骨关节炎滑液与蛋白浓度的关系。

IF 1 4区医学 Q4 BIOPHYSICS

Biorheology Pub Date : 2016-01-01 DOI:10.3233/BIR-15086

A. Madkhali, Michael Chernos, S. Fakhraei, D. Grecov, E. Kwok

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引用次数: 4

摘要

背景:骨关节炎是一种常见的局部关节疾病，可引起疼痛、僵硬和活动能力降低。骨关节炎对膝关节滑液伸展流变的影响尚不完全清楚，因此需要进一步研究。目的研究骨关节炎滑膜液的外延流变性，探讨滑膜液蛋白浓度与外延流变性之间的关系。该研究还将探讨与剪切流变学的可能相关性。方法采用毛细管破裂拉伸流变仪对21例骨关节炎滑膜液进行拉伸流变研究。在先前的研究中，拉伸流变性能与蛋白质浓度和剪切流变性能相关。在拉伸流动条件下进行了粘补充试验，并进行了比较。结果拉伸流变性能发现试样之间的差异很大，但在一般情况下发现与以往的研究一致。拉伸流变特性与蛋白质浓度之间没有统计学上的显著相关性。零剪切粘度与末端拉伸粘度(R-squared = 0.73)、零剪切粘度与拉伸松弛时间(R-squared = 0.84)、剪切松弛时间与拉伸松弛时间(R-squared = 0.75)呈正相关。结论确定了适合骨关节炎滑膜液研究的CaBER操作参数。蛋白浓度与拉伸流变参数之间无统计学意义的相关性。几个剪切和拉伸流变参数之间存在正相关关系。最近对死后滑液的研究发现，滑液的拉伸粘度和松弛时间的报告值在一个数量级内。

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Osteoarthritic synovial fluid and correlations with protein concentration.

BACKGROUND Osteoarthritis is a common, localized joint disease that causes pain, stiffness and reduced mobility. The effects of osteoarthritis on the extensional rheology of synovial fluid in the knees are not fully understood and consequently require further study. OBJECTIVE The purpose of this work is to study the extensional rheology of osteoarthritic synovial fluid and to investigate a possible correlation between synovial fluid protein concentration and extensional rheology. The study will also investigate possible correlations with the shear rheology. METHODS 21 osteoarthritic synovial fluid samples were studied under extensional flow with a capillary breakup extensional rheometer. Extensional rheological properties were correlated with protein concentration and with shear rheological properties measured in a prior study. Viscosupplement was also studied under extensional flow for comparison. RESULTS Extensional rheological properties were found to vary widely between samples, but in general were found to agree with previous studies. No statistically significant correlation was identified between extensional rheological properties and protein concentration. Positive correlations were identified between zero shear viscosity and terminal extensional viscosity (R-squared = 0.73), zero shear viscosity and extensional relaxation time (R-squared = 0.84), and shear relaxation time and extensional relaxation time (R-squared = 0.75). CONCLUSIONS Appropriate CaBER operating parameters for study of osteoarthritic synovial fluid were identified. No statistically significant correlation was found to exist between protein concentration and extensional rheological parameters. Positive correlations were identified between several shear and extensional rheological parameters. The reported values for extensional viscosity and relaxation times for synovial fluid were found to be within one order of magnitude with a recent study of post mortem synovial fluid.

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来源期刊

Biorheology 医学-工程：生物医学

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Biorheology is an international interdisciplinary journal that publishes research on the deformation and flow properties of biological systems or materials. It is the aim of the editors and publishers of Biorheology to bring together contributions from those working in various fields of biorheological research from all over the world. A diverse editorial board with broad international representation provides guidance and expertise in wide-ranging applications of rheological methods to biological systems and materials. The scope of papers solicited by Biorheology extends to systems at different levels of organization that have never been studied before, or, if studied previously, have either never been analyzed in terms of their rheological properties or have not been studied from the point of view of the rheological matching between their structural and functional properties. This biorheological approach applies in particular to molecular studies where changes of physical properties and conformation are investigated without reference to how the process actually takes place, how the forces generated are matched to the properties of the structures and environment concerned, proper time scales, or what structures or strength of structures are required.