IL-27通过增强葡萄糖摄取和脂肪酸酯化来增加白色脂肪细胞的能量储存。

IF 3.5 4区 生物学 Q2 ENDOCRINOLOGY & METABOLISM
Adipocyte Pub Date : 2023-12-01 Epub Date: 2023-11-10 DOI:10.1080/21623945.2023.2276346
Chiara Scaffidi, Annie Srdic, Daniel Konrad, Stephan Wueest
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引用次数: 0

摘要

细胞因子白细胞介素(IL)-27已被报道可诱导白色脂肪细胞产热。然而,目前尚不清楚IL-27介导的脂肪细胞能量耗散是否与脂质和/或碳水化合物的能量供应增加平行。我们假设IL-27增加了白色脂肪细胞的脂解和葡萄糖摄取,从而为产热提供了底物。出乎意料的是,我们发现用IL-27处理3T3-L1脂肪细胞降低了细胞内和细胞外游离脂肪酸(FFA)浓度,并且激素敏感脂肪酶(HSL)的磷酸化不受IL-27的影响。这些结果在皮下白色脂肪细胞中得到证实。此外,将IL-27应用于3T3-L1脂肪细胞增加了细胞内甘油三酯(TG)含量,但没有增加线粒体ATP的产生,也没有增加参与β氧化的酶的表达,这表明升高的酯化而不是氧化导致FFA消失。此外,IL-27显著增加了GLUT1蛋白水平、基础葡萄糖摄取以及糖酵解ATP的产生,表明IL-27导致的糖酵解通量增加为TG合成提供了甘油骨架。总之,我们的研究结果表明IL-27增加了白色脂肪细胞的葡萄糖摄取和TG沉积。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
IL-27 increases energy storage in white adipocytes by enhancing glucose uptake and fatty acid esterification.

The cytokine interleukin (IL)-27 has been reported to induce thermogenesis in white adipocytes. However, it remains unknown whether IL-27-mediated adipocyte energy dissipation is paralleled by an elevated energy supply from lipids and/or carbohydrates. We hypothesized that IL-27 increases lipolysis and glucose uptake in white adipocytes, thereby providing substrates for thermogenesis. Unexpectedly, we found that treatment of 3T3-L1 adipocytes with IL-27 reduced intra- and extracellular free fatty acid (FFA) concentrations and that phosphorylation of hormone-sensitive lipase (HSL) was not affected by IL-27. These results were confirmed in subcutaneous white adipocytes. Further, application of IL-27 to 3T3-L1 adipocytes increased intracellular triglyceride (TG) content but not mitochondrial ATP production nor expression of enzymes involved in beta-oxidation indicating that elevated esterification rather than oxidation causes FFA disappearance. In addition, IL-27 significantly increased GLUT1 protein levels, basal glucose uptake as well as glycolytic ATP production, suggesting that increased glycolytic flux due to IL-27 provides the glycerol backbone for TG synthesis. In conclusion, our findings suggest IL-27 increases glucose uptake and TG deposition in white adipocytes.

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来源期刊
Adipocyte
Adipocyte Medicine-Histology
CiteScore
6.50
自引率
3.00%
发文量
46
审稿时长
32 weeks
期刊介绍: Adipocyte recognizes that the adipose tissue is the largest endocrine organ in the body, and explores the link between dysfunctional adipose tissue and the growing number of chronic diseases including diabetes, hypertension, cardiovascular disease and cancer. Historically, the primary function of the adipose tissue was limited to energy storage and thermoregulation. However, a plethora of research over the past 3 decades has recognized the dynamic role of the adipose tissue and its contribution to a variety of physiological processes including reproduction, angiogenesis, apoptosis, inflammation, blood pressure, coagulation, fibrinolysis, immunity and general metabolic homeostasis. The field of Adipose Tissue research has grown tremendously, and Adipocyte is the first international peer-reviewed journal of its kind providing a multi-disciplinary forum for research focusing exclusively on all aspects of adipose tissue physiology and pathophysiology. Adipocyte accepts high-profile submissions in basic, translational and clinical research.
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