母羊在性周期和怀孕期间阴道涂片的细胞学特征

S. Vlasenko, O. Zhulinska, O. Yeroshenko
{"title":"母羊在性周期和怀孕期间阴道涂片的细胞学特征","authors":"S. Vlasenko, O. Zhulinska, O. Yeroshenko","doi":"10.33245/2310-4902-2022-173-1-6-13","DOIUrl":null,"url":null,"abstract":"New criteria for cytological analysis of sheep vaginal swabs (cytovaginal examination) during sexual hunting, during the sexual cycle, after insemination and depending on the effectiveness of insemination have been developed. Cytological material was collected from the area of the upper vaginal vault using a sterile vaginal mirror, forceps and cotton gauze swab. Staining of brushstrokes was performed according to Gimza-Romanovsky. To improve the method of cytovaginal examination, the division of smear epitheliocytes into groups: functional, destroyed, non-nuclear and cells with nuclear pyknosis is proposed. In turn, among the functional cells it is advisable to distinguish between cells of the deep layers of the epithelium (basal-parabasal), intermediate and surface layer (superficial nuclear). The main types of ratio of particles of different groups and subgroups and their prognostic value in the mating season during sexual hunting are revealed. It was found that during the inhibition stage there is an intensive desquamation of the epithelial level, which was manifested by a 2.5-fold decrease in the number of basal / parabasal epitheliocytes and almost four - destroyed epitheliocytes. And already from the 13th day there is an increase in the cells of the functional level due to the proportion of cells of the deep and intermediate level. On the 18th day, the cellular composition of vaginal smears in sheep acquired characteristics characteristic of the stage of arousal. Thus, compared with the 6-8th day of the cycle, the number of destroyed epitheliocytes (p <0.001) and naked nuclei (up to 8.3 ± 1.6) doubled again (up to 12.0 ± 0.7) against the background of a decrease in 4 times (to the initial level) of cells with pyknosis of the nucleus. The main cytological sign of pregnancy from the fourth day after insemination was a 2.7-fold increase in the number of destroyed cells and \"naked\" nuclei. In the following days of the study, except for the 13th day, this difference ranged from 1.3 to 4.3 times (8.3 ± 5.4 to 14.7 ± 3.6%), compared with the corresponding figures in unfertilized ewes. Also on the 13th day after the introduction of sperm in fertilized females there is a tendency to increase the number of deep layer cells and the acquisition on the 18th day of a probable difference in the ratio between the number of deep and surface cells - 5: 1. Changes in the distribution of deep and surface cell particles in unfertilized animals indicate the manifestation of the next stage of excitation and the predominance of epithelial cell differentiation. In contrast, in pregnant females, the proliferation of the deep layers of the vaginal epithelium increased.\nKey words: ewes, sexual cycle, sexual hunting, vaginal smear, cytological composition, pregnancy.","PeriodicalId":34230,"journal":{"name":"Naukovii visnik veterinarnoyi meditsini","volume":"1 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Cytological characteristics of vaginal smears in ewes during the sexual cycle and during pregnancy\",\"authors\":\"S. Vlasenko, O. Zhulinska, O. Yeroshenko\",\"doi\":\"10.33245/2310-4902-2022-173-1-6-13\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"New criteria for cytological analysis of sheep vaginal swabs (cytovaginal examination) during sexual hunting, during the sexual cycle, after insemination and depending on the effectiveness of insemination have been developed. Cytological material was collected from the area of the upper vaginal vault using a sterile vaginal mirror, forceps and cotton gauze swab. Staining of brushstrokes was performed according to Gimza-Romanovsky. To improve the method of cytovaginal examination, the division of smear epitheliocytes into groups: functional, destroyed, non-nuclear and cells with nuclear pyknosis is proposed. In turn, among the functional cells it is advisable to distinguish between cells of the deep layers of the epithelium (basal-parabasal), intermediate and surface layer (superficial nuclear). The main types of ratio of particles of different groups and subgroups and their prognostic value in the mating season during sexual hunting are revealed. It was found that during the inhibition stage there is an intensive desquamation of the epithelial level, which was manifested by a 2.5-fold decrease in the number of basal / parabasal epitheliocytes and almost four - destroyed epitheliocytes. And already from the 13th day there is an increase in the cells of the functional level due to the proportion of cells of the deep and intermediate level. On the 18th day, the cellular composition of vaginal smears in sheep acquired characteristics characteristic of the stage of arousal. Thus, compared with the 6-8th day of the cycle, the number of destroyed epitheliocytes (p <0.001) and naked nuclei (up to 8.3 ± 1.6) doubled again (up to 12.0 ± 0.7) against the background of a decrease in 4 times (to the initial level) of cells with pyknosis of the nucleus. The main cytological sign of pregnancy from the fourth day after insemination was a 2.7-fold increase in the number of destroyed cells and \\\"naked\\\" nuclei. In the following days of the study, except for the 13th day, this difference ranged from 1.3 to 4.3 times (8.3 ± 5.4 to 14.7 ± 3.6%), compared with the corresponding figures in unfertilized ewes. Also on the 13th day after the introduction of sperm in fertilized females there is a tendency to increase the number of deep layer cells and the acquisition on the 18th day of a probable difference in the ratio between the number of deep and surface cells - 5: 1. Changes in the distribution of deep and surface cell particles in unfertilized animals indicate the manifestation of the next stage of excitation and the predominance of epithelial cell differentiation. In contrast, in pregnant females, the proliferation of the deep layers of the vaginal epithelium increased.\\nKey words: ewes, sexual cycle, sexual hunting, vaginal smear, cytological composition, pregnancy.\",\"PeriodicalId\":34230,\"journal\":{\"name\":\"Naukovii visnik veterinarnoyi meditsini\",\"volume\":\"1 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-06-24\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Naukovii visnik veterinarnoyi meditsini\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.33245/2310-4902-2022-173-1-6-13\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"\",\"JCRName\":\"\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Naukovii visnik veterinarnoyi meditsini","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.33245/2310-4902-2022-173-1-6-13","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

