5xFAD小鼠海马星形胶质细胞表达增加

Violeta Jovanović, Jelica Despotović, Mario Balo, I. Zaletel, Sanja Z Despotović, N. Puškaš
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引用次数: 0

摘要

简介:阿尔茨海默病是最常见的神经退行性疾病,其特征是淀粉样斑块和神经原纤维缠结在患者的大脑中形成,导致神经元损伤和丧失。星形胶质细胞的激活和星形胶质形成与此过程同时发生。由于在人体组织中工作的伦理限制,已经开发了许多转基因动物模型来研究这些过程的发病机制。在皮层和海马中观察到早期Ab沉积。目的:本研究旨在确定36周龄转基因5xFAD小鼠海马中GFAP阳性细胞的存在与相应对照的差异。材料和方法:采用5xFAD老年痴呆症小鼠模型,其特点是淀粉样蛋白斑块早期形成,但不存在神经纤维缠结。转基因动物和对照动物在36周龄时被处死。采用免疫组化和胶质原纤维酸性蛋白(GFAP)抗体(星形胶质细胞的主要标记物)对脑海马区GFAP阳性细胞进行可视化。免疫反应性定量采用Icy软件系统。结果:36周龄时,转基因鼠与对照组海马齿状回和海马颗粒区GFAP表达差异有统计学意义,CA1-3区差异无统计学意义。结论:所获得的结果证实了星形胶质细胞形成参与了阿尔茨海默病的病理生理,并且表明在36周龄的5xFAD小鼠中,相对于海马的其他区域,星形胶质细胞形成在齿状回和颗粒区发生得更早。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Increased astrocyte representation in the hippocampus of 5xFAD mice
Introduction: Alzheimer's disease is the most common neurodegenerative disorder, characterized by the formation of amyloid plaques and the neurofibrillary tangles in the brain of an ill person, leading to neuronal damage and loss. Activation of astrocytes and astrogliosis occurs along with this process. Due to ethical limitations in working with human tissue, numerous transgenic animal models have been developed to study the pathogenesis of these processes. Early Ab deposition is observed in the cortex and the hippocampus. Aim: This study aimed to determine the difference in the presence of GFAP positive cells in the hippocampus between transgenic 5xFAD mice aged 36 weeks and their corresponding controls. Material and Methods: The 5xFAD mice model of Alzheimer's disease was used, characterized by early formation of amyloid plaques but without the presence of neurofibrillar tangles. Transgenic and control animals were sacrificed at 36 weeks of age. The visualization of GFAP-positive cells in the hippocampus of their brains was done by using immunohistochemistry and antibody for glial fibrillary acidic protein - GFAP, the major marker of astrocytes. Quantification of immuno-reactivity was done by using the Icy software system. Results: There was a statistically significant difference in the expression of GFAP in the dentate gyrus and the granular zone of the hippocampus between the transgenic and control group at 36 weeks of age, while the significant change in the CA1-3 regions was not observed between investigated groups. Conclusion: Obtained results confirm the involvement of astrogliosis in the pathophysiology of Alzheimer's disease and indicate an earlier occurrence of astrogliosis in the dentate gyrus and granular zone, in relation to other regions of the hippocampus, in the 36-week-old 5xFAD mice.
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