马来西亚登嘉楼热当岛受黑带病影响珊瑚组织中细菌群落的分离和鉴定

IF 0.6 4区 环境科学与生态学 Q4 ECOLOGY
S. Jaafar, M. Suhaimi, N. A. Maznan, C. Safuan, C. Tan, M. Yucharoen, J. Saidin, Z. Bachok
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引用次数: 0

摘要

. 由于自然和人为干扰,导致水质恶化,威胁到珊瑚礁的健康,东南亚的珊瑚礁正在逐步退化。珊瑚病是世界范围内对珊瑚礁的威胁之一,但对东南亚珊瑚病的研究相对较少,特别是对与珊瑚病有关的细菌群落的研究。破坏性珊瑚黑带病(BBD)广泛分布于马来西亚南海南部海域。本研究旨在比较在登嘉诺岛热当岛鲨鱼角采集的两种不同珊瑚物种的健康珊瑚和感染bbd的珊瑚组织中的微生物群落。采用培养依赖法和细菌16S rDNA测序相结合的方法对从珊瑚环中分离的细菌进行了检测。在继代培养菌落样本之前,从两种珊瑚组织样本中共鉴定出18种细菌分离物。检测到的序列来自广泛的分类范围,包括放线菌门、真菌门和变形菌门的代表。属于变形菌门的细菌进一步分为α和γ变形菌门。大多数已鉴定的细菌可在健康和受bbd影响的珊瑚组织样本中发现;然而,很少有细菌只能在各自的珊瑚物种的受bbd影响的组织中发现,如M. turtlensis的Lelliottia sp., Salnicola salarius和Microbacterium petrolearium,而A. spicifera的stutzeri假单胞菌和红杆菌sp.。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
ISOLATION AND IDENTIFICATION OF BACTERIAL COMMUNITIES FROM CORAL TISSUE AFFECTED BY BLACK BAND DISEASE AT PULAU REDANG, TERENGGANU, MALAYSIA
. Coral reefs in Southeast Asia are becoming progressively degraded due to the natural and anthropogenic disturbances which lead to poor water quality threatening the reefs’ health. Coral disease is one of the threats to the coral reefs worldwide but research on coral disease in Southeast Asia is relatively scarce especially on bacterial community associated with the disease. The destructive coral black band disease (BBD) can be found widely in the southern region of the South China Sea, Malaysia. This study aims to compare the microbial communities of healthy and BBD-infected coral tissue from two different coral species Montipora turtlensis and Acropora spicifera collected at the Shark Point of Pulau Redang, Terengganu. The bacterial isolated from corals nubbins were examined by combining culture-dependent method and bacterial sequencing of 16S rDNA. A total of 18 bacteria isolates were identified from both species of coral tissue samples prior to sub-culturing colonies samples. The sequences detected were derived from a wide taxonomic range, including representatives of Actinobacteria, Furmicutes and Proteobacteria phyla. Bacteria belonging to the phyla Proteobacteria were further divided into alpha and gammaproteobacteria. Most of the bacteria identified can be found on healthy and BBD-affected coral tissue samples; however, there are few bacteria which can be only found on BBD-affected tissue of respective coral species such as Lelliottia sp., Salnicola salarius and Microbacterium petrolearium for M. turtlensis while Pseudomonas stutzeri and Erythrobacter sp. for A. spicifera.
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来源期刊
Applied Ecology and Environmental Research
Applied Ecology and Environmental Research ECOLOGY-ENVIRONMENTAL SCIENCES
CiteScore
1.40
自引率
14.30%
发文量
104
审稿时长
14 months
期刊介绍: The Journal publishes original research papers and review articles. Researchers from all countries are invited to publish pure or applied ecological, environmental, biogeographical, zoological, botanical, paleontological, biometrical-biomathematical and quantitative ecological or multidisciplinary agricultural research of international interest on its pages. The focus is on topics such as: -Community, ecosystem and global ecology- Biometrics, theoretical- and quantitative ecology- Multidisciplinary agricultural and environmental research- Sustainable and organic agriculture, natural resource management- Ecological methodology, monitoring and modeling- Biodiversity and ecosystem research, microbiology, botany and zoology- Biostatistics and modeling in epidemiology, public health and veterinary- Earth history, paleontology, extinctions, biogeography, biogeochemistry- Conservation biology, environmental protection- Ecological economics, natural capital and ecosystem services- Climatology, meteorology, climate change, climate-ecology. The Journal publishes theoretical papers as well as application-oriented contributions and practical case studies. There is no bias with regard to taxon or geographical area. Purely descriptive papers (like only taxonomic lists) will not be accepted for publication.
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