高危型人乳头瘤病毒患者宫颈脱落细胞中端粒的相对长度。

IF 3.5 4区医学 Q3 CELL BIOLOGY

Pathobiology Pub Date : 2024-01-01 Epub Date: 2023-11-01 DOI:10.1159/000534917

Rana Al-Awadhi, Materah Salem Alwehaidah, Moody AlRoomy, Kusum Kapila

{"title":"高危型人乳头瘤病毒患者宫颈脱落细胞中端粒的相对长度。","authors":"Rana Al-Awadhi, Materah Salem Alwehaidah, Moody AlRoomy, Kusum Kapila","doi":"10.1159/000534917","DOIUrl":null,"url":null,"abstract":"Introduction: This study investigates and compares the relative telomere length (RTL) outcome of high-risk (hr) human papillomavirus (HPV)-infected normal, low-grade squamous intraepithelial lesion (LSIL), and high-grade squamous intraepithelial lesion (HSIL) cervical samples to HPV-free normal cervical samples.Methods: This study used archived cervical samples and obtained cytology and histology data. HPV genotyping was conducted using Sanger sequencing, and RTL was performed using real-time quantitative polymerase chain reaction.Results: This study investigated 287 cervical samples, including 100 normal and hr-HPV-negative samples from the control group, 44 normal and hr-HPV-infected samples, and 143 SIL and hr-HPV-infected samples. The RTL in hr-HPV-infected samples, including the SIL and normal sample groups, was significantly longer than that in the control group. RTL in HSIL (5.13 ± 3.22) and LSIL (2.86 ± 2.81) was significantly different (p < 0.001). The RTL of cervical intraepithelial neoplasia (CIN1) lesion (3.53 ± 2.53) differed significantly (p < 0.001) when compared to CIN2 and CIN3 lesions combined (12.04 ± 10.51). The risk of developing cervical cancer was associated with RTL and decreased with RTL.Conclusion: This study revealed the strong potential of the RTL test in identifying women at risk of developing cervical cancer.","PeriodicalId":19805,"journal":{"name":"Pathobiology","volume":" ","pages":"180-186"},"PeriodicalIF":3.5000,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Relative Telomere Length in Cervical Exfoliated Cells among Women with High-Risk Human Papillomavirus.\",\"authors\":\"Rana Al-Awadhi, Materah Salem Alwehaidah, Moody AlRoomy, Kusum Kapila\",\"doi\":\"10.1159/000534917\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Introduction: This study investigates and compares the relative telomere length (RTL) outcome of high-risk (hr) human papillomavirus (HPV)-infected normal, low-grade squamous intraepithelial lesion (LSIL), and high-grade squamous intraepithelial lesion (HSIL) cervical samples to HPV-free normal cervical samples.Methods: This study used archived cervical samples and obtained cytology and histology data. HPV genotyping was conducted using Sanger sequencing, and RTL was performed using real-time quantitative polymerase chain reaction.Results: This study investigated 287 cervical samples, including 100 normal and hr-HPV-negative samples from the control group, 44 normal and hr-HPV-infected samples, and 143 SIL and hr-HPV-infected samples. The RTL in hr-HPV-infected samples, including the SIL and normal sample groups, was significantly longer than that in the control group. RTL in HSIL (5.13 ± 3.22) and LSIL (2.86 ± 2.81) was significantly different (p < 0.001). The RTL of cervical intraepithelial neoplasia (CIN1) lesion (3.53 ± 2.53) differed significantly (p < 0.001) when compared to CIN2 and CIN3 lesions combined (12.04 ± 10.51). The risk of developing cervical cancer was associated with RTL and decreased with RTL.Conclusion: This study revealed the strong potential of the RTL test in identifying women at risk of developing cervical cancer.\",\"PeriodicalId\":19805,\"journal\":{\"name\":\"Pathobiology\",\"volume\":\" \",\"pages\":\"180-186\"},\"PeriodicalIF\":3.5000,\"publicationDate\":\"2024-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Pathobiology\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1159/000534917\",\"RegionNum\":4,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2023/11/1 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pathobiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1159/000534917","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2023/11/1 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

引言：本研究调查并比较了高危（hr）人乳头瘤病毒（HPV）感染的正常、低度鳞状上皮内病变（LSIL）和高度鳞状上皮内损伤（HSIL）宫颈样本与无HPV的正常宫颈样本的相对端粒长度（RTL）结果。方法：本研究使用存档的宫颈样本，并获得细胞学和组织学数据。使用Sanger测序进行HPV基因分型，使用实时定量聚合酶链式反应进行RTL。结果：本研究调查了287份宫颈样本，包括对照组的100份正常和hr HPV阴性样本，44份正常和hr-HPV感染样本，以及143份SIL和hr-HHPV感染样本。hr HPV感染样本（包括SIL和正常样本组）的RTL明显长于对照组。HSIL（5.13±3.22）和LSIL（2.86±2.81）的RTL有显著性差异（P＜0.001）。宫颈上皮内瘤变（CIN1）病变的RTL（3.53±2.53）与CIN2和CIN3联合病变相比有显著性差别（P＜0.01）。结论：RTL检测在识别有宫颈癌症风险的女性中具有很强的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Relative Telomere Length in Cervical Exfoliated Cells among Women with High-Risk Human Papillomavirus.

Introduction: This study investigates and compares the relative telomere length (RTL) outcome of high-risk (hr) human papillomavirus (HPV)-infected normal, low-grade squamous intraepithelial lesion (LSIL), and high-grade squamous intraepithelial lesion (HSIL) cervical samples to HPV-free normal cervical samples.

Methods: This study used archived cervical samples and obtained cytology and histology data. HPV genotyping was conducted using Sanger sequencing, and RTL was performed using real-time quantitative polymerase chain reaction.

Results: This study investigated 287 cervical samples, including 100 normal and hr-HPV-negative samples from the control group, 44 normal and hr-HPV-infected samples, and 143 SIL and hr-HPV-infected samples. The RTL in hr-HPV-infected samples, including the SIL and normal sample groups, was significantly longer than that in the control group. RTL in HSIL (5.13 ± 3.22) and LSIL (2.86 ± 2.81) was significantly different (p < 0.001). The RTL of cervical intraepithelial neoplasia (CIN1) lesion (3.53 ± 2.53) differed significantly (p < 0.001) when compared to CIN2 and CIN3 lesions combined (12.04 ± 10.51). The risk of developing cervical cancer was associated with RTL and decreased with RTL.

Conclusion: This study revealed the strong potential of the RTL test in identifying women at risk of developing cervical cancer.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Pathobiology 医学-病理学

CiteScore

8.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： ''Pathobiology'' offers a valuable platform for the publication of high-quality original research into the mechanisms underlying human disease. Aiming to serve as a bridge between basic biomedical research and clinical medicine, the journal welcomes articles from scientific areas such as pathology, oncology, anatomy, virology, internal medicine, surgery, cell and molecular biology, and immunology. Published bimonthly, ''Pathobiology'' features original research papers and reviews on translational research. The journal offers the possibility to publish proceedings of meetings dedicated to one particular topic.