半角蝎毒液对大鼠心脏的毒性作用

Q4 Pharmacology, Toxicology and Pharmaceutics

Iranian Journal of Toxicology Pub Date : 2021-01-01 DOI:10.32598/IJT.15.1.691.1

Moein Yazdkhasti, M. Jalali, G. Khadjeh, H. Jafari, A. Rezaie

{"title":"半角蝎毒液对大鼠心脏的毒性作用","authors":"Moein Yazdkhasti, M. Jalali, G. Khadjeh, H. Jafari, A. Rezaie","doi":"10.32598/IJT.15.1.691.1","DOIUrl":null,"url":null,"abstract":"Methods: The scorpion’s venom was extracted into six fractions by chromotagraphy. Healthy male Wistar rats (N=72) were equally divided into eight groups of nine: G1: Controls (0.5ml. normal saline), G2: Crude venom (1000μg/kg), G3: F-I (120μg/kg), G4: F-II (430μg/kg), G5: F-III (80 μg/kg), G6: F-IV (180μg/kg), G7: F-V (60μg/kg), and G8: F-VI (130μg/kg). Blood samples were obtained by cardiac puncture at 1, 3 and 24 hours after the venom injection. The serum levels of AST, LDH, CPK, CK-MB and troponin-I were determined. Upon euthanasia, the hearts were removed from the rats and examined microscopically for histopathology.","PeriodicalId":14637,"journal":{"name":"Iranian Journal of Toxicology","volume":"15 1","pages":"27-36"},"PeriodicalIF":0.0000,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":"{\"title\":\"Cardiotoxic Effects of Hemiscorpius Lepturus Scorpion Venom Fractions in Rats\",\"authors\":\"Moein Yazdkhasti, M. Jalali, G. Khadjeh, H. Jafari, A. Rezaie\",\"doi\":\"10.32598/IJT.15.1.691.1\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Methods: The scorpion’s venom was extracted into six fractions by chromotagraphy. Healthy male Wistar rats (N=72) were equally divided into eight groups of nine: G1: Controls (0.5ml. normal saline), G2: Crude venom (1000μg/kg), G3: F-I (120μg/kg), G4: F-II (430μg/kg), G5: F-III (80 μg/kg), G6: F-IV (180μg/kg), G7: F-V (60μg/kg), and G8: F-VI (130μg/kg). Blood samples were obtained by cardiac puncture at 1, 3 and 24 hours after the venom injection. The serum levels of AST, LDH, CPK, CK-MB and troponin-I were determined. Upon euthanasia, the hearts were removed from the rats and examined microscopically for histopathology.\",\"PeriodicalId\":14637,\"journal\":{\"name\":\"Iranian Journal of Toxicology\",\"volume\":\"15 1\",\"pages\":\"27-36\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2021-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"3\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Iranian Journal of Toxicology\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.32598/IJT.15.1.691.1\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"Pharmacology, Toxicology and Pharmaceutics\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Toxicology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.32598/IJT.15.1.691.1","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Pharmacology, Toxicology and Pharmaceutics","Score":null,"Total":0}

引用次数: 3

摘要

方法:采用色谱法提取蝎子毒液，提取成6个部分。健康雄性Wistar大鼠72只，平均分为8组，每组9只:G1组:对照组(0.5ml;G2:粗毒液(1000μg/kg)、G3: F-I (120μg/kg)、G4: F-II (430μg/kg)、G5: F-III (80 μg/kg)、G6: F-IV (180μg/kg)、G7: F-V (60μg/kg)、G8: F-VI (130μg/kg)。注射毒液后1、3、24小时穿刺取血。测定血清AST、LDH、CPK、CK-MB、肌钙蛋白i水平。安乐死后，从大鼠身上取出心脏，用显微镜检查组织病理学。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

查看原文本刊更多论文

Cardiotoxic Effects of Hemiscorpius Lepturus Scorpion Venom Fractions in Rats

Methods: The scorpion’s venom was extracted into six fractions by chromotagraphy. Healthy male Wistar rats (N=72) were equally divided into eight groups of nine: G1: Controls (0.5ml. normal saline), G2: Crude venom (1000μg/kg), G3: F-I (120μg/kg), G4: F-II (430μg/kg), G5: F-III (80 μg/kg), G6: F-IV (180μg/kg), G7: F-V (60μg/kg), and G8: F-VI (130μg/kg). Blood samples were obtained by cardiac puncture at 1, 3 and 24 hours after the venom injection. The serum levels of AST, LDH, CPK, CK-MB and troponin-I were determined. Upon euthanasia, the hearts were removed from the rats and examined microscopically for histopathology.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Iranian Journal of Toxicology Environmental Science-Health, Toxicology and Mutagenesis

CiteScore

1.60

自引率

0.00%

发文量

审稿时长

9 weeks