六味地黄丸治疗甲亢的血清代谢组学研究

Q4 Pharmacology, Toxicology and Pharmaceutics

中国药理学通报 Pub Date : 2013-05-01 DOI:10.3969/J.ISSN.1001-1978.2013.05.010

Bin Wang, Lan Shen, W. Cong, Yunyun Zhu, Qiang Wang, Yi Feng

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引用次数: 0

摘要

目的:通过血清代谢组学方法，探讨皮下注射左旋甲状腺素钠致大鼠甲状腺功能亢进的机制及六味地黄丸对大鼠甲状腺功能亢进的干预作用。方法:给大鼠注射左旋甲状腺素钠，剂量为35 μg·(100 g)-1，每天1周，然后给六味地黄丸，剂量为4.7 g·kg-1，连续3周。采用超高效液相色谱-飞行时间质谱法(UPLC-QTOF-MS)测定大鼠LDPS处理后的血清代谢谱。结果:与正常大鼠相比，模型组大鼠体内草酰乙酸、磷酸二羟丙酮(DHAP)、葡萄糖醛酸乙酯、甘油三酯、溶血磷脂、胆固醇水平均显著升高。而且，在LDPS治疗后，这些水平可以被调节到接近正常水平。结论:甲亢大鼠三羧酸循环及糖脂代谢存在严重紊乱。LDPS对甲亢的治疗效果明显。血清代谢组学分析揭示了LDPS治疗过程中代谢物性状的变化，从而为LDPS治疗甲亢提供了一种补充评价方法。

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Serum metabonomic study on liuwei dihuang pills in the treatment of hyperthyroidism

Aim: To explore the underlying mechanism of hyperthyroidism induced by subcutaneous injection of L-levothyroxine sodium and and intervention effects of Liuwei Dihuang pills on sprague dawley rats via serum metabolomics. Methods: After rats were injected with L-levothyroxine sodium at a dosage of 35 μg·(100 g)-1 every day for 1 week,and then Liuwei dihuang pills(LDPs) were treated at a dose of 4.7 g·kg-1 for 3 consecutive weeks.The serum samples for rats were obtained after the treatment of LDPS and the metabolic profiles of serum were obtained with ultra performance liquid chromatography coupled with time-of-flight mass spectrometry(UPLC-QTOF-MS). Results: The levels of oxaloacetic acid,Dihydroxyacetone phosphate(DHAP),ethyl glucuronide,triglyceride,lysophospholipids and cholesterol were significantly increased in the model group as compared to the normal rats.Moreover,these levels could be regulated to be close to normal after the treatment of LDPS. Conclusions: There is severe disturbance in the tricarboxylic acid cycle as well as sugar and lipid metabolism for hyperthyroidism rats.LDPS can exhibite obvious effects on the treatment of hyperthyroidism.Serum metabonomics analysis reveals the changes of metabolites traits during the treatment of LDPS,thus providing a supplementary evaluation method of LDPS in the treatment of hyperthyroidism.

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来源期刊

中国药理学通报 Pharmacology, Toxicology and Pharmaceutics-Pharmacology

CiteScore

0.80

自引率

0.00%

发文量

11201

期刊介绍： Chinese Pharmacological Bulletin (CPB) is an academic monthly magazine sponsored by the Chinese Association for Science and Technology, the Chinese Pharmacological Society, and Anhui Medical University. Publicly issued domestically and internationally, CN:34:1086/R,ISSN:1001-1978。 The inclusion status of Chinese Pharmaceutical Bulletin (CPB): 1. This journal has been identified as a core medical journal by the China Science Citation Database (CSCD) of the Document Information Center of the Chinese Academy of Sciences (since 1993, there are 909 journals in the core library); 2. Selected as a pharmaceutical core journal by the editor in chief of Peking University Library's "Overview of Chinese Core Journals" (Peking University Core), 1st to 8th editions (1243 scientific and technological journals, including 232 medical and health journals); 3. Designated by the Institute of Science and Technology Information of the Ministry of Science and Technology of China as a source journal for Chinese scientific and technological paper statistics (China Science and Technology Core Journal); 4. Other: Domestic: Chinese Academic Journal Abstracts, Chinese Basic Medical Abstracts, Chinese Pharmaceutical Abstracts, Chinese Biological Abstracts, Chinese Academic Journals (CD version), Wanfang Database, VIP Database, etc; International: Chemical Abstract (USA), Corrective Bulletin on Science and Technology, CBST (Japan), Biological Abstract (USA), Biochemical Abstract (USA), EMBASE/Excerpta Medica (Netherlands), Index Copernicus (Poland), Kunst and Wissen (Germany), WPRIM (World Health Organization Western Pacific Regional Medical Index), Scopus (Netherlands, 2019 CiteScore 0.9), A&I (Netherlands), Centre for Agriculture and Biosciences international (CAB international, UK), Biosis Previews (USA), etc.