大豆、玉米及其饮料中总酚含量及体外抗氧化潜力的研究

PAUL CHIDOKA CHIKEZIE
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引用次数: 1

摘要

本研究试图通过体外抗氧化评价模型,将未加工大豆(SB)及其工业加工饮料(SBB1和SBB2)和未加工玉米(SM)及其工业加工饮料(MBB1和MBB2)的总酚含量(TPC)与抗氧化潜力联系起来。采用标准分光光度法测定样品的TPC和抗氧化电位。自由基清除能力指数(SCI50)定义了清除50%自由基所需的样品浓度,单位为μg/mL。同样,AP50定义了还原50%铁离子所需的样品浓度,单位为μg/mL。SB、SBB1和SBB2的TPC在0.97±0.02 ~ 2.86±0.02 mg没食子酸当量/ g干样范围内,SM、MBB1和MBB2的TPC依次递增:SM>MBB1>MBB2。SB、SBB1和SBB2对NO -、H2O2和•- OH的TPC及其对应的SCI50的相关系数在-0.77227 ~ 0.338172单位之间,而它们对应的AP50呈强正相关。SM、MBB1和MBB2对NO -、H2O2和•- OH的TPC及其对应的SCI50的相关系数在0.040672 ~ 0.51799单位之间,而它们对应的AP50呈较强的负相关。研究表明,抗氧化潜力与各种样品的组合抗氧化特性交织在一起。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Total Phenolic Contents and Antioxidant Potential of Soya Bean and Maize and their Beverages In vitro
The present investigations sought to correlate the total phenolic contents (TPC) with antioxidant potential, using in vitro antioxidant evaluation models, of unprocessed soya bean (SB) and its industrial processed beverages (SBB1 and SBB2) as well as unprocessed maize (SM) and its industrial processed beverages (MBB1 and MBB2). The TPC and antioxidant potential of the samples were measured using standard spectrophotometric methods. The radical scavenging capacity index (SCI50) defined the concentration, in μg/mL, of the sample required to scavenge 50% of the investigated radicals. Likewise, AP50 defined the concentration, in μg/mL, of the sample required to reduce 50% of ferric ion. The TPC of SB, SBB1 and SBB2 was within the range of 0.97 ± 0.02-2.86 ± 0.02 mg gallic acid equivalent per gram dry sample, and TPC of SM, MBB1 and MBB2 were in the increasing order: SM>MBB1>MBB2. The TPC of SB, SBB1 and SBB2 and their corresponding SCI50 against NO–, H2O2 and •−OH gave correlation coefficients between the range: -0.77227-0.338172 units, whereas their corresponding AP50 gave a strong positive correlation. The TPC of SM, MBB1 and MBB2 and their corresponding SCI50 against NO–, H2O2 and •−OH gave correlation coefficients between the range: 0.040672-0.51799 units, whereas their corresponding AP50 showed a strong negative correlation. The study revealed that antioxidant potential was intertwined with the combinatorial antioxidant peculiarities of the various samples.
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