Mohamed Abdo Rizk, Shimaa Abd El-Salam El-Sayed, Mohamed Z Sayed-Ahmed, Yosif Almoshari, Saad S Alqahtani, Sarfaraz Ahmad, Nawazish Alam, Basma H Marghani, Abdelbaset E Abdelbaset, Ikuo Igarashi
{"title":"辣木叶甲醇提取物对几种巴贝虫和马泰勒虫体外生长和小巴贝虫体内生长的抑制作用的评价。","authors":"Mohamed Abdo Rizk, Shimaa Abd El-Salam El-Sayed, Mohamed Z Sayed-Ahmed, Yosif Almoshari, Saad S Alqahtani, Sarfaraz Ahmad, Nawazish Alam, Basma H Marghani, Abdelbaset E Abdelbaset, Ikuo Igarashi","doi":"10.1155/2023/4285042","DOIUrl":null,"url":null,"abstract":"<p><p>The current study evaluated the inhibitory effect of <i>Moringa oleifera</i> leaves methanolic extract (MOL) against the <i>in vitro</i> growth of <i>Babesia bovis</i> (<i>B. bovis</i>), <i>B. caballi</i>, <i>B. bigemina</i>, and <i>Theileria equi</i> (<i>T. equi</i>), as well as <i>in vivo</i> growth of <i>B. microti</i> in mice. Active principles of MOL extract were determined using liquid chromatography mass spectrometry (LC-MS). MOL's anti-piroplasm efficacy was assessed both <i>in vitro</i> and <i>in vivo</i> using the SYBR Green I fluorescence assay. Every 96 hours, the hematological parameters, including red blood cell count (RBCs; 10<sup>4</sup>/UL), hemoglobin content (HGB; g/dl), and hematocrit percent (HCT; %), in the treated mice were monitored using a Celltac MEK6450 automated hematological analyzer. LC-MS of MOL revealed that the most abundant polyphenolic catechism found in the MOL extract was isoquercetin and rutin. MOL inhibited <i>B. bovis, B. caballi, B. bigemina</i>, and <i>T. equi in vitro</i> growth in a dose-dependent way, with IC<sub>50</sub> values of 45.29 ± 6.14, 19.16 ± 0.45, 137.49 ± 16.07, and 9.29 ± 0.014 <i>μ</i>g/ml, respectively. MOL's <i>in vitro</i> antibabesial activity was enhanced when administrated simultaneously with either diminazene aceturate (DA) or MMV665875 compound from malaria box. In mice infected by <i>B. microti</i>, a combination of MOL and a low dose of DA (12.5 mg·kg<sup>-1</sup>) resulted in a significant (<i>P</i> < 0.05) reduction in <i>B. microti</i> growth. These findings suggest that MOL is an effective herbal anti-piroplasm therapy, especially when combined with a low dosage of either DA or MMV665875.</p>","PeriodicalId":17527,"journal":{"name":"Journal of Tropical Medicine","volume":"2023 ","pages":"4285042"},"PeriodicalIF":2.1000,"publicationDate":"2023-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630014/pdf/","citationCount":"0","resultStr":"{\"title\":\"Evaluation of the Inhibitory Effect of <i>Moringa oleifera</i> Leaves Methanolic Extract against <i>In Vitro</i> Growth of Several <i>Babesia</i> Species and <i>Theileria equi</i> and the <i>In Vivo</i> Growth of <i>Babesia microti</i>.\",\"authors\":\"Mohamed Abdo Rizk, Shimaa Abd El-Salam El-Sayed, Mohamed Z Sayed-Ahmed, Yosif Almoshari, Saad S Alqahtani, Sarfaraz Ahmad, Nawazish Alam, Basma H Marghani, Abdelbaset E Abdelbaset, Ikuo Igarashi\",\"doi\":\"10.1155/2023/4285042\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>The current study evaluated the inhibitory effect of <i>Moringa oleifera</i> leaves methanolic extract (MOL) against the <i>in vitro</i> growth of <i>Babesia bovis</i> (<i>B. bovis</i>), <i>B. caballi</i>, <i>B. bigemina</i>, and <i>Theileria equi</i> (<i>T. equi</i>), as well as <i>in vivo</i> growth of <i>B. microti</i> in mice. Active principles of MOL extract were determined using liquid chromatography mass spectrometry (LC-MS). MOL's anti-piroplasm efficacy was assessed both <i>in vitro</i> and <i>in vivo</i> using the SYBR Green I fluorescence assay. Every 96 hours, the hematological parameters, including red blood cell count (RBCs; 10<sup>4</sup>/UL), hemoglobin content (HGB; g/dl), and hematocrit percent (HCT; %), in the treated mice were monitored using a Celltac MEK6450 automated hematological analyzer. LC-MS of MOL revealed that the most