辅助因子依赖性酶中缺失辅助因子的两种测量方法的比较:比例与相对增加

Q3 Medicine

Open Biomarkers Journal Pub Date : 2012-11-30 DOI:10.2174/1875318301205010016

Tomas Hansson

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引用次数: 2

摘要

辅助因子依赖酶中缺少辅助因子的程度被广泛用作辅助因子缺乏的生物标志物。辅因子缺失的程度可以表示为不含辅因子的酶的比例，也可以表示为在样品中加入过量辅因子后含辅因子的酶的相对增加。特别是对于以焦磷酸酯矿(TPP)为辅助因子的酶，相对增加(TPP效应)已在大多数研究中使用，其使用似乎普遍没有考虑比例(延迟)作为更好的替代方案。在这封信中，在硫胺素依赖性酶的背景下比较了两种测量的统计特性。与相对增加相比，比例是一种更平衡、更敏感的度量，仿真结果表明，比例与相对增加相比具有相等或更高的统计能力。功率差可以高达0.12。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Comparison of Two Measures of Missing Cofactor in Cofactor-Dependent Enzymes: Proportion Versus Relative Increase

The degree of missing cofactor in a cofactor-dependent enzyme is widely used as a biomarker of cofactor defi- ciency. The degree of missing cofactor can be expressed either as the proportion of enzyme without cofactor, or as the rel- ative increase in enzyme with cofactor after addition of excess cofactor to the sample. Especially for enzymes with thia- mine pyrophosphate (TPP) as a cofactor, the relative increase (TPP-effect) has been used in a majority of studies, and its use seems to prevail without consideration of the proportion (latency) as a better alternative. In this letter, the statistical properties of the two measures are compared in the context of a thiamine-dependent enzyme. Proportion is a more bal- anced and sensitive measure than relative increase, and simulation shows that proportion is associated with equal or high- er statistical power than relative increase. The power difference can be as high as 0.12.

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来源期刊

Open Biomarkers Journal Medicine-Medicine (miscellaneous)

CiteScore

0.80

自引率

0.00%

发文量

期刊介绍： The Open Biomarkers Journal is an Open Access online journal, which publishes original full-length, short research articles and reviews on biomarkers in clinical, medical and pharmaceutical research. The coverage includes biomarkers of disease, new biomarkers, exposure to drugs, genetic effects, and applications of biomarkers. The Open Biomarkers Journal, a peer reviewed journal, aims to provide the most complete and reliable source of information on current developments in the field. The emphasis will be on publishing quality articles rapidly and freely available to researchers worldwide.