{"title":"伊朗东南部克尔曼市尿路致病性大肠杆菌分离株中整合子、超广谱β-内酰胺酶和遗传多样性的特征。","authors":"Samane Mohebi, Zahra Golestani-Hotkani, Maryam Foulad-Pour, Peivand Nazeri, Fahimeh Mohseni, Zahra Hashemizadeh, Zahra Moghani-Bashi, Naser Niksefat, Sanaz Rastegar, Maryam Khajedadian, Zahra Lotfian, Hossein Hosseini-Nave","doi":"10.18502/ijm.v15i5.13867","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong>The study aimed to investigate the distribution of genes encoding integrons, extended spectrum beta-lactamase (ESBL) in <i>E. coli</i> isolated from UTIs, as well as the genetic diversity among the isolates.</p><p><strong>Materials and methods: </strong><i>E. coli</i> isolates were recovered from the patients with UTI in Kerman Iran. Antibiotic susceptibility was done according to CLSI guidelines. The presence of ESBL genes and integrons was evaluated using PCR. PCR and sequencing were applied for the evaluation of cassette content of integrons. Genotyping of the isolates was performed by multiple-locus variable-number tandem repeat analysis (MLVA).</p><p><strong>Results: </strong>Imipenem was the most effective antibiotic, while the highest resistance was observed to streptomycin. In total 40.2% of isolates were ESBL producers. Of 69 integron-positive isolates, 59 only had class I integrons, 4 only had class II integrons and 6 had both types. The most common gene cassette found within class I integrons was <i>dfrA17-aadA5</i> (n=27). The <i>E. coli</i> isolates were divided into 16 MLVA clusters.</p><p><strong>Conclusion: </strong>The current study demonstrated the simultaneous presence of class I integrons and ESBLs involved in the resistance of UPEC isolates to antibacterial agents. Our finding also revealed that the <i>E. coli</i> isolates belonged to diverse clones.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"15 5","pages":"616-624"},"PeriodicalIF":1.3000,"publicationDate":"2023-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10628077/pdf/","citationCount":"0","resultStr":"{\"title\":\"Characterization of integrons, extended spectrum beta lactamases and genetic diversity among uropathogenic <i>Escherichia coli</i> isolates from Kerman, south east of Iran.\",\"authors\":\"Samane Mohebi, Zahra Golestani-Hotkani, Maryam Foulad-Pour, Peivand Nazeri, Fahimeh Mohseni, Zahra Hashemizadeh, Zahra Moghani-Bashi, Naser Niksefat, Sanaz Rastegar, Maryam Khajedadian, Zahra Lotfian, Hossein Hosseini-Nave\",\"doi\":\"10.18502/ijm.v15i5.13867\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background and objectives: </strong>The study aimed to investigate the distribution of genes encoding integrons, extended spectrum beta-lactamase (ESBL) in <i>E. coli</i> isolated from UTIs, as well as the genetic diversity among the isolates.</p><p><strong>Materials and methods: </strong><i>E. coli</i> isolates were recovered from the patients with UTI in Kerman Iran. Antibiotic susceptibility was done according to CLSI guidelines. The presence of ESBL genes and integrons was evaluated using PCR. PCR and sequencing were applied for the evaluation of cassette content of integrons. Genotyping of the isolates was performed by multiple-locus variable-number tandem repeat analysis (MLVA).</p><p><strong>Results: </strong>Imipenem was the most effective antibiotic, while the highest resistance was observed to streptomycin. In total 40.2% of isolates were ESBL producers. Of 69 integron-positive isolates, 59 only had class I integrons, 4 only had class II integrons and 6 had both types. The most common gene cassette found within class I integrons was <i>dfrA17-aadA5</i> (n=27). The <i>E. coli</i> isolates were divided into 16 MLVA clusters.</p><p><strong>Conclusion: </strong>The current study demonstrated the simultaneous presence of class I integrons and ESBLs involved in the resistance of UPEC isolates to antibacterial agents. Our finding also revealed that the <i>E. coli</i> isolates belonged to diverse clones.</p>\",\"PeriodicalId\":14633,\"journal\":{\"name\":\"Iranian Journal of Microbiology\",\"volume\":\"15 5\",\"pages\":\"616-624\"},\"PeriodicalIF\":1.3000,\"publicationDate\":\"2023-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10628077/pdf/\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Microbiology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.18502/ijm.v15i5.13867\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v15i5.13867","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Characterization of integrons, extended spectrum beta lactamases and genetic diversity among uropathogenic Escherichia coli isolates from Kerman, south east of Iran.
Background and objectives: The study aimed to investigate the distribution of genes encoding integrons, extended spectrum beta-lactamase (ESBL) in E. coli isolated from UTIs, as well as the genetic diversity among the isolates.
Materials and methods: E. coli isolates were recovered from the patients with UTI in Kerman Iran. Antibiotic susceptibility was done according to CLSI guidelines. The presence of ESBL genes and integrons was evaluated using PCR. PCR and sequencing were applied for the evaluation of cassette content of integrons. Genotyping of the isolates was performed by multiple-locus variable-number tandem repeat analysis (MLVA).
Results: Imipenem was the most effective antibiotic, while the highest resistance was observed to streptomycin. In total 40.2% of isolates were ESBL producers. Of 69 integron-positive isolates, 59 only had class I integrons, 4 only had class II integrons and 6 had both types. The most common gene cassette found within class I integrons was dfrA17-aadA5 (n=27). The E. coli isolates were divided into 16 MLVA clusters.
Conclusion: The current study demonstrated the simultaneous presence of class I integrons and ESBLs involved in the resistance of UPEC isolates to antibacterial agents. Our finding also revealed that the E. coli isolates belonged to diverse clones.
期刊介绍：
The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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