组织miRNA谱与人类肾移植中的急性肾小管坏死、排斥表型和BK多瘤病毒相关肾病相关。

IF 2.3 4区 医学 Q2 UROLOGY & NEPHROLOGY
Nephron Pub Date : 2024-01-01 Epub Date: 2023-10-31 DOI:10.1159/000534072
Neva Bezeljak, Nika Kojc, Miha Arnol, Željka Večerić-Haler, Emanuela Boštjančič
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引用次数: 0

摘要

引言:微小核糖核酸是一种短的非编码核糖核酸,主要通过抑制信使核糖核酸的翻译来参与基因表达的调节。在生理条件下,miRNA参与病毒感染和免疫反应等;miRNA的异常表达与肾移植病理有关,但后期的全面比较,特别是在组织切片中,仍有待进行。方法:我们使用miRNA的全基因组筛选来确定那些可能参与肾移植后疾病过程的miRNA。从福尔马林固定石蜡包埋的肾活检样品中分离RNA。研究包括8例急性肾小管坏死(ATN)患者、8例抗体介导的排斥反应(ABMR)患者、10例T细胞介导的排异反应(TCMR)患者,10例BK多瘤病毒相关肾病(BKPyVAN)患者和12例同种异体移植物功能稳定且无重大异常(正常移植物,CTRL)患者的监测活检。结果:我们发现136个miRNA在病变肾移植组织中与正常移植物相比有差异表达;其中,74个miRNAs在ABMR中差异表达,65个在ATN中,62个在BKPyVAN中,69个在TCMR中,16个miRNA与特定疾病表型无关。此外,29个miRNA在ABMR和ATN之间有不同的表达,39个在BKPyVAN和TCMR之间,20个在BKPyVAN和ABMR之间,38个在ABMR与TCMR之间。这一发现有可能帮助临床医生做出治疗决策,并转化为对患者的非侵入性监测,例如血液样本。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Tissue miRNA Profile Is Associated with Acute Tubular Necrosis, Rejection Phenotypes and BK Polyomavirus-Associated Nephropathy in Human Kidney Allografts.

Introduction: MicroRNAs (miRNAs), short noncoding RNAs, are involved in the modulation of gene expression, mainly by inhibiting the translation of mRNAs. Under physiological conditions, miRNAs are involved in viral infections and immune responses, among others; aberrant miRNA expression has been associated with kidney transplant pathologies, but a comprehensive comparison of later, particularly in tissue sections, is still pending.

Methods: We used the genome-wide screening of miRNAs to identify those potentially involved in the disease processes after kidney transplantation. RNA was isolated from formalin-fixed paraffin-embedded kidney biopsy samples. Study included 8 patients with acute tubular necrosis (ATN), 8 patients with antibody-mediated rejection (ABMR), 10 patients with T-cell-mediated rejection (TCMR), 10 patients with BK polyomavirus-associated nephropathy (BKPyVAN), and 12 surveillance biopsies from patients with stable allograft function and no major abnormalities (normal allografts, CTRL).

Results: We found 136 miRNAs differentially expressed in diseased kidney transplant tissue compared with normal allografts; of these, 74 miRNAs were differentially expressed in ABMR, 65 in ATN, 62 in BKPyVAN, 69 in TCMR, and 16 miRNAs were not associated with a specific disease phenotype. In addition, 29 miRNAs were differently expressed between ABMR and ATN, 39 between BKPyVAN and TCMR, and 20 between BKPyVAN and ABMR, and 38 between ABMR and TCMR.

Conclusion: Our findings show that miRNA derived from kidney allograft biopsy samples represent an additional diagnostic tool to distinguish different disease phenotypes. This finding has the potential to assist clinicians in therapeutic decision-making and to translate to noninvasive monitoring of patients, e.g., blood samples.

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来源期刊
Nephron
Nephron UROLOGY & NEPHROLOGY-
CiteScore
5.00
自引率
0.00%
发文量
80
期刊介绍: ''Nephron'' comprises three sections, which are each under the editorship of internationally recognized leaders and served by specialized Associate Editors. Apart from high-quality original research, ''Nephron'' publishes invited reviews/minireviews on up-to-date topics. Papers undergo an innovative and transparent peer review process encompassing a Presentation Report which assesses and summarizes the presentation of the paper in an unbiased and standardized way.
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