{"title":"探测器尺寸和荧光波长对三光子和双光子共聚焦显微镜分辨率的影响","authors":"Min Gu, X S Gan","doi":"10.1002/1361-6374(199609)4:3<129::AID-BIO3>3.0.CO;2-L","DOIUrl":null,"url":null,"abstract":"<p>Image formation in three- and two-photon confocal fluorescence scanning microscopy is analysed under the paraxial approximation. The analysis incorporates the effect of a finite-sized detector and the effect of the fluorescence wavelength. The resolution in the transverse and axial directions is examined for edge and layer objects, respectively. For a given excitation energy gap, three-photon confocal and non-confocal fluorescence microscopes feature a resolution close to that of their two-photon counterparts. However, the resolution of the three-photon microscopes is improved by up to 40-50% when the same excitation wavelength is used. This may prove advantageous in practical three-photon fluorescence microscopy.</p>","PeriodicalId":100176,"journal":{"name":"Bioimaging","volume":"4 3","pages":"129-137"},"PeriodicalIF":0.0000,"publicationDate":"1996-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/1361-6374(199609)4:3<129::AID-BIO3>3.0.CO;2-L","citationCount":"13","resultStr":"{\"title\":\"Effect of the detector size and the fluorescence wavelength on the resolution of three- and two-photon confocal microscopy\",\"authors\":\"Min Gu, X S Gan\",\"doi\":\"10.1002/1361-6374(199609)4:3<129::AID-BIO3>3.0.CO;2-L\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Image formation in three- and two-photon confocal fluorescence scanning microscopy is analysed under the paraxial approximation. The analysis incorporates the effect of a finite-sized detector and the effect of the fluorescence wavelength. The resolution in the transverse and axial directions is examined for edge and layer objects, respectively. For a given excitation energy gap, three-photon confocal and non-confocal fluorescence microscopes feature a resolution close to that of their two-photon counterparts. However, the resolution of the three-photon microscopes is improved by up to 40-50% when the same excitation wavelength is used. This may prove advantageous in practical three-photon fluorescence microscopy.</p>\",\"PeriodicalId\":100176,\"journal\":{\"name\":\"Bioimaging\",\"volume\":\"4 3\",\"pages\":\"129-137\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"1996-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1002/1361-6374(199609)4:3<129::AID-BIO3>3.0.CO;2-L\",\"citationCount\":\"13\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Bioimaging\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://onlinelibrary.wiley.com/doi/10.1002/1361-6374%28199609%294%3A3%3C129%3A%3AAID-BIO3%3E3.0.CO%3B2-L\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Bioimaging","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/1361-6374%28199609%294%3A3%3C129%3A%3AAID-BIO3%3E3.0.CO%3B2-L","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
Effect of the detector size and the fluorescence wavelength on the resolution of three- and two-photon confocal microscopy
Image formation in three- and two-photon confocal fluorescence scanning microscopy is analysed under the paraxial approximation. The analysis incorporates the effect of a finite-sized detector and the effect of the fluorescence wavelength. The resolution in the transverse and axial directions is examined for edge and layer objects, respectively. For a given excitation energy gap, three-photon confocal and non-confocal fluorescence microscopes feature a resolution close to that of their two-photon counterparts. However, the resolution of the three-photon microscopes is improved by up to 40-50% when the same excitation wavelength is used. This may prove advantageous in practical three-photon fluorescence microscopy.
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