ZWINT通过调节p53/p21信号通路促进宫颈癌症细胞的增殖、迁移和侵袭。

IF 16.4 1区 化学 Q1 CHEMISTRY, MULTIDISCIPLINARY
Zhe Ma, Yufei Cai, Chenchen Tian
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引用次数: 0

摘要

癌症每年导致30万人死亡,宫颈癌发生机制尚不清楚。Zeste-White 10相互作用的动粒蛋白(ZWINT)在恶性肿瘤中独特地升高,促进癌症细胞的增殖、迁移和集落形成。探讨ZWINT在宫颈癌症增殖、迁移、侵袭中的作用,评价其作为治疗靶点的潜在能力。首先,使用生物信息学方法分析了ZWINT在癌症宫颈癌中的表达,并在几个癌症宫颈癌细胞系中进行了评估。细胞活力和集落形成测定用于评估细胞增殖。然后,进行transwell分析以研究细胞迁移和侵袭。此外,用蛋白质印迹法测定ZWINT、基质金属蛋白酶9(MMP-9)、N-钙粘蛋白、E-钙粘蛋白,p53和p21在ZWINT过表达或敲低的CaSki和HeLa细胞中的表达水平。生物信息学分析和蛋白质印迹分析显示,ZWINT在宫颈癌症中的表达显著增加。细胞活力和集落形成分析表明,ZWINT过表达可促进细胞增殖,而ZWINT敲低可抑制细胞增殖。此外,ZWINT通过调节MMP-9、N-钙粘蛋白和E-钙粘蛋白的表达,促进CaSki和HeLa细胞的迁移和侵袭。此外,ZWINT减弱了宫颈癌症细胞中p53和p21的表达。总之,ZWINT通过抑制p53/p21信号通路,促进宫颈癌症细胞的增殖、迁移和侵袭,这表明ZWINT是宫颈癌症治疗的潜在治疗靶点。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ZWINT promotes the proliferation, migration, and invasion of cervical cancer cells by regulating the p53/p21 signaling pathway.

Cervical cancer leads to 300,000 deaths annually and the mechanism of cervical carcinogenesis remains unclear. Zeste White 10-interacting kinetochore protein (ZWINT) is uniquely elevated in malignancies, promoting proliferation, migration, and colony formation of cancer cells. To investigate the role of ZWINT in proliferation, migration, invasion of cervical cancer, and evaluate the potential ability of ZWINT as a therapeutic target. First, ZWINT expression in cervical cancer was analyzed using the bioinformatic methods and assessed in several cervical cancer cell lines. The cell viability and colony formation assays were used to evaluate cell proliferation. Then, transwell assay was performed to investigate cell migration and invasion. Moreover, western blot was used to measure the expression level of ZWINT, matrix metalloproteinase 9 (MMP-9), N-cadherin, E-cadherin, p53 and p21 in CaSki and HeLa cells with ZWINT overexpression or knockdown. The bioinformatic analysis and western blot assay revealed the expression of ZWINT was significantly increased in cervical cancer. The cell viability and colony formation analysis illustrated that cell proliferation could be promoted by ZWINT overexpression and suppressed by ZWINT knockdown. Moreover, ZWINT promoted migration and invasion of CaSki and HeLa cells, through regulating the expression of MMP-9, N-cadherin, and E-cadherin. Furthermore, ZWINT attenuated the expression of p53 and p21 in cervical cancer cells. In summary, ZWINT functions in promoting cell proliferation, migration, and invasion of cervical cancer cells by suppressing p53/p21 signaling pathway, which indicated ZWINT is a potential therapeutic target for cervical cancer treatment.

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来源期刊
Accounts of Chemical Research
Accounts of Chemical Research 化学-化学综合
CiteScore
31.40
自引率
1.10%
发文量
312
审稿时长
2 months
期刊介绍: Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.
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