细胞因子在健康动物和患有化脓性坏死肢突的奶牛中的分布

V. Melnikov, M. Rublenko, M. Ilnitskyi
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引用次数: 0

摘要

机体对任何组织和器官损伤的创伤或感染因子的典型和强制性反应之一是急性期反应,这是一种诱导的合成增加,随后在血液和组织中增加一些具有免疫、杀菌、抗氧化和抑制功能的蛋白质。本研究的目的是确定临床健康奶牛、猪和狗以及四肢有脓坏死过程的奶牛体内细胞因子的水平。测定牛、猪和狗血清中促炎细胞因子和抗炎IL-10的水平。将四肢化脓性坏死病变奶牛(n=26)分为:第一组(n=8):远端急性化脓性坏死病变奶牛;第二组(n=8) -全身性病变;第三组(n=10) -手指区域复发灶。按标准方案采用免疫酶法测定血清中TNF-α、IL-1ß、IL-10白介素的含量。结果表明,临床健康奶牛血液中促炎因子TNF-α和IL-1 β水平显著低于抗炎因子IL-10, IL-10与TNF-α - 3.3:1、IL-10与IL-1 β - 9.5:1的比值表明,在生理正常条件下,牛具有固有的抗炎因子谱。在临床健康猪中,抗炎细胞因子谱最明显,细胞因子指数显著升高:IL-10:TNF-α - 19.4:1;Il-10: il-1β - 13.9:1。在临床健康犬血液中,IL-10:IL-1ß指数极低- 1.5:1,TNF-α:IL-1ß - 0.2:1甚至更低,IL-10:TNF-α - 8.8:1之间,因此从细胞因子指数的总体来看,抗炎细胞因子谱要低得多。急性坏死性细菌病奶牛的血液中TNF-α水平比临床健康动物高5.6倍(Р<0.001), IL-1ß水平比临床健康动物高3.4倍(Р<0.001),其增加指数为1.7倍,达到4.9:1。在此条件下,IL-10水平仅升高1.8倍(p <0.05)。与急性型相比,全身型动物的血液中TNF-α和il -1 β水平分别临界增加16.8倍和17.8倍(p <0.001),而IL-10水平保持不变。细胞因子指数IL-10:TNF-α达到临界值- 0.4:1,IL-10达到IL-1ß - 1:1。在复发性病变的动物中,低水平的IL-1ß,特别是IL-10是特征。但TNF-α浓度仍然很高,超过正常值的12.6倍(p<0.001)。因此,各种临床形式的奶牛肢体坏死性细菌病变具有代偿性或非代偿性细胞分裂血症,由于病牛抑制潜能能力不足,急性期蛋白功能失衡。关键词:牛、猪、狗,细胞因子,血清,临床健康动物,炎症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cytokine profiles in healthy animals and in cows with purulent-necrotic limb processes
One of the typical and mandatory reactions of the body to trauma or infectious agents for damage to any tissues and organs is the acute phase reaction, which is an induced increase in the synthesis followed by an increase in the blood and tissues of a number of proteins with immunological, bactericidal, antioxidant and inhibitory functions. The purpose of the study is to determine the levels of cytokines in clinically healthy cows, pigs and dogs, as well as in cows with purulent-necrotic processes of the limbs. The levels of pro-inflammatory cytokines and anti-inflammatory IL-10 were determined in blood serum of cows, pigs and dogs. Cows with purulent-necrotic lesions of the limbs (n=26) were divided into: 1st group (n=8) – acute form of purulent-necrotic lesions of the distal limbs; 2nd group (n=8) – generalized lesions; group 3 (n=10) – recurrent foci in the area of the fingers. The content of TNF-α, IL-1ß and IL-10 interleukins in blood serum was determined by the immunoenzymatic method according to the standard protocol. It was established that the blood levels of pro-inflammatory TNF-α and IL-1ß in clinically healthy cows are significantly lower than anti-inflammatory IL-10, for the ratio of IL-10:TNF-α – 3.3:1, and IL- 10: IL- 1ß - 9.5:1, therefore, for cattle under physiological norms, an inherent anti-inflammatory cytokine profile. In clinically healthy pigs, the anti-inflammatory cytokine profile turned out to be the most pronounced, as the cytokine indices in them were significantly higher: IL-10:TNF-α – 19.4:1; IL-10:IL-1β – 13.9:1. In the blood of clinically healthy dogs, the cytokine indices between IL-10:IL-1ß are extremely low - 1.5:1, TNF-α : IL-1ß - 0.2:1, even lower, and between IL-10:TNF-α - 8.8:1, and therefore the anti-inflammatory cytokine profile is much lower according to the totality of cytokine indices. In cows with an acute form of necrobacteriosis, compared with clinically healthy animals, the level of TNF-α in the blood is 5.6 times higher (Р<0.001), and IL-1ß is 3.4 times higher (Р<0.001), due to their increase index by 1.7 times, up to 4.9:1. Under such conditions, the level of IL-10 increases only 1.8 times (р<0.05). Animals with the generalized form are characterized by a critical increase in the blood level of TNF-α by 16.8 times and IL-1ß by 17.8 times (p <0.001), while the level of IL-10 remains unchanged compared to the acute form. Cytokine index IL-10:TNF-α acquires a critical value - 0.4:1, and IL-10 to IL-1ß - 1:1. In animals with recurrent lesions, low levels of IL-1ß, especially IL-10, are characteristic. However, the concentration of TNF-α remains quite high and exceeds the normal value by 12.6 times (p<0.001). Therefore, various clinical forms of necrobacterial lesions of the limbs in cows have a compensatory or non-compensatory nature of cytokinemia, with an imbalance of the functionality of acute-phase proteins due to the insufficient capacity of the inhibitory potential of sick cows. Key words: cows, pigs, dogs, cytokines, serum, clinically healthy animals, inflammation.
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