非功能化石墨烯纳米片薄膜上皮层初级神经元细胞的生长和随访

Shiyun Meng, Rong Peng
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引用次数: 7

摘要

导电性生物材料是通过传导内部或外部电信号来改变细胞行为的理想生物基质。本研究基于石墨在有机试剂中剥离的简单制备方法,通过喷涂简单制备了高导电性、大尺寸的非功能化石墨烯纳米片薄膜(NGNF)。用原代皮层神经元细胞仔细测试了NGNF的生物相容性,并将其与化学气相沉积(CVD)石墨烯膜的生物相容性进行了比较。方法采用平头超声探头从石墨表面剥离非官能化石墨烯纳米片(NGN),然后将其喷涂在玻片基底上形成薄膜。光镜和扫描电镜观察了NGNF的形态。DAPI染色和Alexa Fluor®488染色观察NGNF上皮层神经元细胞的形态和神经元网络的形成。MTT法检测细胞活力和增殖。结果NGNF具有比CVD石墨烯膜更好的细胞生物相容性。MTT试验显示NGNF无细胞毒性。细胞培养第7天神经元网络形成情况显示,原代神经元细胞聚集成50 μm的“核”;平均神经突数为3 μm,神经突长度为100 μm。然而,细胞培养14天后,这些值几乎翻了一番。结论NGNF可作为神经再生的导电支架。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Growth and Follow-Up of Primary Cortical Neuron Cells on Nonfunctionalized Graphene Nanosheet Film
Background Conductive biomaterials are an ideal biosubstrate for modifying cellular behaviors by conducting either internal or external electrical signals. In this study, based on a simple-preparation graphite exfoliation method in organic reagent, a nonfunctionalized graphene nanosheet film (NGNF) with high conductivity and large size was simply fabricated through spraying coating. The biocompatibility of the NGNF was carefully tested with primary cortical neuron cells, and its biocompatibility properties were compared with a chemical vapor deposition (CVD) graphene film. Methods Nonfunctionalized graphene nanosheet (NGN) was first exfoliated from graphite with a flat-tip ultrasonicator probe, and then spray-coated onto glass slide substrate to form the film. The morphology of NGNF was observed with light microscopy and SEM. The morphology and neuronal network formation of primary cortical neuron cells onto NGNF, as shown by DAPI and Alexa Fluor® 488 staining, were observed with fluorescent microscopy. Cell viability and proliferation were measured with MTT. Results NGNF had better cell biocompatibility than CVD graphene film. MTT test showed that NGNF exhibited no cytotoxicity. According to neuronal network formation at 7 days of cell culture, primary neuron cells aggregated into 50-μm “nuclei”; the average neurite number and length were 3 and 100 μm, respectively. However, these values were almost doubled after 14 days of cell culture. Conclusions These results may improve the use of NGNF as a conductive scaffold for nerve regeneration.
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来源期刊
Journal of Applied Biomaterials & Biomechanics
Journal of Applied Biomaterials & Biomechanics 生物-材料科学:生物材料
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