伊朗人群中肿瘤坏死因子- α基因rs1800629 (-308G/A)和rs361525 (-238G /A)多态性与前列腺癌风险的关系

Q3 Medicine
Saeideh Alidoost, Mohsen Habibi, Z. Noormohammadi, J. Hosseini, E. Azargashb, F. Pouresmaeili
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引用次数: 3

摘要

前列腺癌(PCa)是世界上第一大男性常见癌症,也是伊朗男性的第三大癌症,它是一种异质性疾病,有时需要多次活检才能诊断。目的寻找新的生物标志物,以便尽早诊断前列腺癌。因此,我们研究了TNF-α基因rs1800629和rs361525多态性与PCa的关系。材料与方法采集100例PCa患者、110例BPH患者和110例对照组的血液DNA样本。收集的样品使用PCR-RFLP和Tetra-ARMS-PCR技术检测所需的多态性。结果吸烟者与非吸烟者的rs1800629基因型频率差异有统计学意义(p= 0.001)。Logistic回归分析结果显示,与GG纯合子相比,GA杂合子发生良性前列腺增生(BPH)或前列腺癌的风险更高。然而,未考虑PCa风险与TNF-α基因多态性(rs1800629和rs361525)之间的显著相关性。尽管如此,本研究取得的结果表明,在本初步研究中,这两种检测的遗传变异似乎不适合作为前列腺癌早期诊断的标记;然而,GA杂合子和吸烟者患前列腺癌的风险增加,这表明一些表观遗传因素影响前列腺癌的病因。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Association between tumor necrosis factor-alpha gene rs1800629 (-308G/A) and rs361525 (-238G > A) polymorphisms and prostate cancer risk in an Iranian cohort.
BACKGROUND Prostate cancer (PCa) as the first men's common cancer in the world and the third cancer in Iranian men is a heterogeneous disorder which sometimes several biopsies are needed for its diagnosis. OBJECTIVES The aim of current study is finding new biomarkers in order to diagnose of PCa at the earliest possible stage. Hence, the relationship between rs1800629 and rs361525 polymorphisms of TNF-α gene with PCa was investigated. MATERIALS AND METHODS Blood DNA samples were collected from 100 patients with PCa, 110 with BPH, and 110 controls. Collected samples were examined using PCR-RFLP and Tetra-ARMS-PCR techniques to detect the desired polymorphisms. RESULTS The frequency of rs1800629 genotypes in smokers was significantly different from non-smokers with PCa (p= 0.001). Logistic regression analysis results showed that GA heterozygotes in comparison to GG homozygotes had higher risk of developing Benign Prostatic Hyperplasia (BPH) or prostate cancer. However, no significant correlation was considered between the risk of PCa and the TNF-α gene polymorphisms (rs1800629 and rs361525). CONCLUSIONS Although, the achieved results of this investigation demonstrated that the two examined genetic variants do not seem to be suitable markers for early diagnosis of prostate cancer in this pilot study; however increased risk for the disease is shown in GA heterozygotes and smokers which is indicative of some epigenetic factors influence on prostate cancer etiology.
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来源期刊
Human Antibodies
Human Antibodies Medicine-Immunology and Allergy
CiteScore
3.50
自引率
0.00%
发文量
27
期刊介绍: Human Antibodies is an international journal designed to bring together all aspects of human hybridomas and antibody technology under a single, cohesive theme. This includes fundamental research, applied science and clinical applications. Emphasis in the published articles is on antisera, monoclonal antibodies, fusion partners, EBV transformation, transfections, in vitro immunization, defined antigens, tissue reactivity, scale-up production, chimeric antibodies, autoimmunity, natural antibodies/immune response, anti-idiotypes, and hybridomas secreting interesting growth factors. Immunoregulatory molecules, including T cell hybridomas, will also be featured.
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