TNFSF15促进肿瘤中CD11b+髓系细胞分化为血管周细胞。

IF 5.6 2区 医学 Q1 MEDICINE, RESEARCH & EXPERIMENTAL
Xiangxiang Gu, Yipan Zhu, Cancan Zhao, Yixin Cao, Jingying Wang, Qiangzhe Zhang, Luyuan Li
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引用次数: 0

摘要

目的:缺乏周细胞覆盖的未成熟血管系统显著促进肿瘤生长、耐药性和癌症细胞扩散。我们先前证明肿瘤坏死因子超家族15(TNFSF15)是一种在调节造血和血管稳态中发挥重要作用的细胞因子。本研究的主要目的是探讨TNFSF15是否可以促进新分离的髓系细胞分化为CD11b+细胞并进一步分化为周细胞。方法:用红色荧光骨髓建立癌症小鼠模型。TNFSF15治疗后,评估肿瘤中CD11b+骨髓细胞和血管周细胞,以及周细胞和血管内皮细胞的共同定位。此外,从野生型小鼠中分离CD11b+细胞,并用TNFSF15处理以确定对这些细胞分化的影响。结果:在TNFSF15治疗的肿瘤中,我们观察到骨髓来源的CD11b+髓细胞和血管周细胞的百分比升高,后者与血管内皮细胞共定位。TNFSF15通过下调Wnt3a-EGFR1和上调CD49e-FN信号通路,保护CD11b+细胞免于凋亡,并促进这些细胞分化为周细胞。结论:TNFSF15促进骨髓中CD11b+细胞的产生,并促进这些细胞分化为周细胞,这可能稳定肿瘤新血管系统。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
TNFSF15 facilitates the differentiation of CD11b+ myeloid cells into vascular pericytes in tumors.

Objective: Immature vasculature lacking pericyte coverage substantially contributes to tumor growth, drug resistance, and cancer cell dissemination. We previously demonstrated that tumor necrosis factor superfamily 15 (TNFSF15) is a cytokine with important roles in modulating hematopoiesis and vascular homeostasis. The main purpose of this study was to explore whether TNFSF15 might promote freshly isolated myeloid cells to differentiate into CD11b+ cells and further into pericytes.

Methods: A model of Lewis lung cancer was established in mice with red fluorescent bone marrow. After TNFSF15 treatment, CD11b+ myeloid cells and vascular pericytes in the tumors, and the co-localization of pericytes and vascular endothelial cells, were assessed. Additionally, CD11b+ cells were isolated from wild-type mice and treated with TNFSF15 to determine the effects on the differentiation of these cells.

Results: We observed elevated percentages of bone marrow-derived CD11b+ myeloid cells and vascular pericytes in TNFSF15-treated tumors, and the latter cells co-localized with vascular endothelial cells. TNFSF15 protected against CD11b+ cell apoptosis and facilitated the differentiation of these cells into pericytes by down-regulating Wnt3a-VEGFR1 and up-regulating CD49e-FN signaling pathways.

Conclusions: TNFSF15 facilitates the production of CD11b+ cells in the bone marrow and promotes the differentiation of these cells into pericytes, which may stabilize the tumor neovasculature.

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来源期刊
Cancer Biology & Medicine
Cancer Biology & Medicine Medicine-Oncology
CiteScore
9.80
自引率
3.60%
发文量
1143
审稿时长
12 weeks
期刊介绍: Cancer Biology & Medicine (ISSN 2095-3941) is a peer-reviewed open-access journal of Chinese Anti-cancer Association (CACA), which is the leading professional society of oncology in China. The journal quarterly provides innovative and significant information on biological basis of cancer, cancer microenvironment, translational cancer research, and all aspects of clinical cancer research. The journal also publishes significant perspectives on indigenous cancer types in China.
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