应用基于聚二甲基丙烯酰胺的β-内酰胺类抗生素和β-内酶抑制剂表面功能化的高效β-内内酰胺酶测定。

IF 5.4 2区 工程技术 Q1 BIOCHEMICAL RESEARCH METHODS
Lab on a Chip Pub Date : 2023-11-02 DOI:10.1039/D3LC00682D
Simone Rentschler, Max Borgolte, Alexander Filbert, Stefan Laufer and Hans-Peter Deigner
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引用次数: 0

摘要

近几十年来,β-内酰胺酶的兴起极大地导致了抗生素耐药性的出现和广泛传播,对全球健康构成了严重威胁。人们越来越需要开发快速、成本效益高、用户友好的诊断检测方法,以准确检测β-内酰胺酶,从而优化患者的预后并防止多药耐药性的传播。在本文中,我们提出了一种基于聚二甲基丙烯酰胺(PDMA)的表面功能化,以固定不同亚类的β-内酰胺类抗生素和β-内酶抑制剂。通过C,H插入反应通过UV交联诱导固定化。该功能涂层成功应用于高效检测重组β-内酰胺酶以及从临床相关菌株中分离的β-内糖苷酶。因此,该方法描述了一种创新的方法,对诊断应用具有几个显著的好处:创建针对β-内酰胺酶活性的特异性检测平台,开发高通量诊断分析以及稳定性和保质期方面的好处。此外,该方法高度适用于其他表面、抗生素和分析物,对各种生物医学、环境和抗菌应用具有深远的影响。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Highly efficient β-lactamase assay applying poly-dimethylacrylamide-based surface functionalization with β-lactam antibiotics and β-lactamase inhibitors†

Highly efficient β-lactamase assay applying poly-dimethylacrylamide-based surface functionalization with β-lactam antibiotics and β-lactamase inhibitors†

In recent decades, the rise of β-lactamases has substantially led to the emergence and wide spread of antibiotic resistance posing a serious global health threat. There is growing need for the development of rapid, cost-effective and user-friendly diagnostic assays for the accurate detection of β-lactamases to optimize patient outcomes and prevent the spread of multidrug-resistances. In this article, we present a poly-dimethylacrylamide (PDMA)-based surface functionalization to immobilize β-lactam antibiotics and β-lactamase inhibitors of different subclasses. Immobilization was induced via UV-crosslinking through C,H-insertion reactions. The functional coatings were successfully applied in a highly efficient assay for the determination of recombinant β-lactamases as well as β-lactamases isolated from clinically relevant bacterial strains. Thus, this method describes an innovative approach with several significant benefits for diagnostic applications: the creation of specific detection platforms tailored for β-lactamase activity, the development of high-throughput diagnostic assays and benefits regarding stability and shelf-life. Furthermore, this method is highly adaptable to other surfaces, antibiotics, and analytes, offering far-reaching implications for various biomedical, environmental, and antimicrobial applications.

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来源期刊
Lab on a Chip
Lab on a Chip 工程技术-化学综合
CiteScore
11.10
自引率
8.20%
发文量
434
审稿时长
2.6 months
期刊介绍: Lab on a Chip is the premiere journal that publishes cutting-edge research in the field of miniaturization. By their very nature, microfluidic/nanofluidic/miniaturized systems are at the intersection of disciplines, spanning fundamental research to high-end application, which is reflected by the broad readership of the journal. Lab on a Chip publishes two types of papers on original research: full-length research papers and communications. Papers should demonstrate innovations, which can come from technical advancements or applications addressing pressing needs in globally important areas. The journal also publishes Comments, Reviews, and Perspectives.
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