EXTRACTION AND EVALUATION OF A NATURAL DYE FROM BISTORTA AMPLEXICAULE

Natural pigments extracted from plants are raised as a vital substitute to artificial dyes. The present study was conducted to extract pigment from Bistorta amplexicaule and evaluate its dyeing property, antimicrobial activity and toxicity. The extraction was done by maceration and sonication methods with 7.5% and 5% yield, respectively. The extracted pigment appeared bright- amber colored which was used for dyeing fabrics and food using traditional methods. The suitable dying was achieved using 4g dye powder in 100ml water for fabrics and 250mg in 26.2g of rice. The extract did not indicate antibacterial activity against Staphylococcus aureus, Klebsiella sp., Escherichia coli and Serratia marcescens by disc-diffusion method. Dye extract using albino mice assay did not show any mortality throughout the study period (15 days). The natural plant pigment extracted from B. amplexicaule had a potential as natural dye for food and fabric.