长链非编码RNA HIF1A-AS1对大鼠缺血心肌再灌注损伤的调控作用

Q4 Medicine

海军军医大学学报 Pub Date : 2015-01-01 DOI:10.3724/SP.J.1008.2015.00131

Guan‐xin Zhang, B. Cong, Jiajun Zhang, Chong Wang, Yang Yuan, Guo-Kun Wang, Lin Han, Xu Zhiyun

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引用次数: 1

摘要

目的研究长链非编码RNA HIF1A-AS1对心肌缺血再灌注(I/R)损伤的调控作用及其机制。方法用SD大鼠建立心肌I/R损伤模型，用大鼠心肌细胞建立缺氧再氧化(H/R)模型。使用si-HIF1A-AS1抑制HIF1A-AS1在心肌中的表达。实时荧光定量PCR检测HIF1A-AS1 mRNA表达，MTT法检测心肌细胞生长活力，ELISA法检测培养基中乳酸脱氢酶(LDH)浓度，Western blotting法检测自噬相关蛋白beclin -1表达。结果HIF1A-AS1在I/R模型大鼠心肌和H/R模型大鼠心肌细胞中表达升高。siRNA抑制HIF1A-AS1可保护心肌细胞免受H/R损伤，逆转H/R刺激诱导的心肌生长活力下降、培养基中LDH水平升高、自噬相关蛋白beclin -1表达增加。结论抑制长链非编码RNA HIF1A-AS1可能通过抑制心肌细胞过度自噬对心肌I/R损伤起保护作用。

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Regulatory effects of long non-coding RNA HIF1A-AS1 on ischemic myocardial reperfusion injury in rats

Objective To study the regulatory effects of long non-coding RNA HIF1A-AS1 on the myocardial ischemia reperfusion(I/R)injury and the related mechanism.Methods Myocardial I/R injury model was established with SD rats,and hypoxia reoxygenation(H/R)model was established with rat cardiac myocytes.si-HIF1A-AS1 was used to inhibit HIF1A-AS1 expression in the cardiac myoctyes.Then the mRNA expression of HIF1A-AS1 was detected by real-time PCR,the growth vitality of cardiac myocytes was investigated by MTT assay,the concentration of lactate dehydrogenase(LDH)in the culture media was detected by ELISA,and the autophagy-associated protein Beclin-1expression was observed by Western blotting analysis.Results HIF1A-AS1 expression was increased in cardiac muscle of rat I/R model and rat cardiac myocytes of H/R model.Inhibition of HIF1A-AS1 by siRNA protected the cardiomyocytes against H/R injuries,reversing the decreased growth vitality of cardiac myoctyes,increased LDH level in the culture media,and increased expression of autophagy-related protein Beclin-1induced by H/R stimulation.Conclusion Inhibition of long non-coding RNA HIF1A-AS1 might play aprotective role in I/R injury of cardiac myoctyes by inhibiting the excessive autophagy of cardiomyocytes.

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来源期刊

海军军医大学学报 Medicine-Medicine (all)

CiteScore

0.50

自引率

0.00%

发文量

14752

期刊介绍： Founded in 1980, Academic Journal of Second Military Medical University(AJSMMU) is sponsored by Second Military Medical University, a well-known medical university in China. AJSMMU is a peer-reviewed biomedical journal,published in Chinese with English abstracts.The journal aims to showcase outstanding research articles from all areas of biology and medicine,including basic medicine(such as biochemistry, microbiology, molecular biology, genetics, etc.),clinical medicine,public health and epidemiology, military medicine,pharmacology and Traditional Chinese Medicine),to publish significant case report, and to provide both perspectives on personal experiences in medicine and reviews of the current state of biology and medicine.