摘要

在性狩猎期间,在性周期期间,在授精后以及根据授精效果对绵羊阴道拭子进行细胞学分析(阴道细胞检查)的新标准已经制定。使用无菌阴道镜、镊子和棉纱棒从阴道上穹窿区域收集细胞学材料。根据Gimza-Romanovsky进行笔触染色。为了改进阴道细胞检查方法,提出将涂片上皮细胞分为功能细胞、破坏细胞、无核细胞和核固缩细胞。反过来,在功能细胞中,最好区分上皮深层(基底-旁基底)、中间层和表层(浅核)的细胞。揭示了不同类群和亚类群颗粒比例的主要类型及其在交配季节的预测价值。结果发现,在抑制阶段,上皮水平发生强烈的脱屑,表现为基底/副基底上皮细胞数量减少2.5倍,上皮细胞几乎被破坏4个。从第13天开始,由于深层和中级细胞的比例,功能水平的细胞增加了。在第18天,绵羊阴道涂片的细胞组成获得了觉醒阶段的特征。因此,与周期的6-8天相比,破坏的上皮细胞(p <0.001)和裸核(高达8.3±1.6)的数量再次增加一倍(高达12.0±0.7),而核固缩细胞的数量减少了4倍(达到初始水平)。从授精后第4天开始,怀孕的主要细胞学迹象是破坏细胞和“裸”核数量增加2.7倍。在接下来的研究中,除第13天外,与未受精母羊相比,这一差异为1.3 ~ 4.3倍(8.3±5.4 ~ 14.7±3.6%)。在受精后的第13天,精子进入雌性体内后,深层细胞的数量有增加的趋势,在第18天,深层细胞和表层细胞的数量之间的比例可能会出现5:1的差异。未受精动物深部和表面细胞颗粒分布的变化表明下一阶段兴奋的表现和上皮细胞分化的优势。相反,在怀孕的女性中,阴道上皮的深层增殖增加。关键词:母羊,性周期,性狩猎,阴道涂片,细胞学成分,妊娠。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytological characteristics of vaginal smears in ewes during the sexual cycle and during pregnancy
New criteria for cytological analysis of sheep vaginal swabs (cytovaginal examination) during sexual hunting, during the sexual cycle, after insemination and depending on the effectiveness of insemination have been developed. Cytological material was collected from the area of the upper vaginal vault using a sterile vaginal mirror, forceps and cotton gauze swab. Staining of brushstrokes was performed according to Gimza-Romanovsky. To improve the method of cytovaginal examination, the division of smear epitheliocytes into groups: functional, destroyed, non-nuclear and cells with nuclear pyknosis is proposed. In turn, among the functional cells it is advisable to distinguish between cells of the deep layers of the epithelium (basal-parabasal), intermediate and surface layer (superficial nuclear). The main types of ratio of particles of different groups and subgroups and their prognostic value in the mating season during sexual hunting are revealed. It was found that during the inhibition stage there is an intensive desquamation of the epithelial level, which was manifested by a 2.5-fold decrease in the number of basal / parabasal epitheliocytes and almost four - destroyed epitheliocytes. And already from the 13th day there is an increase in the cells of the functional level due to the proportion of cells of the deep and intermediate level. On the 18th day, the cellular composition of vaginal smears in sheep acquired characteristics characteristic of the stage of arousal. Thus, compared with the 6-8th day of the cycle, the number of destroyed epitheliocytes (p <0.001) and naked nuclei (up to 8.3 ± 1.6) doubled again (up to 12.0 ± 0.7) against the background of a decrease in 4 times (to the initial level) of cells with pyknosis of the nucleus. The main cytological sign of pregnancy from the fourth day after insemination was a 2.7-fold increase in the number of destroyed cells and "naked" nuclei. In the following days of the study, except for the 13th day, this difference ranged from 1.3 to 4.3 times (8.3 ± 5.4 to 14.7 ± 3.6%), compared with the corresponding figures in unfertilized ewes. Also on the 13th day after the introduction of sperm in fertilized females there is a tendency to increase the number of deep layer cells and the acquisition on the 18th day of a probable difference in the ratio between the number of deep and surface cells - 5: 1. Changes in the distribution of deep and surface cell particles in unfertilized animals indicate the manifestation of the next stage of excitation and the predominance of epithelial cell differentiation. In contrast, in pregnant females, the proliferation of the deep layers of the vaginal epithelium increased. Key words: ewes, sexual cycle, sexual hunting, vaginal smear, cytological composition, pregnancy.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
自引率
0.00%
发文量
0
审稿时长
10 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信