{"title":"滤纸抑制杂交大花蕙兰体外原球茎样体形成,降低合子萌发,但缓冲抗生素的负面影响","authors":"J. A. Teixeira da Silva","doi":"10.1515/biolet-2015-0005","DOIUrl":null,"url":null,"abstract":"Abstract Only few studies in the plant tissue culture literature have examined the impact of filter paper on in vitro plant organogenesis. In this study, using a model plant, hybrid Cymbidium Twilight Moon ‘Day Light’, the impact of a single or double layer of Advantec #2 or Whatman #1 filter paper on new protocorm-like body (neo-PLB) formation on Teixeira Cymbidium (TC) medium was examined for half-PLBs (transgenic and non-transgenic), PLB-derived transverse thin cell layers (tTCLs), and PLB synseeds. In addition, the response of half-PLBs or tTCLs to two antibiotics (kanamycin and cefotaxime, commonly used in plant genetic transformation studies) was investigated either directly on gelled medium or on filter paper-overlaid medium. Filter paper negatively affected most growth and developmental parameters of all the explants tested, both transgenic and non-transgenic. A double sheet of filter paper had a significantly (P ≤ 0.05) more negative impact than a single sheet, relative to the control values (i.e., no filter paper). Kanamycin inhibited neo-PLB formation on TC medium, the negative impact being greater on a single layer than on a double layer of filter paper, i.e., filter paper buffered the growth-inhibiting characteristics of kanamycin. Up to 100 mg/l, cefotaxime showed no apparent negative effects on neo-PLBs formation and growth, although hyperhydricity was observed when filter paper was not used.","PeriodicalId":37816,"journal":{"name":"Biological Letters","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1515/biolet-2015-0005","citationCount":"0","resultStr":"{\"title\":\"Filter paper inhibits in vitro protocorm-like body formation in hybrid Cymbidium and reduces synseed germination, but buffers the negative impact of antibiotics\",\"authors\":\"J. A. Teixeira da Silva\",\"doi\":\"10.1515/biolet-2015-0005\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Abstract Only few studies in the plant tissue culture literature have examined the impact of filter paper on in vitro plant organogenesis. In this study, using a model plant, hybrid Cymbidium Twilight Moon ‘Day Light’, the impact of a single or double layer of Advantec #2 or Whatman #1 filter paper on new protocorm-like body (neo-PLB) formation on Teixeira Cymbidium (TC) medium was examined for half-PLBs (transgenic and non-transgenic), PLB-derived transverse thin cell layers (tTCLs), and PLB synseeds. In addition, the response of half-PLBs or tTCLs to two antibiotics (kanamycin and cefotaxime, commonly used in plant genetic transformation studies) was investigated either directly on gelled medium or on filter paper-overlaid medium. Filter paper negatively affected most growth and developmental parameters of all the explants tested, both transgenic and non-transgenic. A double sheet of filter paper had a significantly (P ≤ 0.05) more negative impact than a single sheet, relative to the control values (i.e., no filter paper). Kanamycin inhibited neo-PLB formation on TC medium, the negative impact being greater on a single layer than on a double layer of filter paper, i.e., filter paper buffered the growth-inhibiting characteristics of kanamycin. Up to 100 mg/l, cefotaxime showed no apparent negative effects on neo-PLBs formation and growth, although hyperhydricity was observed when filter paper was not used.\",\"PeriodicalId\":37816,\"journal\":{\"name\":\"Biological Letters\",\"volume\":null,\"pages\":null},\"PeriodicalIF\":0.0000,\"publicationDate\":\"2014-01-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"https://sci-hub-pdf.com/10.1515/biolet-2015-0005\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Biological Letters\",\"FirstCategoryId\":\"1085\",\"ListUrlMain\":\"https://doi.org/10.1515/biolet-2015-0005\",\"RegionNum\":0,\"RegionCategory\":null,\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"Agricultural and Biological Sciences\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biological Letters","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1515/biolet-2015-0005","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
Filter paper inhibits in vitro protocorm-like body formation in hybrid Cymbidium and reduces synseed germination, but buffers the negative impact of antibiotics
Abstract Only few studies in the plant tissue culture literature have examined the impact of filter paper on in vitro plant organogenesis. In this study, using a model plant, hybrid Cymbidium Twilight Moon ‘Day Light’, the impact of a single or double layer of Advantec #2 or Whatman #1 filter paper on new protocorm-like body (neo-PLB) formation on Teixeira Cymbidium (TC) medium was examined for half-PLBs (transgenic and non-transgenic), PLB-derived transverse thin cell layers (tTCLs), and PLB synseeds. In addition, the response of half-PLBs or tTCLs to two antibiotics (kanamycin and cefotaxime, commonly used in plant genetic transformation studies) was investigated either directly on gelled medium or on filter paper-overlaid medium. Filter paper negatively affected most growth and developmental parameters of all the explants tested, both transgenic and non-transgenic. A double sheet of filter paper had a significantly (P ≤ 0.05) more negative impact than a single sheet, relative to the control values (i.e., no filter paper). Kanamycin inhibited neo-PLB formation on TC medium, the negative impact being greater on a single layer than on a double layer of filter paper, i.e., filter paper buffered the growth-inhibiting characteristics of kanamycin. Up to 100 mg/l, cefotaxime showed no apparent negative effects on neo-PLBs formation and growth, although hyperhydricity was observed when filter paper was not used.
Biological LettersAgricultural and Biological Sciences-Agricultural and Biological Sciences (all)
自引率
0.00%
发文量
0
审稿时长
52 weeks
期刊介绍:
It covers a wide spectrum of biological disciplines, including animal science and zoology, general biochemistry, botany, and ecology. We also encourage submission of manuscripts related to conservation biology and wildlife management.