葡萄糖、硝酸盐和pH对小球藻培养的影响

IF 3.1 Q2 ENVIRONMENTAL SCIENCES
H. Nouri, J. M. Roushandeh, A. Hallajisani, A. Golzary, S. Daliry
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引用次数: 11

摘要

背景与目的:生物能源是近一个世纪以来引起人类关注的一种现象。小球藻的理想生物学特性使其成为生产生物柴油的最理想选择之一。但是,在其工业规模生产中必须考虑到经济问题。本研究旨在通过研究葡萄糖浓度作为碳源、硝酸盐浓度作为氮源和pH值作为三个最重要的因素对小球藻生物量生产和生长条件的影响。方法:采用响应面法进行试验设计,在葡萄糖浓度为2 ~ 20 g/L、硝酸盐浓度为0 ~ 1 g/L、硝酸盐浓度为6的条件下,同时考察各因素对玉米生长和生物量产量的影响。葡萄糖浓度为2.6 ~ 6 g/L,硝酸盐浓度为0.2 ~ 0.5 g/L, pH值为7 ~ 9时效果最佳。葡萄糖浓度为2.6 g/L,硝酸盐浓度为0.5 g/L, pH = 8时,生物量产量最高(1.31 g/L),特定生长率最高(0.167 1/d),生物量生产力最高(0.085 g/L/ d)。确定了最佳C/N比,pH值与生长速率变化呈显著相关。结论:小球藻在化学和物理参数上进行适当调整后,可作为一种有价值的生物质来源用于工业规模的生物柴油生产。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The effects of glucose, nitrate, and pH on cultivation of Chlorella sp. Microalgae
BACKGROUND AND OBJECTIVES: Bioenergy is a phenomenon that has attracted humans’ attention for about a century. The desirable biological properties of chlorella sp.microalgae have turned it to one of the most ideal options for the production of biodiesel. However, the economic issues must be taken into account in its industrial scale production. The present study aims to investigate chlorella sp. biomass production and growth conditions by studying the influence of glucose concentration as a carbon source, nitrate concentration as a nitrogen source and pH, as three of the most important factors. METHODS: For this purpose, design of experiment was done by response surface methodology and each factor was investigated simultaneously under glucose concentration in 2-20 g/L, nitrate concentration in 0-1 g/L and 6FINDINGS: The results indicated that carbon concentration has maximum effect on growth and biomass production. The best results were obtained in glucose concentration of 2.6-6 g/L, nitrate concentration of 0.2-0.5 g/L and pH values 7-9. Moreover, the maximum biomass production (1.31 g/L), the highest specific growth rate (0.167 1/day), and the highest biomass productivity (0.085 g/L/Day) were obtained in the following conditions: glucose concentration of 2.6 g/L, nitrate concentration of 0.5 g/L, and pH = 8. The optimal C/N ratio was determined and significant correlation was observed between pH and growth rate change. CONCLUSION: It was concluded that Chlorella sp ., if properly adjusted for both chemical and physical parameters could be a valuable source of biomass for biodiesel production in industrial scale.
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来源期刊
CiteScore
7.90
自引率
2.90%
发文量
11
审稿时长
8 weeks
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