流动相ph对高效液相色谱分离药物的影响

IF 2.4 4区医学 Q3 CHEMISTRY, MEDICINAL

Drug Development and Industrial Pharmacy Pub Date : 1995-01-01 DOI:10.3109/03639049509026647

V. Gupta, M. Mathew

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引用次数: 7

摘要

摘要在建立药物的高效液相色谱分析方法时，流动相的pH值可以有效地作为一种工具来完成药物的完全分离。采用两种弱酸(氢化可的松琥珀酸钠和甲基强的松琥珀酸钠)和一种弱碱(盐酸异丙嗪)，在其他条件不变的情况下，测定了流动相在7种不同pH值下色谱图(保留时间和峰分离)的变化。结果表明，弱酸的保留时间随pH值的增加而减少，弱碱的保留时间随pH值的增加而增加，确定了这两种药物的最佳分离pH值为5。

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Effect of Mobile Phase ph on the Separation of Drugs using High-Performance Liquid Chromatography

AbstractWhen developing a HPLC assay method for drugs, the pH of the mobile phase can be effectively used as a tool to accomplish complete separation. Using two weak acids (hydrocortisone sodium succinate and methylprednisolone sodium succinate) and a weak base (promethazine hydrochloride), the changes in the chromatograms (retention times and separation of peaks) have been determined at 7 different pH values of a mobile phase with other conditions remaining the same. It has been shown that the retention times of weak acids will decrease with increasing pH values while those of weak bases will increase with increasing pH. The optimum pH value for the separation of these drugs has been determined to be 5.

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来源期刊

Drug Development and Industrial Pharmacy 医学-药学

CiteScore

6.80

自引率

0.00%

发文量

审稿时长

4.5 months

期刊介绍： The aim of Drug Development and Industrial Pharmacy is to publish novel, original, peer-reviewed research manuscripts within relevant topics and research methods related to pharmaceutical research and development, and industrial pharmacy. Research papers must be hypothesis driven and emphasize innovative breakthrough topics in pharmaceutics and drug delivery. The journal will also consider timely critical review papers.