白杨素- plga - peg纳米颗粒对胃癌细胞增殖及mirna基因表达的影响

F. Mohammadian, A. Abhari, H. Dariushnejad, A. Nikanfar, Younes Pilehvar-Soltanahmadi, N. Zarghami
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引用次数: 66

摘要

背景近年来,黄菊花素作为一种黄酮类化合物被发现具有肿瘤化学预防作用。本实验利用PLGA-PEG-chrysin复合物和游离chrysin来评价miR-22、miR-34a和miR-126在人胃细胞系中的表达。目的研究纳米包封金菊素是否能提高游离金菊素对AGS人胃癌细胞的抗癌作用。方法采用扫描电镜(SEM)、核磁共振(H NMR)、红外光谱(FTIR)等方法对聚乙二醇-聚乙二醇纳米颗粒包裹的杨菊素进行表征。采用MTT法对人胃细胞株生长进行了细胞毒性评价。用预先安排好的纯包膜的菊花素处理细胞后,提取RNA,用实时荧光定量PCR检测miR-22、miR-34a和miR-126的表达。结果在PLGA-PEG纳米颗粒的负载量下,纳米胶囊的IC50值与游离的相比显著降低。这一发现已经通过纳米胶囊化的白菊花素的miR-22, miR-34a和miR-126基因表达的进一步增加与游离白菊花素相比得到证实。结论在本研究中,我们发现PLGA-PEG-chrysin对人胃细胞系生长的抑制作用优于游离的chrysin。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Effects of Chrysin-PLGA-PEG Nanoparticles on Proliferation and Gene Expression of miRNAs in Gastric Cancer Cell Line
Background Recently, Chrysin, as a flavone, has revealed cancer chemo-preventive activity. The present experiment utilized the PLGA-PEG-chrysin complex, and free chrysin, to evaluation of the expression of miR-22, miR-34a and miR-126 in human gastric cell line. Objectives The purpose of this study was to examine whether nano encapsulating chrysin improves the anti-cancer effect of free chrysin on AGS human gastric cell line. Methods Properties of the chrysin encapsulated in PLGA-PEG nanoparticles were investigated by SEM, H NMR, and FTIR. The assessment of cytotoxicity on the growth of the human gastric cell line was carried out through MTT assay. After treating the cells with a prearranged amount of pure and encapsulated chrysin, RNA was extracted and the expressions of miR-22, miR-34a and miR-126 were measured by using real-time PCR. Results With regard to the amount of the chrysin loaded in PLGA-PEG nanoparticles, IC50 value was significantly decreased in nanocapsulatedchrysin, in comparison with free chrysin. This finding has been proved through the further increase of miR-22, miR-34a and miR-126 gene expression of nanocapsulatedchrysin, in comparison with free chrysin. Conclusions In this study, we revealed that the PLGA-PEG-chrysin is more effective than free chrysin in inhibiting the growth of human gastric cell line.
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