Validation of the paper roll plus vermiculite (PR+V) germination test methodology for treated corn seeds

Abstract: A germination test is required by Brazilian standards for seed commercialization. The use of an adequate methodology that expresses the real physiological quality of seeds and minimizes possible phytotoxicity by chemicals is important for the production chain. In this context, the aim was to evaluate and validate the paper roll plus vermiculite (PR+V) germination test methodology for use in corn seeds. Two hybrids, one sensitive and the other tolerant to seed treatment, both with different vigor levels and under different chemical treatments, were used. Ten laboratories accredited by the Ministério da Agricultura e Pecuária (MAPA) were selected to perform and evaluate the PR+V germination tests. The data were subjected to statistical procedures to estimate the repeatability and reproducibility and check the accuracy and robustness of the test. The data were accurate, robust, and precise within the critical limits of 1 and 5%. The statistical results for repeatability, reproducibility, and bounds of the Mandel test were also accurate and precise at the critical limits of 1 and 5% for the normal seedlings evaluated by the PR+V methodology. Thus, the methodology can be used routinely to test corn seeds, especially those treated with phytosanitary products.