应用生物信息学和综合分析研究阿尔茨海默病患者血浆外泌体环状核糖核酸-微核糖核酸表达谱。

IF 0.9 Q3 MEDICINE, GENERAL & INTERNAL
Nail Besli, Bahar Sarikamis, Rabia Kalkan Cakmak, Ulkan Kilic
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引用次数: 0

摘要

目的:阿尔茨海默病是一种神经退行性疾病,在世界范围内随着年龄的增长而增加。大量证据表明,外泌体非编码核糖核酸在阿尔茨海默病发展中的作用,但这些非编码核糖酸介导的调节机制仍然广泛未知。本研究利用阿尔茨海默病患者的血浆样本,探索了外泌体环状核糖核酸-微小核糖核酸图谱。材料和方法:使用ArrayExpress平台传输每组3个样本(健康、轻度认知障碍和阿尔茨海默病)的数据。使用血浆外泌体,比较阿尔茨海默病和轻度认知障碍组之间差异表达的微小核糖核酸和差异表达的环状核糖核酸。然后,为了定义通路、基因本体和网络,分析了轻度认知障碍和阿尔茨海默病组常见的差异表达的微小核糖核酸和差异表达的环状核糖核酸。最后,对枢纽基因的选择和蛋白质-蛋白质相互作用网络进行了分析。结果:共识别出19种常见的差异表达的微小核糖核酸(7种上调,12种下调)和24种差异表达的环状核糖核酸。共有4559个靶基因被预测为上调的差异表达的微小核糖核酸,而6504个靶基因则被鉴定为下调的差异表达微小核糖核酸。大多数靶基因参与磷酸肌醇3-激酶-Akt途径,主要由hsa-mir-374a-3p、mir-196a-5p、let-205-5p、mir-185-3p、mir-374a-5p调节,mir-615-3p,let-7c-5p,mir-185-5p。此外,通过使用Cytoscape软件中的cytohubba的蛋白质-蛋白质相互作用网络,9个枢纽基因(HSP90AA、ACTB、MAPK1、GSK3B、CCNE2、CDK6、AKT1、IGF1R、CCND1)被揭示为与阿尔茨海默病显著相关的基因。结论:我们的发现为微小核糖核酸与环状核糖核酸在阿尔茨海默病发病机制中的联系提供了一个新的视角。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Exosomal Circular Ribonucleic Acid-Microribonucleic Acid Expression Profile from Plasma in Alzheimer's Disease Patients by Bioinformatics and Integrative Analysis.

Objective: Alzheimer's disease is a neurodegenerative sickness and increasing with age throughout the world. A substantial body of evidence suggests the role of exosomal noncoding ribonucleic acids in the development of Alzheimer's disease, but the regulatory mechanisms mediated by these noncoding ribonucleic acids remain extensively unknown. Using plasma samples from Alzheimer's disease patients, this study explored the exosomal circular ribonucleic acid-microribonucleic acid profiles.

Materials and methods: The ArrayExpress platform was used to convey data from 3 samples from each group (healthy, mild cognitive impairment, and Alzheimer's disease). Using plasma exosomes, differentially expressed microribonucleic acids and differentially expressed circular ribonucleic acids were compared between the Alzheimer's disease and mild cognitive impairment groups. Afterward, to define pathways, gene ontologies, and networks, differentially expressed microribonucleic acids and differentially expressed circular ribonucleic acids common to both mild cognitive impairment and Alzheimer's disease groups were analyzed. Eventually, the selection of hub genes and protein-protein interaction network was analyzed.

Results: A total of common 19 (7 upregulated and 12 downregulated) differentially expressed microribonucleic acids and 24 differentially expressed circular ribonucleic acids were recognized. A total of 4559 target genes were predicted for upregulated differentially expressed microribonucleic acids, while 6504 target genes were identified for downregulated differentially expressed microribonucleic acids, and most of the target genes involved in the phosphoinositide 3-kinases-Akt pathway and that were mostly regulated by hsa-mir-374a-3p, mir-196a-5p, let-205-5p, mir-185-3p, mir-374a-5p, mir-615-3p, let-7c-5p, mir-185-5p. Additionally, 9 hub genes (HSP90AA, ACTB, MAPK1, GSK3B, CCNE2, CDK6, AKT1, IGF1R, CCND1) were revealed as the genes considerably related to Alzheimer's disease by a protein-protein interaction network using the cytohubba in Cytoscape software.

Conclusion: Our findings provide a new perspective on how microribonucleic acids could connect with circular ribonucleic acids in the pathogenesis of Alzheimer's disease.

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来源期刊
Eurasian Journal of Medicine
Eurasian Journal of Medicine Medicine-Medicine (all)
CiteScore
1.90
自引率
6.70%
发文量
59
审稿时长
16 weeks
期刊介绍: Eurasian Journal of Medicine (Eurasian J Med) is an international, scientific, open access periodical published by independent, unbiased, and triple-blinded peer-review principles. The journal is the official publication of Atatürk University School of Medicine and published triannually in February, June, and October. The publication language of the journal is English. The aim of the Eurasian Journal of Medicine is to publish original research papers of the highest scientific and clinical value in all medical fields. The Eurasian J Med also includes reviews, editorial short notes and letters to the editor that either as a comment related to recently published articles in our journal or as a case report. The target audience of the journal includes researchers, physicians and healthcare professionals who are interested or working in in all medical disciplines.
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