黄颡鱼种子对小鼠的抗抑郁作用:单胺类系统的参与

IF 3.3 3区 医学 Q1 INTEGRATIVE & COMPLEMENTARY MEDICINE
Gabriela Sabrina Anzollin , Lilian Zaki , Thalita Maria Perin , Bruna Finger , Letícia Trentin Perin , Fernanda Petry , Thalia Sebulsqui Saraiva , Maria Luiza Lima da Costa Lopes , Andresa Heemann Betti , Jaqueline Scapinello , J. Vladimir Oliveira , Jacir Dal Magro , Liz G. Müller
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引用次数: 2

摘要

背景与目的黄果球蚧。(杨梅科)具有多种药理作用,但尚无关于其抗抑郁作用的报道。本研究研究了从超临界CO2(40°C,250 bar)中提取的黄果蜂种子提取物的抗抑郁作用和作用机制。实验程序:在TST前1小时用该提取物口服小鼠。为了研究单胺能系统参与提取物的抗抑郁样活性,在急性口服提取物(60mg/kg)之前给予药理学拮抗剂。此外,提取物与抗抑郁药的相互作用在尾悬试验(TST)中进行了评估。结果和结论:黄颡鱼种子提取物处理的动物在TST中的固定时间显著缩短。用SCH23390、舒必利、哌唑嗪、育亨宾和对氯苯丙氨酸预处理的小鼠阻止了提取物在TST中的抗固定作用。亚有效剂量的提取物与丙咪嗪、安非他酮和氟西汀联合给药显著减少了小鼠在TST中的不动时间。该提取物显示出对MAO A的抑制活性(IC50=151.10±5.75μg/mL),大于对MAO B的抑制作用(IC50>;400μg/mL,这表明该物种可能代表了开发新的抗抑郁药的资源。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Antidepressant-like effect of Campomanesia xanthocarpa seeds in mice: Involvement of the monoaminergic system

Antidepressant-like effect of Campomanesia xanthocarpa seeds in mice: Involvement of the monoaminergic system

Background and aim

Campomanesia xanthocarpa Berg. (Myrtaceae) present several pharmacological actions, but there are no reports on its antidepressant-like potential. This study investigated the antidepressant-like effect and mechanism of action of Campomanesia xanthocarpa seeds extract obtained from supercritical CO2 (40 °C, 250 bar).

Experimental procedure

Mice were orally treated with the extract 1 h before the TST. To investigate the involvement of the monoaminergic system in the antidepressant-like activity of the extract, pharmacological antagonists were administered prior to the acute oral administration of the extract (60 mg/kg). Also, the interaction of the extract with antidepressants was assessed in the tail suspension test (TST). The in vitro inhibitory potential of C. xanthocarpa seeds extract towards MAO A and MAO B enzymes was tested in vitro.

Results and conclusion

Animals treated with Campomanesia xanthocarpa seeds extract showed a significant reduction in the immobility time in the TST. Mice pretreatment with SCH23390, sulpiride, prazosin, yohimbine, and p-chlorophenylalanine prevented the anti-immobility effect of the extract in the TST. The combined administration of sub-effective doses of the extract with imipramine, bupropion and fluoxetine significantly reduced mice immobility time in the TST. The extract showed MAO A inhibitory activity (IC50 = 151.10 ± 5.75 μg/mL), which was greater than that toward MAO B (IC50 > 400 μg/mL).

The extract of Campomanesia xanthocarpa seeds obtained by supercritical CO2 shows antidepressant-like activity, which relies on the activation of the monoaminergic neurotransmission (serotoninergic, dopaminergic and noradrenergic), suggesting that this species might represent a resource for developing new antidepressants.

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来源期刊
Journal of Traditional and Complementary Medicine
Journal of Traditional and Complementary Medicine Medicine-Complementary and Alternative Medicine
CiteScore
9.30
自引率
6.70%
发文量
78
审稿时长
66 days
期刊介绍: eJTCM is committed to publish research providing the biological and clinical grounds for using Traditional and Complementary Medical treatments as well as studies that demonstrate the pathophysiological and molecular/biochemical bases supporting the effectiveness of such treatments. Review articles are by invitation only. eJTCM is receiving an increasing amount of submission, and we need to adopt more stringent criteria to select the articles that can be considered for peer review. Note that eJTCM is striving to increase the quality and medical relevance of the publications.
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