猪链球菌野外分离株微生物学特征及耐药性研究

M. Savcheniuk, L. Kornienko, O. Tarasov, O. Dovgal, S. Bilyk, V. Dovhenko, T. Tsarenko
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摘要

猪链球菌是引起猪脑膜炎、败血症、肺炎、心内膜炎和关节炎等多种疾病的重要相关病原体。猪流感可通过直接接触患病动物传染给人类。感染链球菌的猪不仅会造成严重的直接损害,而且还会导致病毒感染的传播,如PRRS,在受链球菌影响的猪场中,80%的病例都是PRRS。近年来,链球菌感染的流行率显著增加,它们作为病毒性和细菌性疾病的一个复杂因素的作用也显著增加。不同群体抗生素的不合理使用是该病传播的一个重要因素,导致致病菌群迅速获得多耐药。本文报道了从病猪病理标本中分离的猪链球菌的微生物学特性和耐药性的研究结果。为了更好、更快地分离出病原菌,研究了其对仔猪脏器组织的损伤特点及其在仔猪体内的定位。对乌克兰养猪场的链球菌疫情进行了动物流行病监测。进行采样的猪场对仔猪链球菌永远不利。对猪链球菌现场分离物的实验室研究发现,所有被研究的微生物都能发酵精氨酸、水杨苷、糖原、d -葡萄糖、蔗糖、半乳糖、麦芽糖、水杨苷、海藻糖、菊糖,并对α-半乳糖苷酶、β-葡糖苷和β-葡糖苷有阳性反应。其中,87%的猪链球菌对红霉素敏感,75%对克林霉素敏感,55%对头孢氨苄敏感,43%对庆大霉素耐药,20%对恩诺沙星耐药。结果发现,在25%的病例中,猪葡萄球菌以急性单感染的形式引起疾病,在20%的病例中,猪葡萄球菌分离出大肠杆菌,在16%的病例中(多杀性巴氏杆菌),在11%的病例中(副猪嗜血杆菌)。混合感染占17%。关键词:猪链球菌;抗生素耐药性;培养特性;
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microbiological characteristics and antibiotic resistance of field isolates Streptococcus suis
Streptococcus suis is an important associated pathogen with a wide range of diseases in pigs such as meningitis, septicemia, pneumonia, endocarditis and arthritis. S suis can be transmitted to humans through direct contact with a sick animal. The disease of pigs with streptococcus not only causes significant direct damage, but also contributes to the spread of viral infections such as PRRS, which is registered in 80% of cases on farms affected by streptococcus. In recent years, there has been a significant increase in the prevalence of streptococcal infections, as well as their role as a complicating factor in viral and bacterial diseases. An important factor in the spread of this disease is the irrational use of antibiotics of different groups, which contributes to the rapid acquisition of polyresistance of pathogenic microflora. The article presents the results of studies on the microbiological characteristics and antibiotic resistance of isolates of Streptococcus suis isolated from pathological material from sick pigs. In order to better and faster isolation of the pathogen, the features of its damage to organs and tissues and its localization in the body of piglets were studied. Epizootological monitoring of streptococcal outbreaks on pig farms in Ukraine was carried out. The farms on the basis of which the sampling was carried out are permanently unfavorable for streptococcus of piglets. Laboratory studies of S. suis field isolates found that all studied microorganisms fermented arginine, salicin, glycogen, D-glucose, sucrose, galactose, maltose, salicin, trehalose, inulin, with positive reactions to α-galactosidase, β-glucutane and β-glucura . Of the isolated Str. suis isolates, 87% were sensitive to erythromycin, 75% to clindamycin and 55% to cephalexin, 43% of isolates were resistant to gentamicin and 20% to enrofloxacin. It was found that in 25% of cases S. suis caused the disease in the form of acute monoinfection, in 20% of cases S. suis isolated E. coli, in 16% of cases - with Pasteurella multocida, in 11% of cases - with Haemophilus parasuis. Mixed infection was observed in 17% of cases. Key words: Streptococcus suis, antibiotic resistance, culture properties, diagnostic methods.
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