Sejalben S. Chaudhari, Harshadkumar C. Chauhan, Kishan K. Sharma, Sandip S. Patel, Arun C. Patel, Sushil K. Mohapatra , Mehulkumar D. Srimali , Bharatsingh Chandel
{"title":"印度干热地区犬凝固酶阳性和凝固酶阴性葡萄球菌的耐药性模式","authors":"Sejalben S. Chaudhari, Harshadkumar C. Chauhan, Kishan K. Sharma, Sandip S. Patel, Arun C. Patel, Sushil K. Mohapatra , Mehulkumar D. Srimali , Bharatsingh Chandel","doi":"10.1016/j.tcam.2022.100679","DOIUrl":null,"url":null,"abstract":"<div><p><span>The emergence of antimicrobial resistance among </span><em>Staphylococcus</em> spp<em>.</em><span> isolated from clinical cases of canines<span> should be continuously monitored hence the present study was formulated to ascertain the antibiotypes and methicillin resistance in coagulase positive and coagulase negative staphylococci<span> of canine skin and associated mucous membrane affections from a hot and dry region of India. A total of 165 clinical samples were collected and </span></span></span><span><em>Staphylococcus aureus</em></span> was identified by conventional bacteriological methods and PCR. Antibiotic susceptibility test was done against commercially available antibiotic impregnated discs as per Clinical and Laboratory Standards Institute (CLSI) method. Methicillin resistance was determined by plate methods and then via PCR of <em>mecA</em> gene. These 165 samples yielded, 88 (53.33%) isolates of genus <em>Staphylococcus</em> and 46 <em>S. aureus</em><span> and 51/88 (57.95%) isolates were coagulase positive staphylococci<span>. Total 55 (62.5%) isolates showed susceptibility to Ceftriaxone/Sulbactum, 37 (42.05%) to Ciprofloxacin<span>, 26 (29.55%) to Oxacillin<span>, 24 (27.27%) to Penicillin, and 10 (11.36%) to Gentamicin. Total 21 methicillin-resistant </span></span></span></span><em>S. aureus</em> (MRSA) and 12 methicillin-resistant coagulase negative staphylococci (MRCoNS) were found on phenotypic basis whereas the <em>mecA</em> gene was detected in 6/21 MRSA and 2/12 MRCoNS isolates. <em>Staphylococcus</em> spp<em>.</em> showed increased level of resistance against commonly used antibiotics. The higher prevalence of methicillin resistance found with phenotypic methods than to <em>mecA</em> PCR indicates toward additional mechanisms responsible for emergence of MRS, especially in CoNS.</p></div>","PeriodicalId":23144,"journal":{"name":"Topics in companion animal medicine","volume":null,"pages":null},"PeriodicalIF":1.3000,"publicationDate":"2022-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":"{\"title\":\"Antibiotic Susceptibility Pattern of Canine Coagulase Positive and Coagulase Negative Staphylococcus spp. in a Hot and Dry Region of India\",\"authors\":\"Sejalben S. Chaudhari, Harshadkumar C. Chauhan, Kishan K. Sharma, Sandip S. Patel, Arun C. Patel, Sushil K. Mohapatra , Mehulkumar D. Srimali , Bharatsingh Chandel\",\"doi\":\"10.1016/j.tcam.2022.100679\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p><span>The emergence of antimicrobial resistance among </span><em>Staphylococcus</em> spp<em>.</em><span> isolated from clinical cases of canines<span> should be continuously monitored hence the present study was formulated to ascertain the antibiotypes and methicillin resistance in coagulase positive and coagulase negative staphylococci<span> of canine skin and associated mucous membrane affections from a hot and dry region of India. A total of 165 clinical samples were collected and </span></span></span><span><em>Staphylococcus aureus</em></span> was identified by conventional bacteriological methods and PCR. Antibiotic susceptibility test was done against commercially available antibiotic impregnated discs as per Clinical and Laboratory Standards Institute (CLSI) method. Methicillin resistance was determined by plate methods and then via PCR of <em>mecA</em> gene. These 165 samples yielded, 88 (53.33%) isolates of genus <em>Staphylococcus</em> and 46 <em>S. aureus</em><span> and 51/88 (57.95%) isolates were coagulase positive staphylococci<span>. Total 55 (62.5%) isolates showed susceptibility to Ceftriaxone/Sulbactum, 37 (42.05%) to Ciprofloxacin<span>, 26 (29.55%) to Oxacillin<span>, 24 (27.27%) to Penicillin, and 10 (11.36%) to Gentamicin. Total 21 methicillin-resistant </span></span></span></span><em>S. aureus</em> (MRSA) and 12 methicillin-resistant coagulase negative staphylococci (MRCoNS) were found on phenotypic basis whereas the <em>mecA</em> gene was detected in 6/21 MRSA and 2/12 MRCoNS isolates. <em>Staphylococcus</em> spp<em>.</em> showed increased level of resistance against commonly used antibiotics. The higher prevalence of methicillin resistance found with phenotypic methods than to <em>mecA</em> PCR indicates toward additional mechanisms responsible for emergence of MRS, especially in CoNS.</p></div>\",\"PeriodicalId\":23144,\"journal\":{\"name\":\"Topics in companion animal medicine\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2022-09-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"1\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Topics in companion animal medicine\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1938973622000526\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"VETERINARY SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Topics in companion animal medicine","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1938973622000526","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
Antibiotic Susceptibility Pattern of Canine Coagulase Positive and Coagulase Negative Staphylococcus spp. in a Hot and Dry Region of India
The emergence of antimicrobial resistance among Staphylococcus spp. isolated from clinical cases of canines should be continuously monitored hence the present study was formulated to ascertain the antibiotypes and methicillin resistance in coagulase positive and coagulase negative staphylococci of canine skin and associated mucous membrane affections from a hot and dry region of India. A total of 165 clinical samples were collected and Staphylococcus aureus was identified by conventional bacteriological methods and PCR. Antibiotic susceptibility test was done against commercially available antibiotic impregnated discs as per Clinical and Laboratory Standards Institute (CLSI) method. Methicillin resistance was determined by plate methods and then via PCR of mecA gene. These 165 samples yielded, 88 (53.33%) isolates of genus Staphylococcus and 46 S. aureus and 51/88 (57.95%) isolates were coagulase positive staphylococci. Total 55 (62.5%) isolates showed susceptibility to Ceftriaxone/Sulbactum, 37 (42.05%) to Ciprofloxacin, 26 (29.55%) to Oxacillin, 24 (27.27%) to Penicillin, and 10 (11.36%) to Gentamicin. Total 21 methicillin-resistant S. aureus (MRSA) and 12 methicillin-resistant coagulase negative staphylococci (MRCoNS) were found on phenotypic basis whereas the mecA gene was detected in 6/21 MRSA and 2/12 MRCoNS isolates. Staphylococcus spp. showed increased level of resistance against commonly used antibiotics. The higher prevalence of methicillin resistance found with phenotypic methods than to mecA PCR indicates toward additional mechanisms responsible for emergence of MRS, especially in CoNS.
期刊介绍:
Published quarterly, Topics in Companion Animal Medicine is a peer-reviewed veterinary scientific journal dedicated to providing practitioners with the most recent advances in companion animal medicine. The journal publishes high quality original clinical research focusing on important topics in companion animal medicine.