正常和慢性反流性食管炎大鼠食管分层上皮的超微结构分析

Q4 Medicine

Archives of histology and cytology Pub Date : 2011-01-01 DOI:10.1679/AOHC.73.199

Hiroki Mori, M. Koike, T. Gotow, Koichiro Ichimura, D. Asaoka, M. Oguro, A. Nagahara, T. Ueno, Y. Uchiyama, Sumio Watanabe

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引用次数: 2

摘要

通讯地址:Hiroki Mori博士，Juntendo大学研究生院消化内科，2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan电话:+81-3-3813-3111，传真:+81-3-3813-8862 E-mail: hmori@juntendo.ac.jp TJ结构仅存在于对照大鼠食管上皮颗粒层(SG)， RE模型中TJ结构的数量似乎减少了。大多数tj只由一个“接吻点”组成。冷冻断裂电镜未发现典型的TJ链，提示大鼠食管黏膜TJ发育不全。免疫电镜证实了我们之前的免疫组化结果，cladin -3位于对照大鼠食管上皮细胞表面，RE组的免疫反应性降低。然而，claudin-3在质膜上弥漫性定位，而不是集中在TJ结构上。这些结果表明，claudin-3并不直接参与TJ结构的组成。

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Ultrastructural analyses of the rat esophageal stratified epithelium under normal conditions and in chronic reflux esophagitis

Address for correspondence: Dr. Hiroki Mori, Department of Gastroenterology, Juntendo University Graduate School of Medicine, 2-1-1 Hongo, Bunkyo-ku, Tokyo 113-8421, Japan Tel: +81-3-3813-3111, Fax: +81-3-3813-8862 E-mail: hmori@juntendo.ac.jp TJ structures only in the stratum granulosum (SG) of the esophageal epithelia in control rats, and the number of TJ structures seemed to be decreased in the RE model. Most of the TJs were composed of only one "kissing point." Freeze-fracture electron microscopy did not allow identification of typical TJ strands, suggesting that TJs in the rat esophageal mucosa were not well-developed. Immunoelectron microscopy confirmed our previous immunohistochemical results indicating that claudin-3 was located on the surface of esophageal epithelial cells in control rats and that the immunoreactivity decreased in the RE group. However, claudin-3 was diffusely localized on the plasma membrane and not concentrated on the TJ structures. These results indicate that claudin-3 is not directly involved in the composition of the TJ structure.

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来源期刊

Archives of histology and cytology 生物-细胞生物学

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期刊介绍： The Archives of Histology and Cytology provides prompt publication in English of original works on the histology and histochemistry of man and animals. The articles published are in principle restricted to studies on vertebrates, but investigations using invertebrates may be accepted when the intention and results present issues of common interest to vertebrate researchers. Pathological studies may also be accepted, if the observations and interpretations are deemed to contribute toward increasing knowledge of the normal features of the cells or tissues concerned. This journal will also publish reviews offering evaluations and critical interpretations of recent studies and theories.