利用肽印迹膜减少淀粉样蛋白沉积的新策略

C. Cristallini, E. Bellotti, F. Spezia, E. Rosellini, M. Cascone, N. Barbani
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引用次数: 3

摘要

大脑中β淀粉样蛋白的积累导致认知障碍,在阿尔茨海默病等神经退行性病理中观察到。目前的研究旨在验证一种假设,即通过体外净化系统从血液中快速去除β淀粉样蛋白肽,可能是治疗这种神经退行性疾病患者的另一种解决方案。在这方面,我们研究了基于聚乙烯醇的分子印迹膜对淀粉样蛋白片段25-35 (AbP)的特异性识别特性,AbP是淀粉样蛋白中更具有神经毒性的区域。用丁二酸酐对共聚物主链进行化学改性,有利于形成羧基,从而提高印迹性能。物理化学、形态学、力学和功能表征给出了有趣的结果,证实了印迹膜在体外再结合AbP的能力。结论这项工作代表了一种生物相容性聚合物膜的概念证明,这种聚合物膜能够选择性地从血液和脑脊液中去除淀粉样蛋白肽。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
A New Strategy to Reduce Amyloid Deposition using Peptide-Imprinted Membranes
Background The accumulation of amyloid beta protein in the brain causes the cognitive impairment observed in neurodegenerative pathologies such as Alzheimer's disease. The present study aimed to test the hypothesis that a rapid removal of amyloid beta protein peptides from the blood by an extracorporeal purification system could represent an alternative solution for the treatment of patients suffering from this neurodegenerative disease. Methods In this regard, we investigated the specific recognition properties of a molecularly imprinted membrane based on poly(ethylene-co-vinyl alcohol) toward the amyloid beta protein fragment 25-35 (AbP), the more neurotoxic domain of amyloid beta protein. A chemical modification of the copolymer backbone using succinic anhydride was also performed to favor the formation of carboxylic groups and thus improve imprinting performance. Results The physico-chemical, morphological, mechanical and functional characterisations gave interesting results confirming the ability of imprinted membranes to in vitro rebind AbP. Conclusions This work represents a proof of concept regarding the development of a biocompatible polymer membrane capable of selectively removing amyloid beta peptide from the blood and consequently from the cerebrospinal fluid.
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来源期刊
Journal of Applied Biomaterials & Biomechanics
Journal of Applied Biomaterials & Biomechanics 生物-材料科学:生物材料
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