天然生物群对碳酸钙的生物沉淀作为一种修复方法。

IF 0.2 Q4 ENGINEERING, MULTIDISCIPLINARY
L. Aguilera, J. A. N. Zapata, Benjamín Otto Ortega Morales
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引用次数: 7

摘要

选取了30种以前分离到的不同类型的细菌,主要是芽孢杆菌,这些细菌以前通过微生物学和分子方法鉴定为来自墨西哥市查普尔特佩克城堡的外生生物膜。采用不同的方法确定了其中哪些形成了生物膜,并对其进行了定量、尿素测试和碳酸钙结晶的生产,并在Petry板上富集了0.2%的碳酸钙。所得晶体经x射线衍射和扫描电镜分析。结果表明,所有被测微生物都参与了生物膜的形成过程,只有12株分离菌株,包括IS16 (Bacillus subtilis);蜡样芽孢杆菌(Bacillus cereus)和枯草芽孢杆菌(Bacillus subtilis)菌株IIS15a和IIIS10是最佳的生物成膜菌,UFC / mL高,对碳酸盐具有良好的生物沉淀效果。其中,菌株IIIS4(巨型芽孢杆菌)、IS5(枯草芽孢杆菌)、IIIS9b(枯草芽孢杆菌)、21 (Pantoea agglomerans)和IIIS5(单纯芽孢杆菌)。可能有些微生物能够产生脲酶,诱导方解石的沉淀,有助于恢复。到目前为止,关于Pantoea团聚体通过碳酸钙晶体的生物沉淀参与生物修复过程的了解很少,由于尿素测试获得了良好的反应,并且获得的晶体主要是Calcita(99%),这项工作表明了利用这种天然微生物进行活化以实现磨损古迹修复的可行性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
La bioprecipitación de carbonato de calcio por la biota nativa como un método de restauración.
A selection of 30 previously isolated bacteria of different types were taken, mainly Bacillus, which had been previously identified by microbiological and molecular methods to be epilithic biofilms from Chapultepec's Castle in Mexico City, DF. Different methodologies were practiced to determine which of them were forming biofilms and their quantification, the ureasa test and the production of crystals of calcium carbonate in Petry plate with Luria Bertani enriched with calcium carbonate to 0.2 %. The crystals obtained were submitted to diffraction of X-rays and Electronic Microscopy of Sweep. The results show that all microorganisms tested were involved in the process of biofilm formation, and only 12 isolates, including the IS16 (Bacillus subtilis); IIS15a (Bacillus cereus) and IIIS10 (Bacillus subtilis) strains, were the best biofilm formers with high UFC / mL, good in terms of bioprecipitation of carbonates. Particularly, the strains IIIS4 (Bacillus megaterium), IS5 (Bacillus subtilis), IIIS9b (Bacillus subtilis), 21 (Pantoea agglomerans) and IIIS5 (Bacillus simplex). May be some of the microorganisms could be capable of producing urease, to induce precipitation of calcite that contributes to the restoration. As of now, little is known about the participation of Pantoea agglomerans in processes of biorrestauration by means of bioprecipitation of crystals of calcium carbonate and due to the fact that a good response was obtained from the test of ureasa and the crystals obtained were mainly Calcita (99 %), this work shows the feasibility of using this native microorganisms for their activation to achieve the restoration of the worn monuments.
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来源期刊
Nexo Revista Cientifica
Nexo Revista Cientifica ENGINEERING, MULTIDISCIPLINARY-
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审稿时长
30 weeks
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