宫颈癌新标志物MCM2和GINS2的鉴定

IF 0.5 4区生物学 Q4 ZOOLOGY

Pakistan Journal of Zoology Pub Date : 2023-01-01 DOI:10.17582/journal.pjz/20211116011144

L. Chu

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引用次数: 0

摘要

宫颈癌是女性最常见的恶性肿瘤，其预后较差。CC的关键基因和通路有待进一步发现。用GEO2R鉴定差异表达基因(DEG)，用DAVID分析GO和KEGG的富集程度。然后，使用STRINGS构造PPI网络。通过Cytoscape获得了DEGS的HUB基因和模块。最后，GEPIA还分析了关键基因的差异表达和存活情况。从GSE9750中提取234℃。子宫是集中分析的第四个突出的器官。DEGS的功能改变主要与细胞周期进程、细胞周期、解旋酶活性、DNA解旋酶活性、外泌体和p53信号通路有关。此外，还鉴定出5个HUB基因和1个关键模块。生存分析显示MCM2和GINS2与总生存显著相关。表达分析显示MCM2和GINS2在癌组织中高表达，在正常组织中低表达，与GEO分析结果一致。相关分析显示MCM2与GINS2呈显著正相关。本研究提示MCM2和GINS2可能是预测CC预后的新的生物标志物。

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Identification of Novel Biomarkers MCM2 and GINS2 for Cervical Cancer

Cervical cancer (CC) is the most common malignant tumor in women, and its prognosis is poor. The key genes and pathways of CC need to be further discovered. GEO2R was used to identify differentially expressed genes (DEG), GO and KEGG enrichment were analyzed by DAVID. Then, the PPI network is constructed with STRINGS. The HUB gene and module of DEGS were obtained by Cytoscape. Finally, GEPIA also analyzed the differential expression and survival of key genes. 234 DEG were extracted from GSE9750. The uterus is the fourth organ highlighted in the concentrated analysis. The functional changes of DEGS are mainly related to cell cycle progression, cell cycle, helicase activity, DNA helicase activity, exosome and p53 signal pathway. In addition, five HUB genes and one key module were identified. Survival analysis showed that MCM2 and GINS2 were significantly correlated with overall survival. Expression analysis showed that MCM2 and GINS2 were highly expressed in cancer tissues, but low in normal tissues, which was consistent with the results of GEO analysis. Correlation analysis showed that there was a significant positive correlation between MCM2 and GINS2. This study suggests that MCM2 and GINS2 may be new biomarkers to predict the prognosis of CC.

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来源期刊

Pakistan Journal of Zoology 生物-动物学

CiteScore

1.10

自引率

16.70%

发文量

306

审稿时长

4.5 months

期刊介绍： Pakistan Journal of Zoology (Pakistan J. Zool.) publishes original articles in English on all aspects of animal life. Generally these articles will be in, or related to one of the following subject areas: Physiology, Cell Biology, Molecular Biology, Genetics, Bioinformatics, Toxicology, Forensic Science, Developmental Biology, Entomology, Parasitology, Microbiology, Biotechnology, Pathology, Palaeontology. Taxonomy, Environmental Biology, Wildlife, Fisheries, Vertebrate and Invertebrate Morphology. Additionally, the journal considers research on health and clinical studies. Short communications are regularly considered, however, uninvited review articles, first records/reports of known species, case reports/studies and survey reports are not published in Pakistan Journal of Zoology.