菌的促生长特性,并将其与拟假单胞菌WCS417(植物促生长菌株)进行比较。研究地点为Nitra附近Koliňany(40°26´46´N, 79°58´56´W),随机选取营养植物生长期BBCH 14-15的6株玉米(Zea mays L.)根系样品。对从植物根系中分离的细菌进行了鉴定,并对其生化参数进行了测试。从生化特征上观察了铁载体的检测、吲哚-3-乙酸(IAA)的测定、对磷酸盐的溶解能力和抗真菌活性的监测。将细菌悬浮液应用于玉米种子,并在体内控制条件下进行试验。分离菌株鉴定为弯曲芽孢杆菌、巨芽孢杆菌和枯草芽孢杆菌。3株菌株的磷酸盐增溶指数均达到中等水平(2.00≤SI: 4.00),产生植物激素IAA,产生铁载体,抑制镰刀菌生长50%以上。受试菌株与对照菌株类人猿疟原虫WCS417之间的差异也有统计学意义。所有菌株对植物生长促进性能的监测结果均为阳性。在体内条件下,3株芽孢杆菌对玉米种子的影响在根长(P<0.0001)和幼株重(P<0.001)上均显著高于对照。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
CHARACTERIZATION OF ENDOPHYTIC BACTERIA OF THE GENUS BACILLUS AND THEIR INFLUENCE ON THE GROWTH OF MAIZE (ZEA MAYS) IN VIVO
Bacteria with positive properties on plant vitality are also called PGPB (Plant Growth Promoting Bacteria). Their presence can be observed not only in the root area but also in the above-ground parts of plants like endophytic bacteria. The aim of our study was to characterize promoting features of bacteria from Bacillus genus and compare them with Pseudomonas simiae WCS417 (plant growth promoting strain). The work was carried out in locality Koliňany near Nitra (40°26´46´´N, 79°58´56´´W) and root samples were taken from 6 randomly selected plants of maize (Zea mays L.) in vegetative plant growth stage BBCH 14-15. Bacteria isolated from plant roots were identified and tested to biochemical parameters. From the biochemical features, we observed the detection of siderophores, determination of indole-3-acetic acid (IAA), monitoring the ability to dissolve phosphates and antifungal activity. Bacterial suspensions were applied to maize seeds and tested in vivo controlled conditions. Tested isolates were identified as Bacillus flexus, Bacillus megaterium and Bacillus subtilis. All 3 strains achieved the middle – class of phosphate solubilization index (2.00 ≤ SI ˂ 4.00), produced phytohormone IAA and showed positive production of siderophores and inhibited growth of Fusarium culmorum to more than 50%. All differences between tested strains and control strain P. simiae WCS417 were also statistically confirmed. All strains showed positive results in monitoring plant growth promoting properties. The effect of three Bacillus strains on maize seeds in vivo conditions showed significant differences in root length (P<0.0001) and weight of the young plant (P<0.001) compared to control.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信