特应性皮炎患儿母亲母乳中抗菌肽水平分析

Q3 Medicine

Voprosy Prakticheskoi Pediatrii Pub Date : 2022-01-01 DOI:10.20953/1817-7646-2022-6-106-111

A. Kudryavtseva, O. Svitich, V. Soboleva, E. Bystritskaya

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引用次数: 0

摘要

特应性皮炎(AD)是一种多因素疾病。阿尔茨海默病的发病与皮肤和肠道屏障功能衰竭及先天免疫平衡受损有关。先天免疫反应不足和上皮细胞抗菌肽(AMPs)的产生减少允许外来蛋白质和细菌毒素进入生物体，导致致敏和局部炎症。母乳可以作为抗菌肽的来源。用益生菌刺激上皮细胞可以增强不适当的抗菌保护。目标。探讨一种含嗜酸乳杆菌的益生菌对阿尔茨海默病婴儿母乳中β-防御素-2水平的影响。材料和方法。我们分析了含有乳酸菌菌株n.v. Ер 317/402«NARINE»的益生菌对阿尔茨海默病婴儿母亲母乳中β-防御素-2水平的影响。实验组(n = 26)包括患有AD婴儿的母亲。他们进一步分为两组:1组(n = 17)每天两次服用2粒益生菌胶囊(每粒180毫克)，持续20天，而2组(n = 9)每天两次服用2粒安慰剂，持续20天。对照组由健康婴儿的母亲组成(n = 11)。根据制造商的说明，使用防御素β 2 (DEFb2) ELISA试剂盒检测母乳中β-防御素-2的水平。使用未配对的学生T检验和韦尔奇T检验来评估差异。采用Microsoft Excel进行统计分析。结果。所分析的益生菌的建议剂量方案使患有AD的婴儿的母亲的母乳中β-防御素-2水平增加5.2倍(p < 0.05) (6.67 vs 1.29)，并减轻了婴儿的皮肤炎症。结论。乳杆菌sp.菌株n.v. Ер 317/402«NARINE»可能会影响母乳中β-防御素-2的水平。因此，益生菌可用于AD的预防和治疗。关键词:特应性皮炎，婴儿，防御素，母乳

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Analyzing the level of antimicrobial peptides in breast milk of mothers of infants with atopic dermatitis

Atopic dermatitis (AD) is a multifactorial disease. The onset of AD is associated with the skin and intestinal barrier failure and impaired balance of the innate immunity. Inadequate innate immune reactions and decreased production of antimicrobial peptides (AMPs) by epithelial cells allow the passage of foreign proteins and bacterial toxins into the organism resulting in sensitization and local inflammation. Breast milk can act as a source of AMPs. The improper antimicrobial protection can be enhanced by stimulating the epithelial cells with probiotics. Objective. To evaluate the effect of a probiotic containing Lactobacillus acidophilus on the level of β-defensin-2 in breast milk of mothers of infants with AD. Materials and methods. We analyzed the effect of a probiotic containing Lactobacillus sp. strain n.v. Ер 317/402 «NARINE» on the level of β-defensin-2 in breast milk of mothers of infants with AD. The experimental group (n = 26) included mothers of infants with AD. They were further divided into two groups: Group 1 (n = 17) received 2 capsules of probiotic (180 mg in one capsule) twice a day for 20 days, whereas Group 2 (n = 9) received 2 capsules of placebo twice a day for 20 days. The control group comprised mothers of healthy infants (n = 11). The level of β-defensin-2 in breast milk was measured using the ELISA Kit for Defensin Beta 2 (DEFb2) as per the manufacturer's instructions. The differences were evaluated using the unpaired Student's T test and Welch's t-test. Microsoft Excel was used for statistical analysis. Results. The proposed dosage regimen of the probiotic analyzed caused a 5.2-fold increase in the level of β-defensin-2 in breast milk of mothers of infants with AD (p < 0.05) (6.67 vs 1.29) and alleviation of skin inflammation in infants. Conclusion. Administration of Lactobacillus sp. strain n.v. Ер 317/402 «NARINE» may affect the level of β-defensin-2 in breast milk. Therefore, the probiotic can be used for the prevention and treatment of AD. Key words: atopic dermatitis, infants, defensins, breast milk

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来源期刊

Voprosy Prakticheskoi Pediatrii Medicine-Pediatrics, Perinatology and Child Health

CiteScore

1.20

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0.00%

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