abundant polyphenolic catechism found in the MOL extract was isoquercetin and rutin. MOL inhibited <i>B. bovis, B. caballi, B. bigemina</i>, and <i>T. equi in vitro</i> growth in a dose-dependent way, with IC<sub>50</sub> values of 45.29 ± 6.14, 19.16 ± 0.45, 137.49 ± 16.07, and 9.29 ± 0.014 <i>μ</i>g/ml, respectively. MOL's <i>in vitro</i> antibabesial activity was enhanced when administrated simultaneously with either diminazene aceturate (DA) or MMV665875 compound from malaria box. In mice infected by <i>B. microti</i>, a combination of MOL and a low dose of DA (12.5 mg·kg<sup>-1</sup>) resulted in a significant (<i>P</i> < 0.05) reduction in <i>B. microti</i> growth. These findings suggest that MOL is an effective herbal anti-piroplasm therapy, especially when combined with a low dosage of either DA or MMV665875.</p>\",\"PeriodicalId\":17527,\"journal\":{\"name\":\"Journal of Tropical Medicine\",\"volume\":\"2023 \",\"pages\":\"4285042\"},\"PeriodicalIF\":2.1000,\"publicationDate\":\"2023-10-31\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10630014/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Tropical Medicine\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1155/2023/4285042\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/1/1 0:00:00\",\"PubModel\":\"eCollection\",\"JCR\":\"Q3\",\"JCRName\":\"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Tropical Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1155/2023/4285042","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/1/1 0:00:00","PubModel":"eCollection","JCR":"Q3","JCRName":"PUBLIC, ENVIRONMENTAL & OCCUPATIONAL HEALTH","Score":null,"Total":0}
Evaluation of the Inhibitory Effect of Moringa oleifera Leaves Methanolic Extract against In Vitro Growth of Several Babesia Species and Theileria equi and the In Vivo Growth of Babesia microti.
The current study evaluated the inhibitory effect of Moringa oleifera leaves methanolic extract (MOL) against the in vitro growth of Babesia bovis (B. bovis), B. caballi, B. bigemina, and Theileria equi (T. equi), as well as in vivo growth of B. microti in mice. Active principles of MOL extract were determined using liquid chromatography mass spectrometry (LC-MS). MOL's anti-piroplasm efficacy was assessed both in vitro and in vivo using the SYBR Green I fluorescence assay. Every 96 hours, the hematological parameters, including red blood cell count (RBCs; 104/UL), hemoglobin content (HGB; g/dl), and hematocrit percent (HCT; %), in the treated mice were monitored using a Celltac MEK6450 automated hematological analyzer. LC-MS of MOL revealed that the most abundant polyphenolic catechism found in the MOL extract was isoquercetin and rutin. MOL inhibited B. bovis, B. caballi, B. bigemina, and T. equi in vitro growth in a dose-dependent way, with IC50 values of 45.29 ± 6.14, 19.16 ± 0.45, 137.49 ± 16.07, and 9.29 ± 0.014 μg/ml, respectively. MOL's in vitro antibabesial activity was enhanced when administrated simultaneously with either diminazene aceturate (DA) or MMV665875 compound from malaria box. In mice infected by B. microti, a combination of MOL and a low dose of DA (12.5 mg·kg-1) resulted in a significant (P < 0.05) reduction in B. microti growth. These findings suggest that MOL is an effective herbal anti-piroplasm therapy, especially when combined with a low dosage of either DA or MMV665875.
期刊介绍:
Journal of Tropical Medicine is a peer-reviewed, Open Access journal that publishes original research articles, review articles, and clinical studies on all aspects of tropical diseases. Articles on the pathology, diagnosis, and treatment of tropical diseases, parasites and their hosts, epidemiology, and public health issues will be considered. Journal of Tropical Medicine aims to facilitate the communication of advances addressing global health and mortality relating to tropical diseases.