Mete Ahlat , Cumhur Aydin , Sinem Kaya , Mehmet Baysallar
{"title":"使用基质辅助激光解吸/电离飞行时间质谱仪鉴定根尖周组织疾病的根管微生物群图谱。","authors":"Mete Ahlat , Cumhur Aydin , Sinem Kaya , Mehmet Baysallar","doi":"10.1016/j.anaerobe.2023.102791","DOIUrl":null,"url":null,"abstract":"<div><h3>Objectives</h3><p>The purpose of this study was to identify microorganisms isolated from various periapical tissue diseases using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) and classify them via an unsupervised machine learning approach.</p></div><div><h3>Methods</h3><p>A total of 150 patients with various apical conditions and teeth in need of endodontic retreatment were divided into five groups, including Retreatment, Acute Apical Abscess, Chronic Apical Abscess, Acute Apical Periodontitis, and Chronic Apical Periodontitis. Samples were collected from root canals using paper points after agitating with a #10 K file then microorganisms were identified using MALDI-TOF-MS. Data were analyzed using a hierarchical clustering method. Quadruple clusters and dendrograms were formed according to similarities and dissimilarities.</p></div><div><h3>Results</h3><p>A total of 80 species were identified under six different phyla. The most similar microorganism species were identified <em>''Enterococcus faecalis''</em> between 21 and 23-year-old female cases in Retreatment group; <em>''Lactobacillus rhamnosus''</em> between 20 and 18-year-old male cases in Symptomatic Apical Abscess cases; <em>''Lactobacillus paracasei''</em> between 26 and 40-year-old male cases in Asymptomatic Apical Abscess cases; <em>''Enterococcus faecalis''</em> between 48 and 50-year-old female cases in Symptomatic Apical Periodontitis cases; <em>''Lactobacillus rhamnosus''</em> between 48 and 60-year-old male cases in Asymptomatic Apical Periodontitis cases.</p></div><div><h3>Conclusions</h3><p>MALDI-TOF MS can be considered a fast and high-throughput screening technique for microbial species identification in endodontics. Thus, it will provide valuable data for future research designs regarding periapical tissue diseases. As the MALDI-TOF MS database expands and comprehensive data becomes available, the relationship between microbial profiles and disease progression will become increasingly apparent.</p></div>","PeriodicalId":8050,"journal":{"name":"Anaerobe","volume":"84 ","pages":"Article 102791"},"PeriodicalIF":2.5000,"publicationDate":"2023-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification of root canal microbiota profiles of periapical tissue diseases using matrix-assisted laser desorption/ionization time-of-flight mass spectrometer\",\"authors\":\"Mete Ahlat , Cumhur Aydin , Sinem Kaya , Mehmet Baysallar\",\"doi\":\"10.1016/j.anaerobe.2023.102791\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><h3>Objectives</h3><p>The purpose of this study was to identify microorganisms isolated from various periapical tissue diseases using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) and classify them via an unsupervised machine learning approach.</p></div><div><h3>Methods</h3><p>A total of 150 patients with various apical conditions and teeth in need of endodontic retreatment were divided into five groups, including Retreatment, Acute Apical Abscess, Chronic Apical Abscess, Acute Apical Periodontitis, and Chronic Apical Periodontitis. Samples were collected from root canals using paper points after agitating with a #10 K file then microorganisms were identified using MALDI-TOF-MS. Data were analyzed using a hierarchical clustering method. Quadruple clusters and dendrograms were formed according to similarities and dissimilarities.</p></div><div><h3>Results</h3><p>A total of 80 species were identified under six different phyla. The most similar microorganism species were identified <em>''Enterococcus faecalis''</em> between 21 and 23-year-old female cases in Retreatment group; <em>''Lactobacillus rhamnosus''</em> between 20 and 18-year-old male cases in Symptomatic Apical Abscess cases; <em>''Lactobacillus paracasei''</em> between 26 and 40-year-old male cases in Asymptomatic Apical Abscess cases; <em>''Enterococcus faecalis''</em> between 48 and 50-year-old female cases in Symptomatic Apical Periodontitis cases; <em>''Lactobacillus rhamnosus''</em> between 48 and 60-year-old male cases in Asymptomatic Apical Periodontitis cases.</p></div><div><h3>Conclusions</h3><p>MALDI-TOF MS can be considered a fast and high-throughput screening technique for microbial species identification in endodontics. Thus, it will provide valuable data for future research designs regarding periapical tissue diseases. As the MALDI-TOF MS database expands and comprehensive data becomes available, the relationship between microbial profiles and disease progression will become increasingly apparent.</p></div>\",\"PeriodicalId\":8050,\"journal\":{\"name\":\"Anaerobe\",\"volume\":\"84 \",\"pages\":\"Article 102791\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2023-11-02\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Anaerobe\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S1075996423001002\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Anaerobe","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1075996423001002","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Identification of root canal microbiota profiles of periapical tissue diseases using matrix-assisted laser desorption/ionization time-of-flight mass spectrometer
Objectives
The purpose of this study was to identify microorganisms isolated from various periapical tissue diseases using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF-MS) and classify them via an unsupervised machine learning approach.
Methods
A total of 150 patients with various apical conditions and teeth in need of endodontic retreatment were divided into five groups, including Retreatment, Acute Apical Abscess, Chronic Apical Abscess, Acute Apical Periodontitis, and Chronic Apical Periodontitis. Samples were collected from root canals using paper points after agitating with a #10 K file then microorganisms were identified using MALDI-TOF-MS. Data were analyzed using a hierarchical clustering method. Quadruple clusters and dendrograms were formed according to similarities and dissimilarities.
Results
A total of 80 species were identified under six different phyla. The most similar microorganism species were identified ''Enterococcus faecalis'' between 21 and 23-year-old female cases in Retreatment group; ''Lactobacillus rhamnosus'' between 20 and 18-year-old male cases in Symptomatic Apical Abscess cases; ''Lactobacillus paracasei'' between 26 and 40-year-old male cases in Asymptomatic Apical Abscess cases; ''Enterococcus faecalis'' between 48 and 50-year-old female cases in Symptomatic Apical Periodontitis cases; ''Lactobacillus rhamnosus'' between 48 and 60-year-old male cases in Asymptomatic Apical Periodontitis cases.
Conclusions
MALDI-TOF MS can be considered a fast and high-throughput screening technique for microbial species identification in endodontics. Thus, it will provide valuable data for future research designs regarding periapical tissue diseases. As the MALDI-TOF MS database expands and comprehensive data becomes available, the relationship between microbial profiles and disease progression will become increasingly apparent.
期刊介绍：
Anaerobe is essential reading for those who wish to remain at the forefront of discoveries relating to life processes of strictly anaerobes. The journal is multi-disciplinary, and provides a unique forum for those investigating anaerobic organisms that cause infections in humans and animals, as well as anaerobes that play roles in microbiomes or environmental processes.
Anaerobe publishes reviews, mini reviews, original research articles, notes and case reports. Relevant topics fall into the broad categories of anaerobes in human and animal diseases, anaerobes in the microbiome, anaerobes in the environment, diagnosis of anaerobes in clinical microbiology laboratories, molecular biology, genetics, pathogenesis, toxins and antibiotic susceptibility of anaerobic bacteria.
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