Gabriela Sabrina Anzollin , Lilian Zaki , Thalita Maria Perin , Bruna Finger , Letícia Trentin Perin , Fernanda Petry , Thalia Sebulsqui Saraiva , Maria Luiza Lima da Costa Lopes , Andresa Heemann Betti , Jaqueline Scapinello , J. Vladimir Oliveira , Jacir Dal Magro , Liz G. Müller
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To investigate the involvement of the monoaminergic system in the antidepressant-like activity of the extract, pharmacological antagonists were administered prior to the acute oral administration of the extract (60 mg/kg). Also, the interaction of the extract with antidepressants was assessed in the tail suspension test (TST). The <em>in vitro</em> inhibitory potential of <em>C. xanthocarpa</em> seeds extract towards MAO A and MAO B enzymes was tested <em>in vitro</em>.</p></div><div><h3>Results and conclusion</h3><p>Animals treated with <em>Campomanesia xanthocarpa</em> seeds extract showed a significant reduction in the immobility time in the TST. Mice pretreatment with SCH23390, sulpiride, prazosin, yohimbine, and <em>p</em>-chlorophenylalanine prevented the anti-immobility effect of the extract in the TST. The combined administration of sub-effective doses of the extract with imipramine, bupropion and fluoxetine significantly reduced mice immobility time in the TST. The extract showed MAO A inhibitory activity (IC<sub>50</sub> = 151.10 ± 5.75 μg/mL), which was greater than that toward MAO B (IC<sub>50</sub> > 400 μg/mL).</p><p>The extract of <em>Campomanesia xanthocarpa</em> seeds obtained by supercritical CO<sub>2</sub> shows antidepressant-like activity, which relies on the activation of the monoaminergic neurotransmission (serotoninergic, dopaminergic and noradrenergic), suggesting that this species might represent a resource for developing new antidepressants.</p></div>","PeriodicalId":17449,"journal":{"name":"Journal of Traditional and Complementary Medicine","volume":"12 4","pages":"Pages 309-317"},"PeriodicalIF":3.3000,"publicationDate":"2022-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.jtcme.2021.08.013","citationCount":"2","resultStr":"{\"title\":\"Antidepressant-like effect of Campomanesia xanthocarpa seeds in mice: Involvement of the monoaminergic system\",\"authors\":\"Gabriela Sabrina Anzollin , Lilian Zaki , Thalita Maria Perin , Bruna Finger , Letícia Trentin Perin , Fernanda Petry , Thalia Sebulsqui Saraiva , Maria Luiza Lima da Costa Lopes , Andresa Heemann Betti , Jaqueline Scapinello , J. 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The <em>in vitro</em> inhibitory potential of <em>C. xanthocarpa</em> seeds extract towards MAO A and MAO B enzymes was tested <em>in vitro</em>.</p></div><div><h3>Results and conclusion</h3><p>Animals treated with <em>Campomanesia xanthocarpa</em> seeds extract showed a significant reduction in the immobility time in the TST. Mice pretreatment with SCH23390, sulpiride, prazosin, yohimbine, and <em>p</em>-chlorophenylalanine prevented the anti-immobility effect of the extract in the TST. The combined administration of sub-effective doses of the extract with imipramine, bupropion and fluoxetine significantly reduced mice immobility time in the TST. 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Antidepressant-like effect of Campomanesia xanthocarpa seeds in mice: Involvement of the monoaminergic system
Background and aim
Campomanesia xanthocarpa Berg. (Myrtaceae) present several pharmacological actions, but there are no reports on its antidepressant-like potential. This study investigated the antidepressant-like effect and mechanism of action of Campomanesia xanthocarpa seeds extract obtained from supercritical CO2 (40 °C, 250 bar).
Experimental procedure
Mice were orally treated with the extract 1 h before the TST. To investigate the involvement of the monoaminergic system in the antidepressant-like activity of the extract, pharmacological antagonists were administered prior to the acute oral administration of the extract (60 mg/kg). Also, the interaction of the extract with antidepressants was assessed in the tail suspension test (TST). The in vitro inhibitory potential of C. xanthocarpa seeds extract towards MAO A and MAO B enzymes was tested in vitro.
Results and conclusion
Animals treated with Campomanesia xanthocarpa seeds extract showed a significant reduction in the immobility time in the TST. Mice pretreatment with SCH23390, sulpiride, prazosin, yohimbine, and p-chlorophenylalanine prevented the anti-immobility effect of the extract in the TST. The combined administration of sub-effective doses of the extract with imipramine, bupropion and fluoxetine significantly reduced mice immobility time in the TST. The extract showed MAO A inhibitory activity (IC50 = 151.10 ± 5.75 μg/mL), which was greater than that toward MAO B (IC50 > 400 μg/mL).
The extract of Campomanesia xanthocarpa seeds obtained by supercritical CO2 shows antidepressant-like activity, which relies on the activation of the monoaminergic neurotransmission (serotoninergic, dopaminergic and noradrenergic), suggesting that this species might represent a resource for developing new antidepressants.
期刊介绍:
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