废水相关水体中鱼类不动杆菌种类的MALDI-TOF质谱分析

Q3 Veterinary
Popovic Topic, S. Kazazić, R. Čož-Rakovac
{"title":"废水相关水体中鱼类不动杆菌种类的MALDI-TOF质谱分析","authors":"Popovic Topic, S. Kazazić, R. Čož-Rakovac","doi":"10.2298/vetgl211201005t","DOIUrl":null,"url":null,"abstract":"Acinetobacter species are common inhabitants of freshwater and marine ecosystems with a capacity to induce disease in affected fish. To facilitate their rapid and reliable identification, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), was applied to Acinetobacter from fish. The aims of the study were, thus, to identify and profile the Acinetobacter species from tissues of fish living in a vulnerable environment impacted by wastewaters, and to assess the potential of MALDI-TOF MS as a method for discriminating these acinetobacters. Fish were sampled from waterways impacted by the activity of a wastewater treatment plant. Samples of gills, spleen, kidney and liver were streaked onto general-purpose media to purity. The profiling and identification of acinetobacters was conducted with MALDI-TOF MS, with the samples prepared by ethanol/formic acid extraction.The identified acinetobacters were retrieved from gills (68.96 %), kidney (13.79 %), liver (10.34 %), and spleen (6.89 %). The Acinetobacter species isolated from all tested fish tissues were A. johnsonii (79.31 %), A. pittii (10.34 %), A. tandoii (3.44 %), A. guilouiae (3.44 %), and A. gerneri (3.44 %). Highly probable and probable species identifications were obtained for 48.27 % of all acinetobacters tested, indicating fully reliable identification. MALDI?TOF MS gave excellent identification and profiling results for piscine Acinetobacter species from the wastewater-affected waterways. It is a recommendable technique for future Acinetobacter species discrimination, as accurate and rapid identification of these bacteria is critical in both environmental pollution management and human/veterinary clinical diagnostics.","PeriodicalId":30435,"journal":{"name":"Veterinarski Glasnik","volume":"59 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"MALDI-TOF MS profiling of piscine Acinetobacter species from wastewater-related waters\",\"authors\":\"Popovic Topic, S. Kazazić, R. Čož-Rakovac\",\"doi\":\"10.2298/vetgl211201005t\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Acinetobacter species are common inhabitants of freshwater and marine ecosystems with a capacity to induce disease in affected fish. To facilitate their rapid and reliable identification, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), was applied to Acinetobacter from fish. The aims of the study were, thus, to identify and profile the Acinetobacter species from tissues of fish living in a vulnerable environment impacted by wastewaters, and to assess the potential of MALDI-TOF MS as a method for discriminating these acinetobacters. Fish were sampled from waterways impacted by the activity of a wastewater treatment plant. Samples of gills, spleen, kidney and liver were streaked onto general-purpose media to purity. The profiling and identification of acinetobacters was conducted with MALDI-TOF MS, with the samples prepared by ethanol/formic acid extraction.The identified acinetobacters were retrieved from gills (68.96 %), kidney (13.79 %), liver (10.34 %), and spleen (6.89 %). The Acinetobacter species isolated from all tested fish tissues were A. johnsonii (79.31 %), A. pittii (10.34 %), A. tandoii (3.44 %), A. guilouiae (3.44 %), and A. gerneri (3.44 %). Highly probable and probable species identifications were obtained for 48.27 % of all acinetobacters tested, indicating fully reliable identification. MALDI?TOF MS gave excellent identification and profiling results for piscine Acinetobacter species from the wastewater-affected waterways. It is a recommendable technique for future Acinetobacter species discrimination, as accurate and rapid identification of these bacteria is critical in both environmental pollution management and human/veterinary clinical diagnostics.\",\"PeriodicalId\":30435,\"journal\":{\"name\":\"Veterinarski Glasnik\",\"volume\":\"59 1\",\"pages\":\"\"},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2022-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Veterinarski Glasnik\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.2298/vetgl211201005t\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Veterinary\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Veterinarski Glasnik","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2298/vetgl211201005t","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Veterinary","Score":null,"Total":0}
引用次数: 0

摘要

不动杆菌是淡水和海洋生态系统中常见的居民,有能力在受影响的鱼类中诱发疾病。为了快速、可靠地鉴定不动杆菌,采用基质辅助激光解吸/电离飞行时间质谱法(MALDI-TOF MS)对鱼类不动杆菌进行了鉴定。因此,本研究的目的是鉴定和分析生活在受废水影响的脆弱环境中的鱼类组织中的不动杆菌种类,并评估MALDI-TOF质谱作为鉴别这些不动杆菌的方法的潜力。从受污水处理厂活动影响的水道中取样鱼类。鳃、脾、肾和肝的样品在通用培养基上进行条纹染色以达到纯度。采用MALDI-TOF质谱法对不动杆菌进行分析和鉴定,样品采用乙醇/甲酸萃取法制备。检出的不动杆菌分别来自鳃(68.96%)、肾脏(13.79%)、肝脏(10.34%)和脾脏(6.89%)。从所有鱼组织中分离出的不动杆菌种类分别为:约氏不动杆菌(79.31%)、皮氏不动杆菌(10.34%)、坦多不动杆菌(3.44%)、吉乌伊不动杆菌(3.44%)和格纳伊不动杆菌(3.44%)。48.27%的不动杆菌得到了高度可能和可能的菌种鉴定,表明鉴定完全可靠。谱技术?TOF质谱对受污水影响的水体中的不动杆菌种类进行了极好的鉴定和分析。这是未来不动杆菌种类鉴别的一种推荐技术,因为这些细菌的准确和快速鉴定对环境污染管理和人类/兽医临床诊断至关重要。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
MALDI-TOF MS profiling of piscine Acinetobacter species from wastewater-related waters
Acinetobacter species are common inhabitants of freshwater and marine ecosystems with a capacity to induce disease in affected fish. To facilitate their rapid and reliable identification, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), was applied to Acinetobacter from fish. The aims of the study were, thus, to identify and profile the Acinetobacter species from tissues of fish living in a vulnerable environment impacted by wastewaters, and to assess the potential of MALDI-TOF MS as a method for discriminating these acinetobacters. Fish were sampled from waterways impacted by the activity of a wastewater treatment plant. Samples of gills, spleen, kidney and liver were streaked onto general-purpose media to purity. The profiling and identification of acinetobacters was conducted with MALDI-TOF MS, with the samples prepared by ethanol/formic acid extraction.The identified acinetobacters were retrieved from gills (68.96 %), kidney (13.79 %), liver (10.34 %), and spleen (6.89 %). The Acinetobacter species isolated from all tested fish tissues were A. johnsonii (79.31 %), A. pittii (10.34 %), A. tandoii (3.44 %), A. guilouiae (3.44 %), and A. gerneri (3.44 %). Highly probable and probable species identifications were obtained for 48.27 % of all acinetobacters tested, indicating fully reliable identification. MALDI?TOF MS gave excellent identification and profiling results for piscine Acinetobacter species from the wastewater-affected waterways. It is a recommendable technique for future Acinetobacter species discrimination, as accurate and rapid identification of these bacteria is critical in both environmental pollution management and human/veterinary clinical diagnostics.
求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Veterinarski Glasnik
Veterinarski Glasnik Veterinary-General Veterinary
CiteScore
1.10
自引率
0.00%
发文量
11
审稿时长
16 weeks
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信