hplc - nne13 CNMR耦合指纹图谱分析技术及其在丁香抗肝纤维化活性研究中的应用

Zhi-xiang Zhang, W. Yin, Pu Liu, T. Zhao
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引用次数: 1

摘要

本研究描述了紫丁香的有效成分,已被确定为能够防止肝纤维化。本文报道了用于耦合指纹分析的高效液相色谱和非核overhauser效应碳-13核磁共振(hplc - nne13 CNMR)技术的特点。该方法的主要贡献是为利用色谱和光谱耦合指纹图谱技术分析中药提供了一种有效的技术和有用的工具。二环烯醚萜苷类化合物的分离及构效关系的研究表明,这些衍生物是丁香抗肝纤维化的有效成分,是丁香抗肝纤维化的重要成分。通过hplc - nne13 CNMR耦合指纹图谱分析,鉴定其活性成分为橄榄苦苷、10-羟基橄榄苦苷、橄榄苷-11-甲基lester、(8Z)-灯叶苷和紫锥花苷。HPLC- nne13cnmr耦合指纹图谱法也证实了浓度-响应关系。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
HPLC-NNE 13 CNMR coupling fingerprint analysis technology and its application in a study of Syringa pubescens Turcz and its activity against hepatic fibrosis
This study describes the active ingredients of Syringa pubescens Turcz which has been identified as being able to protect against hepatic fibrosis. Here we report the characteris - tics of high performance liquid chromatography and non-nuclear overhauser effect carbon-13 nuclear magnetic resonance (HPLC-NNE 13 CNMR) technology developed for coupling finger - print analysis. The major contribution of this new method is the development of an efficient technology and a useful tool for analysis of a traditional Chinese herbal medicine using chro- matography and spectral coupling fingerprint technology. Isolation of secoiridoid glycosides and investigation of their structure-activity relationship showed that these derivatives are the active ingredients of Syringa pubescens Turcz, and account for the activity of this plant against hepatic fibrosis. The active compounds were identified as oleuropein, 10-hydroxyoleuropein, oleoside-11-methylester, (8Z)-ligstroside, and echinacoside by HPLC-NNE 13 CNMR coupling fingerprint analysis. A concentration-response relationship was also demonstrated for the HPLC- NNE 13 CNMR coupling fingerprint method.
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