小牛实验性共同感染SARS-CoV-2德尔塔和奥密克戎变异毒株。

IF 8.4 2区 医学 Q1 IMMUNOLOGY
Emerging Microbes & Infections Pub Date : 2024-12-01 Epub Date: 2023-12-30 DOI:10.1080/22221751.2023.2281356
Konner Cool, Natasha N Gaudreault, Jessie D Trujillo, Igor Morozov, Chester D McDowell, Dashzeveg Bold, Taeyong Kwon, Velmurugan Balaraman, Patricia Assato, Daniel W Madden, Emily Mantlo, Jayme Souza-Neto, Franco Matias-Ferreyra, Jaime Retallick, Gagandeep Singh, Michael Schotsaert, Mariano Carossino, Udeni B R Balasuriya, William C Wilson, Roman M Pogranichniy, Adolfo García-Sastre, Juergen A Richt
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引用次数: 0

摘要

自2019年末出现以来,严重急性呼吸综合征冠状病毒2型(严重急性呼吸系统综合征冠状病毒冠状病毒2型)已多次跨越物种屏障,在各种家畜和野生动物物种中报告了自然感染。SARS-CoV-2变异毒株(VOCs)的出现和全球传播扩大了易感宿主物种的范围。先前使用武汉型严重急性呼吸系统综合征冠状病毒2型分离株对牛进行的实验性感染研究表明,牛不太可能扩增严重急性呼吸系综合征冠状病毒的宿主。然而,严重急性呼吸系统综合征冠状病毒2型血清和RNA阳性的牛已经在欧洲、印度和非洲被发现。在这里,我们调查了德尔塔和奥密克戎严重急性呼吸系统综合征冠状病毒2型挥发性有机物在牛中的易感性和传播。用混合接种的严重急性呼吸系统综合征冠状病毒2型挥发性有机物德尔塔和奥密克戎BA.2对8头荷斯坦小牛进行了口服和鼻内联合感染。在攻击后24小时,引入两只哨点小牛来评估病毒传播。共同感染导致小牛在攻击后1天和2天(DPC)脱落严重急性呼吸系统综合征冠状病毒2型RNA的比例很高。在3和7 DPC时观察到严重急性呼吸系统综合征冠状病毒2型RNA的广泛组织分布,在3 DPC时从两只小牛身上回收了感染性病毒。下一代测序显示,在临床样本或组织中只检测到严重急性呼吸系统综合征冠状病毒2型德尔塔变异株。与之前在牛身上进行的实验性感染研究类似,我们只观察到有限的血清转化,没有明确的证据表明传播给哨点小牛。总之,我们的研究结果表明,与奥密克戎BA.2和武汉样分离株相比,牛更容易感染严重急性呼吸系统综合征冠状病毒2型德尔塔毒株,但在没有水平传播的情况下,不太可能成为目前流行的严重急性呼吸系统冠状病毒2型变异株的宿主。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Experimental co-infection of calves with SARS-CoV-2 Delta and Omicron variants of concern.

Since emerging in late 2019, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has repeatedly crossed the species barrier with natural infections reported in various domestic and wild animal species. The emergence and global spread of SARS-CoV-2 variants of concern (VOCs) has expanded the range of susceptible host species. Previous experimental infection studies in cattle using Wuhan-like SARS-CoV-2 isolates suggested that cattle were not likely amplifying hosts for SARS-CoV-2. However, SARS-CoV-2 sero- and RNA-positive cattle have since been identified in Europe, India, and Africa. Here, we investigated the susceptibility and transmission of the Delta and Omicron SARS-CoV-2 VOCs in cattle. Eight Holstein calves were co-infected orally and intranasally with a mixed inoculum of SARS-CoV-2 VOCs Delta and Omicron BA.2. Twenty-four hours post-challenge, two sentinel calves were introduced to evaluate virus transmission. The co-infection resulted in a high proportion of calves shedding SARS-CoV-2 RNA at 1- and 2-days post-challenge (DPC). Extensive tissue distribution of SARS-CoV-2 RNA was observed at 3 and 7 DPC and infectious virus was recovered from two calves at 3 DPC. Next-generation sequencing revealed that only the SARS-CoV-2 Delta variant was detected in clinical samples and tissues. Similar to previous experimental infection studies in cattle, we observed only limited seroconversion and no clear evidence of transmission to sentinel calves. Together, our findings suggest that cattle are more permissive to infection with SARS-CoV-2 Delta than Omicron BA.2 and Wuhan-like isolates but, in the absence of horizontal transmission, are not likely to be reservoir hosts for currently circulating SARS-CoV-2 variants.

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来源期刊
Emerging Microbes & Infections
Emerging Microbes & Infections IMMUNOLOGY-MICROBIOLOGY
CiteScore
26.20
自引率
2.30%
发文量
276
审稿时长
20 weeks
期刊介绍: Emerging Microbes & Infections is a peer-reviewed, open-access journal dedicated to publishing research at the intersection of emerging immunology and microbiology viruses. The journal's mission is to share information on microbes and infections, particularly those gaining significance in both biological and clinical realms due to increased pathogenic frequency. Emerging Microbes & Infections is committed to bridging the scientific gap between developed and developing countries. This journal addresses topics of critical biological and clinical importance, including but not limited to: - Epidemic surveillance - Clinical manifestations - Diagnosis and management - Cellular and molecular pathogenesis - Innate and acquired immune responses between emerging microbes and their hosts - Drug discovery - Vaccine development research Emerging Microbes & Infections invites submissions of original research articles, review articles, letters, and commentaries, fostering a platform for the dissemination of impactful research in the field.
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